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The human response to infection is associated with distinct patterns of interleukin 23 and interleukin 27 expression

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Abstract

Objective

The development and progression of severe sepsis is related to a deficiency in pro-inflammatory cytokine production, characterised by lesser IFNγ levels, which are not explained by variations in levels of the main putative regulator of IFNγ, namely IL-12. As alternative regulators of IFNγ may be of greater importance in human sepsis, we investigated the hypothesis that the development of severe sepsis is related to variations in IL-18, IL-23 and IL-27 gene expression.

Design and setting

A prospective observational trial in a mixed intensive care unit (ICU) and hospital wards in a university teaching hospital.

Patients and participants

Sixty-two ICU patients with severe sepsis, 13 bacteraemic patients with no acute critical illness, and 10 healthy controls.

Measurements and results

All subjects were assayed for IL-18, IL-23 and IL-27 mRNA levels in peripheral blood. IL-27 mRNA levels distinguished between the three groups, with levels highest in the ICU group, intermediate in the bacteraemic group and lowest in the control group. IL-23 distinguished between the groups, with levels lowest in the ICU group. In late sepsis IL-23 and TNFα mRNA levels were directly related. IL-18 mRNA levels did not distinguish between the patient groups.

Conclusions

We conclude that the deficient pro-inflammatory response in patients with sepsis is expansive and includes deficient IL-23 and excessive IL-27 gene expression. This provides further evidence that upregulation of a cytokine-based immune response is beneficial in sepsis.

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Acknowledgements

Dr. Ross McManus is a Wellcome Trust/Health Research Board lecturer. We wish to acknowledge funding from the Higher Education Authority Programme for Research in Third Level Institutions.

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Correspondence to Thomas Ryan.

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Dr. Ryan and Dr. McManus are joint senior authors.

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O’Dwyer, M.J., Mankan, A.K., White, M. et al. The human response to infection is associated with distinct patterns of interleukin 23 and interleukin 27 expression. Intensive Care Med 34, 683–691 (2008). https://doi.org/10.1007/s00134-007-0968-5

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  • DOI: https://doi.org/10.1007/s00134-007-0968-5

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