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Oxidative Stress in a Wild Population of the Freshwater Fish Hyphessobrycon Luetkenii Chronically Exposed to a Copper Mining Area: New Insights into Copper Toxicology

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Abstract

In this study, we examine markers of oxidative stress in the tetra Hyphessobrycon luetkenii collected from two locations in the copper contaminated João Dias creek (southern Brazil). Also, specimens were translocated from a clean reference section of the creek to a polluted stretch and vice-versa. Fish were held at in submerged cages for 96 h and then sacrificed. Nuclear abnormalities in erythrocytes and total antioxidant capacity, lipid peroxidation and protein carbonylation in gills, brain, liver and muscle displayed similar trends in both groups. Lipid peroxidation increased in all tissues of individuals translocated to the polluted site but only in liver and muscle of those translocated to the reference site. Increased protein carbonylation was also observed in gills of individuals translocated to the reference location. These results suggest similar oxidative stress among fish from the reference and polluted locations and that long-term metals exposure may require adaptations toward oxidative stress responses.

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Acknowledgements

Thanks to Dr. Sandra I. M. Abril for her help in the field experiment. The present study was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Instituto Nacional de Ciência e Tecnologia de Toxicologia Aquática, Brasília, DF, Brazil. grant #573949/2008-5) and the International Development Research Centre (IDRC, Ottawa, ON, Canada, grant #104519-027). A. Bianchini (Proc. # 311410/2021-9) is a research fellow from the Brazilian CNPq and was supported by the International Canada Research Chair Program (IDRC).

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Authors and Affiliations

  1. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Avenida Itália km 8, Campus Carreiros, 96.203-900, Rio Grande, Rio Grande, RS, Brazil

    Vinícius Dias Borges, Yuri Dornelles Zebral, Patrícia Gomes Costa, Juliana da Silva Fonseca, Roberta Daniele Klein & Adalto Bianchini

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  1. Vinícius Dias Borges
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  2. Yuri Dornelles Zebral
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  3. Patrícia Gomes Costa
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  4. Juliana da Silva Fonseca
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  5. Roberta Daniele Klein
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Correspondence to Adalto Bianchini.

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Borges, V.D., Zebral, Y.D., Costa, P.G. et al. Oxidative Stress in a Wild Population of the Freshwater Fish Hyphessobrycon Luetkenii Chronically Exposed to a Copper Mining Area: New Insights into Copper Toxicology. Bull Environ Contam Toxicol 110, 77 (2023). https://doi.org/10.1007/s00128-023-03721-9

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