Abstract
Over the past few decades, optical tweezers have become a powerful tool that is widely used in cell-based biomedical applications. Their popularity is attributed to their unique advantages in the manipulation of biological cells with high accuracy, degree of freedom, and flexibility in a noninvasive manner. With the trends toward the automation of biological processes with high throughput, precision, and reliability, many autonomous frameworks have been developed for the realization of diverse cell manipulations. This study reviews the latest advancements in automated cell transportation and reorientation control. Moreover, by integrating optical tweezers with other tools, the mechanisms of cell-based physiological activity and subcellular operation are investigated and reviewed. Discussions on the current challenges and potential research trends on optical manipulation of biological cells are finally presented.
Similar content being viewed by others
References
Ashkin A (1970) Acceleration and trapping of particles by radiation pressure. Phys Rev Lett 24:156–159
Ashkin A, Dziedzic J, Bjorkholm J, Chu S (1986) Observation of a single-beam gradient force optical trap for dielectric particles. Opt Lett 11:288–290
Wu Y, Sun D, Huang W, Xi N (2013) Dynamics analysis and motion planning for automated cell transportation with optical tweezers. IEEE/ASME Trans Mech 18:706–713
Grover SC, Skirtach AG, Gauthier RC, Grover CP (Jan 2001) Automated single-cell sorting system based on optical trapping. J Biomed Opt 6:14–22
Hendricks AG, Goldman YE (2017) Measuring molecular forces using calibrated optical tweezers in living cells. Meth Mole Bio 1486(2017):537–552
Grammatikopoulou M and Yang G (2019) Three-dimensional pose estimation of optically transparent microrobots, IEEE Robotics and Automation Letters, pp. 1-1.
Grammatikopoulou M and Yang G (2017) Gaze contingent control for optical micromanipulation, in 2017 IEEE International Conference on Robotics and Automation (ICRA), pp. 5989-5995.
Ashkin A, Dziedzic J (1987) Optical trapping and manipulation of viruses and bacteria. Science 235:1517–1520
Dasgupta R, Verma RS, Ahlawat S, Uppal A, Gupta PK (2011) Studies on erythrocytes in malaria infected blood sample with Raman optical tweezers. J Biomed Opt 16
Gou X, Yang H, Fahmy TM, Wang Y, Sun D (2014) Direct measurement of cell protrusion force utilizing a robot-aided cell manipulation system with optical tweezers for cell migration control. Int J Robot Res 33:1782–1792
Galla L, Meyer AJ, Spiering A, Sischka A, Mayer M, Hall AR, Reimann P, Anselmetti D (Jul 2014) Hydrodynamic slip on DNA observed by optical tweezers-controlled translocation experiments with solid-state and lipid-coated nanopores. Nano Lett 14:4176–4182
Heller I, Hoekstra TP, King GA, Peterman EJG, Wuite GJL (2014) Optical tweezers analysis of DNA-protein complexes. Chem Rev 114:3087–3119
Padgett M, Di Leonardo R (2011) Holographic optical tweezers and their relevance to lab on chip devices. Lab Chip 11:1196–1205
Chen H, Sun D (2012) Moving groups of microparticles into array with a robot–tweezers manipulation system. IEEE Trans Robot 28:1069–1080
Cheah CC, Li X, Yan X, Sun D (2014) Observer-based optical manipulation of biological cells with robotic tweezers. IEEE Trans Robot 30:68–80
Chowdhury S, Thakur A, Svec P, Wang C, Losert W, Gupta SK (2014) Automated manipulation of biological cells using gripper formations controlled by optical tweezers. IEEE Trans Autom Sci Eng 11:338–347
Wang X, Chen S, Kong M, Wang Z, Costa KD, Li RA, Sun D (Nov 7 2011) Enhanced cell sorting and manipulation with combined optical tweezer and microfluidic chip technologies. Lab Chip 11:3656–3662
Chen S, Cheng J, Kong C-W, Wang X, Han Cheng S, Li RA et al (2013) Laser-induced fusion of human embryonic stem cells with optical tweezers. Appl Phys Lett 103:033701
Tan Y, Sun D, Cheng SH, and Li R.A (2011) Robotic cell manipulation with optical tweezers for biomechanical characterization, presented at the Robotics and Automation (ICRA), 2011 IEEE International Conference on.
