Knee Surgery, Sports Traumatology, Arthroscopy

, Volume 26, Issue 8, pp 2430–2437 | Cite as

ACL graft compression: a method to allow reduced tunnel sizes in ACL reconstruction

  • Breck R. Lord
  • Henry B. Colaco
  • Chinmay M. Gupte
  • Adrian J. Wilson
  • Andrew A. Amis



A common problem during ACL reconstruction is asymmetry of proximal–distal graft diameter leading to tunnel upsizing and graft–tunnel mismatch. Compression downsizing provides a graft of uniform size, allowing easy passage into a smaller tunnel. The purpose of this study was to quantify the graft compression technique and its effects on graft biomechanics and stability. It was hypothesised that compression downsizing would significantly reduce cross-sectional area (CSA); that no significant changes in graft biomechanics would occur; graft fixation stability would be improved.


Sixty-eight non-irradiated peroneus longus (PL) tendons were investigated. Twenty were halved and paired into ten four-strand grafts, 20 strands were compressed by 0.5–1 mm diameter and changes in CSA recorded using an alginate mould technique. The following properties were compared with 20 control strands: cyclic strain when loaded 70–220 N for 1000 cycles; stiffness; ultimate tensile load and stress; Young’s modulus. 24 PL tendons were quadrupled into grafts, 12 were compressed and all 24 were submerged in Ringer’s solution at 37 °C and the CSA recorded over 12 h. Twelve compressed and 12 control quadrupled grafts were mounted in porcine femurs, placed in Ringer’s solution for 12 h at 37 °C and graft displacement at the bone tunnel aperture recorded under cyclic loading.


Mean decreases in CSA of 31% under a stress of 471 kPa and 21% under a stress of 447 kPa were observed for doubled and quadrupled grafts, respectively. Compressed grafts re-expanded by 19% over 12 h compared to 2% for controls. No significant differences were observed between compressed and control grafts in the biomechanical properties and graft stability; mean cyclic displacements were 0.3 mm for both groups.


No detrimental biomechanical effects of graft compression on allograft PL tendons were observed. Following compression, the grafts significantly increased in size during in vitro joint simulation. No significant difference was observed in graft stability between groups. Graft compression did not cause adverse mechanical effects in vitro. Smaller tunnels for compressed grafts reduce bone loss and ease anatomical placement.


Anterior cruciate ligament ACL reconstruction Compression downsizing Tendon graft biomechanics 



Anterior cruciate ligament


Anterior cruciate ligament reconstruction


Analysis of variance


Cross-sectional area


Intraclass correlation coefficients




Peroneus longus


Standard deviation


Statistical Package for the Social Sciences


United States of America


Ultimate failure load


Ultimate tensile stress



BRL was supported by the Orthopaedic Research Fund of the North Hampshire Hospital. The Instron materials testing machine was donated by the Arthritis Research UK charity. The tendon specimens were donated by RTI Surgical Co., Florida, USA.

Compliance with ethical standards

Conflict of interest

None declared.

Ethical approval

This study was approved by the Imperial College Healthcare Tissue Bank, HTA licence 12275, REC Wales approval 12/WA/0196, project R13058.

Informed consent

Not required for this study, which was covered by a Research Ethics Committee permit.


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Copyright information

© European Society of Sports Traumatology, Knee Surgery, Arthroscopy (ESSKA) 2018

Authors and Affiliations

  1. 1.The Biomechanics Group, Department of Mechanical EngineeringImperial College LondonLondonUK
  2. 2.Basingstoke and North Hampshire HospitalBasingstokeUK
  3. 3.St Georges HospitalLondonUK
  4. 4.Musculoskeletal Surgery Group, Department of Surgery and CancerImperial College London School of Medicine, Charing Cross HospitalLondonUK

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