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Acute Toxicity of a Novel anti-fouling Material Additive DCOIT to Marine Chlorella sp

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Abstract

DCOIT (4,5-dichloro-2-n-octyl-4-isothiazolin-3-one) is the main ingredient in SeaNine-211, a new antifouling agent that replaces organotin compounds to prevent the growth of fouling organisms on board. Biocides from antifoulants can cause problems for marine ecosystems by destroying non-target algal species. This study evaluated the potential adverse effects DCOIT using the Marine Chlorella sp. The concentration of DCOIT were set according to the semi-inhibitory concentrations for acute exposure experiments, and relevant oxidative stress indicators were measured to assess the acute toxic effects. The results showed that the inhibition concentrations (IC50) of DCOIT against Marine Chlorella sp was 2.522 mg/L. The genes related to photosynthesis and antioxidant capacity showed the effect of promoting low concentration and inhibiting high concentration. In addition, based on the ultrastructural observation and the expression analysis of photosynthesis related genes, it was found that DCOIT had a significant effect on plant photosynthesis.

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Acknowledgements

This work was financially supported by National Key R&D Program of China (2018YFD0900905, 2018YFD0900902).

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Authors and Affiliations

  1. Marine College, Shandong University, 264209, Weihai, Shandong, China

    Jin-Chuang Ru, Xue-Li Zhao, Zhi-Han Cao, Cheng-Zhuang Chen, Ping Li & Zhi-Hua Li

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  1. Jin-Chuang Ru
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  2. Xue-Li Zhao
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  3. Zhi-Han Cao
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  4. Cheng-Zhuang Chen
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  5. Ping Li
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  6. Zhi-Hua Li
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Correspondence to Ping Li or Zhi-Hua Li.

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Ru, JC., Zhao, XL., Cao, ZH. et al. Acute Toxicity of a Novel anti-fouling Material Additive DCOIT to Marine Chlorella sp. Bull Environ Contam Toxicol 109, 1018–1022 (2022). https://doi.org/10.1007/s00128-022-03623-2

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