Binnig G, Quate CF, Gerber C (1986) Atomic force microscope. Phys Rev Lett 56:930
Hörber J, Miles M (2003) Scanning probe evolution in biology. Science 302:1002–1005
Greenleaf WJ, Woodside MT, Block SM (2007) High-resolution, single-molecule measurements of biomolecular motion. Annu Rev Biophys Biomol Struct 36:171
Charras GT, Horton MA (2002) Single cell mechanotransduction and its modulation analyzed by atomic force microscope indentation. Biophys J 82:2970–2981
Zhang C, Khoshmanesh K, Mitchell A, Kalantar-Zadeh K (2010) Dielectrophoresis for manipulation of micro/nano particles in microfluidic systems. Anal Bioanal Chem 396:401–420
Pethig R (2010) Review article—dielectrophoresis: status of the theory, technology, and applications. Biomicrofluidics 4:022811
Morgan H, Hughes MP, Green NG (1999) Separation of submicron bioparticles by dielectrophoresis. Biophys J 77:516–525
Jiang C, Mills JK (2015) Planar cell orientation control system using a rotating electric field. IEEE/ASME Trans Mech 20:2350–2358
Chu H, Huan Z, Mills J, Yang J, Sun D (2015) Three-dimensional cell manipulation and patterning using dielectrophoresis via a multi-layer scaffold structure. Lab Chip 15:920–930
Allsopp D, Milner K, Brown A, Betts W (1999) Impedance technique for measuring dielectrophoretic collection of microbiological particles. J Phys D Appl Phys 32:1066
Heo S, Kim YY (Nov 2007) Optimal design and fabrication of MEMS rotary thermal actuators. J Micromech Microeng 17:2241–2247
Ding X, Lin S-CS, Kiraly B, Yue H, Li S, Chiang I-K et al (2012) On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves. Proc Natl Acad Sci 109:11105–11109
Neuman KC, Block SM (2004) Optical trapping. Rev Sci Instrum 75:2787–2809
Ramser K, Hanstorp D (2010) Optical manipulation for single-cell studies. J Biophotonics 3:187–206
Svoboda K, Block SM (1994) Biological applications of optical forces. Annu Rev Biophys Biomol Struct 23:247–285
Misawa H, Sasaki K, Koshioka M, Kitamura N, Masuhara H (1992) Multibeam laser manipulation and fixation of microparticles. Appl Phys Lett 60:310–312
Sasaki K, Koshioka M, Misawa H, Kitamura N, Masuhara H (1991) Pattern formation and flow control of fine particles by laser-scanning micromanipulation. Opt Lett 16:1463–1465
Visscher K, Gross SP, Block SM (1996) Construction of multiple-beam optical traps with nanometer-resolution position sensing. Selected Topics in Quantum Electronics, IEEE Journal 2:1066–1076
Curtis JE, Schmitz CH, Spatz JP (2005) Symmetry dependence of holograms for optical trapping. Opt Lett 30:2086–2088
Polin M, Ladavac K, Lee S-H, Roichman Y, Grier D (2005) Optimized holographic optical traps. Opt Express 13:5831–5845
Hu S, Sun D (2011) Automatic transportation of biological cells with a robot-tweezer manipulation system. Int J Robot Res 30:1681–1694
Ju T, Liu S, Yang J, Sun D (2014) Rapidly exploring random tree algorithm-based path planning for robot-aided optical manipulation of biological cells. IEEE Trans Autom Sci Eng 11:649–657
