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Effects of polyenylphosphatidylcholine on cytokines, nitrite/nitrate levels, antioxidant activity and lipid peroxidation in rats with sepsis

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Abstract

Objectives

To determine the effect of pretreatment with polyenylphosphatidylcholine (lecithin, PPC) on plasma levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, total nitrite/nitrate (NOx), and tissue levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in septic rats.

Design

Prospective, randomized, controlled animal study.

Setting

University laboratory.

Subjects

Forty-five Spraque-Dawley rats were divided into three groups: group C, sham-operated; group S, sepsis; and group P, sepsis pretreated with PPC.

Interventions

Rats were made septic by cecal ligation and puncture (CLP). Group P rats were treated with PPC (100 mg/day orally) for 10 days before sepsis. Twenty-four hours later CLP, plasma concentrations of TNF-α, IL-6 and IL-10 and plasma levels of NOx were measured. SOD and MDA were determined in liver, lung and heart homogenates.

Measurements and main results

All rats in group P survived during the 24-h observation time after CLP, whereas survival rate in group S was 66.7% (10/15; P<0.05). PPC significantly reduced plasma levels of TNF-α (P=0.006), IL-6 (P=0.007), IL-10 (P=0.016), NOx (P<0.001), and tissue levels of MDA (P<0.001) in group P with respect to in group S. Tissue levels of SOD significantly increased in group P when compared with group S (P<0.001).

Conclusions

These results show that PPC pretreatment exerts cumulative effects in decreasing the levels of cytokines, NOx, and tissue MDA concentrations, with a concomitant increase in survival in septic rats. Lecithin therapy may be a useful adjuvant therapy in controlling of the excessive production of the inflammatory cytokines in patients with severe sepsis.

Descriptor

SIRS/sepsis, experimental studies

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Acknowledgement

This study was supported by grants from the Scientific Research Foundation of İnönü University (2002/04).

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Authors and Affiliations

  1. Department of Anesthesiology and Reanimation, Medical School of İnönü University, 44315, Malatya, Turkey

    Semra Demirbilek & Mehmet Özcan Ersoy

  2. Department of Pediatric Surgery, Medical School of İnönü University, Malatya, Turkey

    Savaş Demirbilek, Abdurrahman Karaman & Melih Akın

  3. Department of Microbiology, Medical School of İnönü University, Malatya, Turkey

    Mehmet Bayraktar

  4. Department of Biochemistry, Medical School of İnönü University, Malatya, Turkey

    Nihayet Bayraktar

Authors
  1. Semra Demirbilek
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  2. Mehmet Özcan Ersoy
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  3. Savaş Demirbilek
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  4. Abdurrahman Karaman
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  5. Melih Akın
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  6. Mehmet Bayraktar
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  7. Nihayet Bayraktar
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Correspondence to Semra Demirbilek.

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Demirbilek, S., Ersoy, M.Ö., Demirbilek, S. et al. Effects of polyenylphosphatidylcholine on cytokines, nitrite/nitrate levels, antioxidant activity and lipid peroxidation in rats with sepsis. Intensive Care Med 30, 1974–1978 (2004). https://doi.org/10.1007/s00134-004-2234-4

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  • DOI: https://doi.org/10.1007/s00134-004-2234-4

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