Abstract
The toxicity of pesticides to non-target organisms continues to be important in understanding the dynamic interactions between anthropogenic chemicals and ecosystem health. This study assesses biochemical markers to determine the effects that varying concentrations of atrazine (13.1–5557 µg/l) have on the freshwater shrimp, Caridina africana. Exposure and oxidative stress biomarkers were analysed and followed by univariate, integrated biomarker response v2 (IBRv2) and Kendall Tau correlation statistical analyses, to gain insight into the concentration-dependent responses. Oxidative stress biomarkers such as reduced glutathione content (GSH), glutathione-S-transferase activity (GST), superoxide dismutase activity (SOD) and catalase activity (CAT) were significantly correlated with increasing atrazine exposure concentration (p < 0.01). Bimodality has been seen when looking at both the univariate statistically significant differences as well as the IBRv2, with the first peak at 106.8 µg/l and the second peak at 5557 µg/l atrazine. The results indicate that while individual responses may indicate statistically significant differences, using correlation and integrated statistical analysis can shed light on trends in the adaptive response of these.
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Acknowledgements
We would like to the National Research Foundation of South Africa for the financial support granted to GR Jansen van Rensburg (NRF scarce skills scholarship, Grant 101,325). We would also like to thank the University of Johannesburg and the Water Research Group (WRG) at the North-West University for their support during this study.
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van Rensburg, G.J., Wepener, V., Horn, S. et al. Oxidative stress in the freshwater shrimp Caridina africana following exposure to atrazine. Bull Environ Contam Toxicol 109, 443–449 (2022). https://doi.org/10.1007/s00128-022-03526-2
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DOI: https://doi.org/10.1007/s00128-022-03526-2