Yan X, Sun D (2015) Multilevel-based topology design and cell patterning with robotically controlled optical tweezers. IEEE Trans Control Syst Technol 23:176–185
Chen H, Wang C, Lou Y (2013) Flocking multiple microparticles with automatically controlled optical tweezers: solutions and experiments. IEEE Trans Biomed Eng 60:1518–1527
Xie M, Wang Y, Feng G, Sun D (2015) Automated pairing manipulation of biological cells with a robot-tweezers manipulation system. IEEE/ASME Trans Mech 20:2242–2251
Li X, Yang H, Wang J, Sun D (2015) Design of a robust unified controller for cell manipulation with a robot-aided optical tweezers system. Automatica 55:279–286
Li X, Yang H, Huang H, Sun D (2018) A switching controller for high speed cell transportation by using a robot-aided optical tweezers system. Automatica 89:308–315
Hu S, Chen S, Chen S, Xu G, Sun D (2017) Automated transportation of multiple cell types using a robot-aided cell manipulation system with holographic optical tweezers. IEEE/ASME Trans Mech 22:804–814
Xie M, Li X, Wang Y, Liu Y, Sun D (2018) Saturated PID control for the optical manipulation of biological cells. IEEE Trans Control Syst Technol 26:1909–1916
Cheah CC, Li X, Yan X, Sun D (2015) Simple PD control scheme for robotic manipulation of biological cell. IEEE Trans Autom Control 60:1427–1432
Li X, Cheah CC, Hu S, Sun D (2013) Dynamic trapping and manipulation of biological cells with optical tweezers. Automatica 49:1614–1625
Li X, Cheah CC (2015) Robotic cell manipulation using optical tweezers with unknown trapping stiffness and limited FOV. IEEE/ASME Trans Mech 20:1624–1632
Li X, Cheah CC (Dec 2017) Stochastic optical trapping and manipulation of a micro object with neural-network adaptation. IEEE/ASME Trans Mech 22:2633–2642
Li X, Cheah CC (2017) A simple trapping and manipulation method of biological cell using robot-assisted optical tweezers: singular perturbation approach. IEEE Trans Ind Electron 64:1656–1663
Yan X, Cheah CC, Ta QM, Pham QC (Jun 2016) Stochastic dynamic trapping in robotic manipulation of micro-objects using optical tweezers. IEEE Trans Robot 32:499–512
Xie M, Shakoor A, Li C, Sun D (2019) Robust orientation control of multi-DOF Cell based on uncertainty and disturbance estimation. Int J Robust Nonlin Contr 9(14):4859–4871
Li X, Liu C, Chen S, Wang Y, Cheng SH, Sun D (2017) In vivo manipulation of single biological cells with an optical tweezers-based manipulator and a disturbance compensation controller. IEEE Trans Robot 33:1200–1212
Li X, Chen S, Liu C, Cheng SH, Wang Y, Sun D (2018) Development of a collision-avoidance vector based control algorithm for automated in-vivo transportation of biological cells. Automatica 90:147–156
Li X, Xu S, Cheng SH, and D. Sun (2019) Simultaneous localization and mapping-based in vivo navigation control of microparticles, IEEE Transactions on Industrial Informatics, pp. 1-1.
