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Multiple organ failure (MOF) after severe trauma — A sheep model

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Abstract

Objective: To perform a reproducible long-term (10 days) large animal model of multiple systems organ failure without necessity of a continuous stimulus.

Design: Adult female merino sheep submitted to a 5-day stimulation period followed by a 5-day observation period. Day 1: Hemorrhagic shock was combined with a traumatic surgical insult (reamed intra-medullary femoral nailing), followed by serial administrations every 12 h for 5 days of a combination of endotoxin and zymosan activated plasma. Organ function was followed for 5 further days.

Results: Cardiac index increased significantly during the study (day 1: 491 ± 8 mmHg; day 10: 427 ± 20, p<0.05). Liver function was impaired and bilirubin levels increased significantly (day 1: 2.9 ± 0.3 µmol/1; day 10: 7.2 ± 0.9; p<0.05). Creatinine clearance decreased initially (day 1: 54 ± 7 ml/min), increased to a peak on day 2 (104 ± 27), and then deteriorated again (day 10: 53 ± 18).

Conclusion: This new large animal model of trauma-induced MOF is reproducible and may be suitable for the study of new therapeutic approaches to therapy.

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Authors and Affiliations

  1. Department of Trauma Surgery, Hannover Medical School, Carl Neubergstr. 1, D-30625, Hannover, Germany

    H. C. Pape, M. Grotz, D. Remmers & H. Tscherne

  2. Department of Clinical Biochemistry, Hannover Medical School, Carl Neubergstr. 1, D-30625, Hannover, Germany

    A. Dwenger

  3. Department of Veterinary Pathology, Hannover Medical School, Carl Neubergstr. 1, D-30625, Hannover, Germany

    R. Vaske

  4. Department of Surgery, University of California, Davis, Sacramento, USA

    D. Wisner

Authors
  1. H. C. Pape
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  2. M. Grotz
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  3. D. Remmers
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  4. A. Dwenger
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  5. R. Vaske
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  6. D. Wisner
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  7. H. Tscherne
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Supported by “Deutsche Forschungsge-meinschaft”, Proj. No. Re 1015/1-1 and /1-2

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Pape, H.C., Grotz, M., Remmers, D. et al. Multiple organ failure (MOF) after severe trauma — A sheep model. Intensive Care Med 24, 590–598 (1998). https://doi.org/10.1007/s001340050620

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