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Molecular diagnostics in severe pneumonia: a new dawn or false promise?

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References

  1. Vincent J-L, Sakr Y, Singer M et al (2020) Prevalence and outcomes of infection among patients in intensive care units in 2017. JAMA 323:1478–1487. https://doi.org/10.1001/jama.2020.2717

    Article  PubMed  PubMed Central  Google Scholar 

  2. Meduri GU, Mauldin GL, Wunderink RG et al (1994) Causes of fever and pulmonary densities in patients with clinical manifestations of ventilator-associated pneumonia. Chest 106:221–235. https://doi.org/10.1378/chest.106.1.221

    Article  CAS  PubMed  Google Scholar 

  3. Metlay JP, Waterer GW, Long AC et al (2019) Diagnosis and treatment of adults with community-acquired pneumonia. an official clinical practice guideline of the American Thoracic Society and Infectious Diseases Society of America. Am J Respir Crit Care Med 200:e45–e67. https://doi.org/10.1164/rccm.201908-1581ST

    Article  PubMed  PubMed Central  Google Scholar 

  4. Woodhead M, Blasi F, Ewig S et al (2011) Guidelines for the management of adult lower respiratory tract infections–full version. Clin Microbiol Infect 17(Suppl 6):E1-59. https://doi.org/10.1111/j.1469-0691.2011.03672.x

    Article  PubMed  PubMed Central  Google Scholar 

  5. Roquilly A, Torres A, Villadangos JA et al (2019) Pathophysiological role of respiratory dysbiosis in hospital-acquired pneumonia. Lancet Respir Med. https://doi.org/10.1016/S2213-2600(19)30140-7

    Article  PubMed  Google Scholar 

  6. Hanson KE, Azar MM, Banerjee R et al (2020) Molecular testing for acute respiratory tract infections: clinical and diagnostic recommendations from the IDSA’s Diagnostics Committee. Clin Infect Dis 71:2744–2751. https://doi.org/10.1093/cid/ciaa508

    Article  CAS  PubMed  Google Scholar 

  7. Morris AC, Kefala K, Simpson AJ et al (2009) Evaluation of the effect of diagnostic methodology on the reported incidence of ventilator-associated pneumonia. Thorax 64:516–522. https://doi.org/10.1136/thx.2008.110239

    Article  PubMed  Google Scholar 

  8. Jones NK, Conway Morris A, Curran MD et al (2020) Evaluating the use of a 22-pathogen TaqMan array card for rapid diagnosis of respiratory pathogens in intensive care. J Med Microbiol 69:971–978. https://doi.org/10.1099/jmm.0.001218

    Article  PubMed  Google Scholar 

  9. Murphy CN, Fowler R, Balada-Llasat JM et al (2020) Multicenter evaluation of the biofire filmarray pneumonia/pneumonia plus panel for detection and quantification of agents of lower respiratory tract infection. J Clin Microbiol. https://doi.org/10.1128/JCM.00128-20

    Article  PubMed  PubMed Central  Google Scholar 

  10. Anjum MF, Zankari E, Hasman H (2017) molecular methods for detection of antimicrobial resistance. Microbiol Spectr. https://doi.org/10.1128/microbiolspec.ARBA-0011-2017

    Article  PubMed  Google Scholar 

  11. Luyt C-E, Hékimian G, Bonnet I et al (2020) Usefulness of point-of-care multiplex PCR to rapidly identify pathogens responsible for ventilator-associated pneumonia and their resistance to antibiotics: an observational study. Crit Care 24:378. https://doi.org/10.1186/s13054-020-03102-2

    Article  PubMed  PubMed Central  Google Scholar 

  12. Peiffer-Smadja N, Bouadma L, Mathy V et al (2020) Performance and impact of a multiplex PCR in ICU patients with ventilator-associated pneumonia or ventilated hospital-acquired pneumonia. Crit Care 24:366. https://doi.org/10.1186/s13054-020-03067-2

    Article  PubMed  PubMed Central  Google Scholar 

  13. Enne VI, Aydin A, Baldan R et al (2022) Multicentre evaluation of two multiplex PCR platforms for the rapid microbiological investigation of nosocomial pneumonia in UK ICUs: the INHALE WP1 study. Thorax. https://doi.org/10.1136/thoraxjnl-2021-216990

    Article  PubMed  Google Scholar 

  14. Navapurkar V, Bartholdson Scott J, Maes M et al (2021) Development and implementation of a customised rapid syndromic diagnostic test for severe pneumonia. Wellcome Open Res 6:256. https://doi.org/10.12688/wellcomeopenres.17099.1

    Article  CAS  Google Scholar 

  15. Moradi T, Bennett N, Shemanski S et al (2020) Use of procalcitonin and a respiratory polymerase chain reaction panel to reduce antibiotic use via an electronic medical record alert. Clin Infect Dis 71:1684–1689. https://doi.org/10.1093/cid/ciz1042

    Article  PubMed  Google Scholar 

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Acknowledgements

ACM is supported by a Medical Research Council Clinician Scientist Fellowship (MR/V006118/1). The authors wish to acknowledge Pedro Povoa who had the original idea for the article.

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Authors and Affiliations

  1. Division of Immunology, Department of Pathology, University of Cambridge, Cambridge, UK

    Andrew Conway Morris

  2. Division of Anaesthesia, Department of Medicine, University of Cambridge, Cambridge, UK

    Andrew Conway Morris

  3. JVF Intensive Care Unit, Addenbrooke’s Hospital, Cambridge, UK

    Andrew Conway Morris

  4. Intensive Care, Amsterdam UMC-Location AMC, University of Amsterdam, Amsterdam, The Netherlands

    Lieuwe D. J. Bos

  5. Médecine Intensive-Réanimation, Hôpital R. Salengro, CHU de Lille, 59000, Lille, France

    Saad Nseir

  6. Inserm U1285, Université de Lille, CNRS, UMR 8576-UGSF, 59000, Lille, France

    Saad Nseir

Authors
  1. Andrew Conway Morris
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  2. Lieuwe D. J. Bos
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  3. Saad Nseir
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Contributions

ACM, LDJB and SN conceived the article, generated the outline, wrote the first draft, critically revised the manuscript for important intellectual content, and agreed the final submitted version of the manuscript.

Corresponding author

Correspondence to Saad Nseir.

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Conflicts of interest

ACM has received payment for speaking on behalf of Boston Scientific and sits on the Scientific Advisory Board of Cambridge Infection Diagnostics, a start-up seeking to develop novel diagnostics for infectious diseases. SN received payment for lectures from MSD, Pfizer, Gilead, Biomérieux, Bio Rad, and Fischer and Paykel.

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Conway Morris, A., Bos, L.D.J. & Nseir, S. Molecular diagnostics in severe pneumonia: a new dawn or false promise?. Intensive Care Med 48, 740–742 (2022). https://doi.org/10.1007/s00134-022-06722-0

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