Aabo T, Perch-Nielsen IR, Dam JS, Palima DZ, Siegumfeldt H, GlÞckstad J et al (2010) Effect of long-and short-term exposure to laser light at 1070 nm on growth of saccharomyces cerevisiae. J Biomed Opt 15:041505–041505-7
Banerjee AG, Chowdhury S, Gupta SK, Losert W (2011) Survey on indirect optical manipulation of cells, nucleic acids, and motor proteins. J Biomed Opt 16:051302
Sun CK, Huang YC, Cheng PC, Liu HC, Lin BL (Oct 2001) Cell manipulation by use of diamond microparticles as handles of optical tweezers. J Opt Soc Am B-Optical Phys 18:1483–1489
Ta QM, Cheah CC (2019) Stochastic control for orientation and transportation of microscopic objects using multiple optically driven robotic fingertips. IEEE Trans Robot 35:861–872
Thakur A, Chowdhury S, Švec P, Wang C, Losert W, Gupta SK (2014) Indirect pushing based automated micromanipulation of biological cells using optical tweezers. Int J Robot Res 33:1098–1111
Chowdhury S, Thakur A, Wang C, Švec P, Losert W, and Gupta SK, Automated indirect manipulation of irregular shaped cells with optical tweezers for studying collective cell migration, in Robotics and Automation (ICRA), 2013 IEEE International Conference on, 2013, pp. 2789-2794.
Banerjee A, Chowdhury S, Gupta SK (2014) Optical tweezers: autonomous robots for the manipulation of biological cells. IEEE Robot Autom Mag 21:81–88
Xie Y, Sun D, Liu C, Tse HY, Cheng SH (2010) A force control approach to a robot-assisted cell microinjection system. Int J Robot Res 29:1222–1232
Ebner T, Moser M, Shebl O, Mayer R, Tews G (2011) Assisting in vitro fertilization by manipulating cumulus-oocyte-complexes either mechanically or enzymatically does not prevent IVF failure. J Turk Ger Gynecol Assoc 12:135
Yoshida N, Perry AC (2007) Piezo-actuated mouse intracytoplasmic sperm injection (ICSI). Nat Protoc 2:296–304
Ando J, Bautista G, Smith N, Fujita K, Daria VR (Oct 2008) Optical trapping and surgery of living yeast cells using a single laser. Rev Sci Instrum 79:103705
Mohanty SK, Uppal A, Gupta PK (2004) Self-rotation of red blood cells in optical tweezers: prospects for high throughput malaria diagnosis. Biotechnol Lett 26:971–974
Mohanty SK, Dasgupta R, Gupta PK (2005) Three-dimensional orientation of microscopic objects using combined elliptical and point optical tweezers. Appl Phys B Lasers Opt 81:1063–1066
Arias A, Etcheverry S, Solano P, Staforelli J, Gallardo MJ, Rubinsztein-Dunlop H, Saavedra C (2013) Simultaneous rotation, orientation and displacement control of birefringent microparticles in holographic optical tweezers. Opt Express 21:102–111
Bingelyte V, Leach J, Courtial J, Padgett M (2003) Optically controlled three-dimensional rotation of microscopic objects. Appl Phys Lett 82:829–831
Cao B, Kelbauskas L, Chan S, Shetty RM, Smith D, Meldrum DR (2017) Rotation of single live mammalian cells using dynamic holographic optical tweezers. Opt Lasers Eng 92:70–75
Tanaka Y, Kawada H, Hirano K, Ishikawa M, Kitajima H (2008) Automated manipulation of non-spherical micro-objects using optical tweezers combined with image processing techniques. Opt Express 16:15115–15122
Sheu F-W, Lan T-K, Lin Y-C, Chen S, Ay C (2010) Stable trapping and manually controlled rotation of an asymmetric or birefringent microparticle using dual-mode split-beam optical tweezers. Opt Express 18:14724–14729
Ta QM and Cheah CC, Simultaneous orientation and positioning control of a microscopic object using robotic tweezers, in Robotics and Automation (ICRA), 2017 IEEE International Conference on, 2017, pp. 5864-5869.
Xie M, Mills JK, Wang Y, Mahmoodi M, Sun D (2016) Automated translational and rotational control of biological cells with a robot-aided optical tweezers manipulation system. IEEE Trans Autom Sci Eng 13:543–551
Xie M, Mills JK, Li X, Wang Y, and Sun D, Modelling and control of optical manipulation for cell rotation, in Robotics and Automation (ICRA), 2015 IEEE International Conference on, 2015, pp. 956-961.
