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Production of Hydroxyl Radicals from Oxygenation of Simulated Acid Mine Drainage in Presence of Extracellular Polymers Substances

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Abstract

The reaction mechanisms Fe(II) abiotic oxidation produce ·OH by CaCO3-induced in acid mine drainage (AMD) are well-documented, but little is known about the influence of extracellular polymeric substances (EPS) secreted by microorganisms on Fe(II) oxidation in AMD. In this study, ·OH production was experimently measured from oxygenation of simulated AMD in the presence of EPS. The cumulative ·OH increased from 56.75 to 158.70 µM within 24 h at pH 3 with the increase in EPS concentration from 0 to 12 mg/L. An appropriate pH (about 6) and EPS (6 mg/L) concentration were required for the moderate rate of Fe(II) oxidation. Besides, the yield of ·OH increased remarkably with the addition of Fe3+. In the presence of EPS, ·OH production is attributed mainly the complexation of Fe(II) with EPS, of which is rich of carboxyl and hydroxyl groups. The findings provide fundamental supplement of ·OH production from Fe(II) oxidation by microorganisms in natural AMD.

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Acknowledgements

This work was supported by the science and Technology Foundation of Guizhou Province (NO.(2018)1155), the Natural Science Foundation of China (NO.41763018), and the Program Foundation of Institute for Scientific Research of Karst Area of NSFC-GZGOV (NO.U1612442).

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Authors and Affiliations

  1. College of Resource and Environmental Engineering, Guizhou University, 550025, Guiyang, PR China

    Xuewen Zhong, Fang Liu, Pan Wu & Jian Zhu

  2. Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, 550025, Guiyang, PR China

    Fang Liu, Pan Wu & Jian Zhu

  3. Guizhou Institute of Soil and Fertilizer, 550006, Guiyang, PR China

    Xuewen Zhong & Feifei Fan

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  1. Xuewen Zhong
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  2. Fang Liu
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  3. Pan Wu
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  5. Jian Zhu
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Correspondence to Jian Zhu.

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Zhong, X., Liu, F., Wu, P. et al. Production of Hydroxyl Radicals from Oxygenation of Simulated Acid Mine Drainage in Presence of Extracellular Polymers Substances. Bull Environ Contam Toxicol 109, 771–775 (2022). https://doi.org/10.1007/s00128-022-03489-4

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