Xie M, Shakoor A, Shen Y, Mills JK, Sun D (2019) Out-of-plane rotation control of biological cells with a robot-tweezers manipulation system for orientation-based cell surgery. IEEE Trans Biomed Eng 66:199–207
Zhuang S, Lin W, Zhong J, Zhang G, Li L, Qiu J, Gao H (Jan 2018) Visual servoed three-dimensional rotation control in zebrafish larva heart microinjection system. IEEE Trans Biomed Eng 65:64–73
Leung C, Lu Z, Zhang XP, Sun Y (2012) Three-dimensional rotation of mouse embryos. IEEE Trans Biomed Eng 59:1049–1056
Kotnala A, Zheng Y, Fu J, Cheng W (2017) Microfluidic-based high-throughput optical trapping of nanoparticles. Lab Chip 17:2125–2134
Pilát Z, Jonáš A, Ježek J, Zemánek P (2017) Effects of infrared optical trapping on saccharomyces cerevisiae in a microfluidic system. Sensors 17:2640
Umehara S, Hattori A, Wakamoto Y, Yasuda K (2004) Simultaneous measurement of growth and movement of cells exploiting on-chip single-cell cultivation assay. Jpn J Appl Phys 43:1214
Eriksson E, Enger J, Nordlander B, Erjavec N, Ramser K, Goksör M, Hohmann S, Nyström T, Hanstorp D (2007) A microfluidic system in combination with optical tweezers for analyzing rapid and reversible cytological alterations in single cells upon environmental changes. Lab Chip 7:71–76
Wang MM, Tu E, Raymond DE, Yang JM, Zhang H, Hagen N et al (2005) Microfluidic sorting of mammalian cells by optical force switching. Nat Biotechnol 23:83
Shields CW IV, Reyes CD, López GP (2015) Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation. Lab Chip 15:1230–1249
Tan Y, Sun D, Wang J, Huang W (2010) Mechanical characterization of human red blood cells under different osmotic conditions by robotic manipulation with optical tweezers. IEEE Trans Biomed Eng 57:1816–1825
Seeger S, Monajembashi S, Hutter KJ, Futterman G, Wolfrum J, Greulich K (1991) Application of laser optical tweezers in immunology and molecular genetics. J Int Soc Anal Cyto 12:497–504
Shakoor A, Xie M, Luo T, Hou J, Shen Y, Mills JK, Sun D (2019) Achieving automated organelle biopsy on small single cells using a cell surgery robotic system. IEEE Trans Biomed Eng 66:2210–2222
Shakoor A, Luo T, Chen S, Xie M, Mills JK, and Sun D (2017) A high-precision robot-aided single-cell biopsy system, in 2017 IEEE International Conference on Robotics and Automation (ICRA), pp. 5397-5402.
Schneckenburger H, Hendinger A, Sailer R, Gschwend MH, Strauss WS, Bauer M, Schütze K (2000) Cell viability in optical tweezers: high power red laser diode versus Nd: YAG laser. J Biomed Opt 5:40–44
Min TL, Mears PJ, Chubiz LM, Rao CV, Golding I, Chemla YR (2009) High-resolution, long-term characterization of bacterial motility using optical tweezers. Nat Methods 6:831
Jiao J, Rebane AA, Ma L, and Zhang Y (2017) Single-molecule protein folding experiments using high-precision optical tweezers, in Optical Tweezers, ed: Springer, pp. 357-390.
Funding
State Administration of Foreign Experts Affairs (G20190010180). Basic Research Program of Jiangsu Province (BK20180427). Fundamental Research Funds for the Central Universities (NS2019021). National Aerospace Science Foundation of China (2018ZD52050).
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Xie, M. Autonomous robot-aided optical tweezer system for biological cell manipulation. Int J Adv Manuf Technol 105, 4953–4966 (2019). https://doi.org/10.1007/s00170-019-04683-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00170-019-04683-1