Abstract
In this study, the interactive effect of Cd and Pb on the growth of Capsicum annuum L. was studied through pot experiments, and the indicators of photosynthesis efficiency (PE) and antioxidant defense system (ADS) were measured at different plant ages. Single Pb stress on PE and ADS was stronger than single Cd stress at the first month. Both the PE and ADS response showed a significant decrease under the combined stress of Cd and Pb, which was primarily dependent on the Pb concentration. With increasing plant age, the PE and response of non-enzymatic ADS exhibited dramatic decreases under Cd and/or Pb stress, and the activities of enzymatic ADS showed increases to some extent. The factorial analysis showed that Cd and Pb had an interactive effect to reduce PE, while slightly enhanced the activities of enzymatic ADS. Those results are useful to explore the interaction between Cd and Pb in the combined stress and understand their accumulation in the plants.
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References
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126. https://doi.org/10.1016/S0076-6879(84)05016-3
Alamri SA, Siddiqui MH, Al-Khaishany MYY et al (2018) Ascorbic acid improves the tolerance of wheat plants to lead toxicity. J Plant Interact 13(1):409–419. https://doi.org/10.1080/17429145.2018.1491067
Bagheri M, Javanmard HR, Naderi MR (2021) Soil cadmium and lead affecting biochemical properties of Matricaria chamomilla L. at different growth stages in the greenhouse and field. Biometals. https://doi.org/10.1007/s10534-021-00314-z
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–208. https://doi.org/10.1007/BF00018060
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287. https://doi.org/10.1016/0003-2697(71)90370-8
Chance BA, Maehly C (1955) Assay of catalase and peroxidase. Methods Enzymol 2:764–775. https://doi.org/10.1016/S0076-6879(55)02300-8
Chapin FS, Autumn K, Pugntaire F (1993) Evolution of suites of traits in response to environmental stress. Am Nat 142:S78–S92. https://doi.org/10.1086/285524
Chen Q, Zhang X, Liu Y et al (2017) Hemin-mediated alleviation of zinc, lead and chromium toxicity is associated with elevated photosynthesis, antioxidative capacity; suppressed metal uptake and oxidative stress in rice seedlings. Plant Growth Regul 81(2):253–264. https://doi.org/10.1007/s10725-016-0202-y
Dias MC, Mariz-Pontec N, Santosc C (2019) Lead induces oxidative stress in Pisum sativum plants and changes the levels of phytohormones with antioxidant role. Plant Physiol Biochem 137:121–129. https://doi.org/10.1016/j.plaphy.2019.02.005
Fang Y, Sun X, Yang W et al (2014) Concentrations and health risks of lead, cadmium, arsenic, and mercury in rice and edible mushrooms in China. Food Chem 147:147–151. https://doi.org/10.1016/j.foodchem.2013.09.116
Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Bioch 48:909–930. https://doi.org/10.1016/j.plaphy.2010.08.016
Hayat S, Hayat Q, Alyemeni MN et al (2012) Role of proline under changing environments. Plant Signal Behav 7:1456–1466. https://doi.org/10.4161/psb.21949
Huang X, Jiang Y, Cheng X et al (2015) Photosynthetic performance and anti-oxidative response of Cornus controversa seedlings under cadmium and lead stress. Bangladesh J Bot 44(2):215–221. https://doi.org/10.3329/bjb.v44i2.38510
Khudsar T, Arshi A, Siddiqi TO et al (2008) Zinc-induced changes in growth characters, foliar properties, and Zn-accumulation capacity of pigeon pea at different stages of plant growth. J Plant Nutr 31:281–306. https://doi.org/10.1080/01904160701853894
Le YTT, Vijver MG, Kinraide TB et al (2013) Modelling metal interactions and metal toxicity to lettuce Lactuca sativa following mixture exposure (Cu2+-Zn2+ and Cu2+-Ag+). Environ Pollut 176:185–192. https://doi.org/10.1016/j.envpol.2013.01.017
Lin YF, Aarts MG (2012) The molecular mechanism of zinc and cadmium stress response in plants. Cell Mol Life Sci 69:3187–3206. https://doi.org/10.1007/s00018-012-1089-z
Liu J, Li N, Zhang W et al (2019) Thallium contamination in farmlands and common vegetables in a pyrite mining city and potential health risks. Environ Pollut 248:906–915. https://doi.org/10.1016/j.envpol.2019.02.092
Marrugo-Negrete J, Pinedo-Hernandez J, Diez S (2017) Assessment of heavy metal pollution, spatial distribution and origin in agricultural soils along the Sinu River Basin, Colombia. Environ Res 154:380–388. https://doi.org/10.1016/j.envres.2017.01.021
Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410. https://doi.org/10.1016/S1360-385(02)02312-9
Nagajyoti PC, Lee KD, Sreekanth TVM (2010) Heavy metals, occurrence and toxicity for plants: a review. Environ Chem Lett 8:199–216. https://doi.org/10.1007/s10311-010-0297-8
Pinto FR, Mourato MP, Sales JR et al (2017) Oxidative stress response in spinach plants induced by cadmium. J Plant Nutr 40(2):268–276. https://doi.org/10.1080/01904167.2016.1240186
Riaz M, Kamran M, Rizwan M et al (2021) Cadmium uptake and translocation: selenium and silicon roles in Cd detoxification for the production of low Cd crops: a critical review. Chemosphere 273:129690. https://doi.org/10.1016/j.chemosphere.2021.129690
Srivastava RK, Pandey P, Rajpoot R (2014) Cadmium and lead interactive effects on oxidative stress and antioxidative responses in rice seedlings. Protoplasma 251:1047–1065. https://doi.org/10.1007/s00709-014-0614-3
Wang J, Wang LL, Wang YX et al (2021) Emerging risks of toxic metal(loid)s in soil-vegetables influenced by steel-making activities and isotopic source apportionment. Environ Int 146:106207. https://doi.org/10.1016/j.envint.2020.106207
Xu Z, Zhou Q, Liu W (2009) Joint effects of cadmium and lead on seedlings of four Chinese cabbage cultivars in northeastern China. J Environ Sci 21(11):1598–1606. https://doi.org/10.1016/S1001-0742(08)62461-4
Yan J, Tsuichihara N, Etoh T et al (2007) Reactive oxygen species and nitric oxide are involved in ABA inhibition of stomatal opening. Plant Cell Environ 30:1320–1325. https://doi.org/10.1111/j.1365-3040.2007.01711.x
Zagorchev L, Seal CE, Kranner I et al (2013) A central role for thiols in plant tolerance to abiotic stress. Int J Mol Sci 14(4):7405–7432. https://doi.org/10.3390/ijms14047405
Zhang H, Li X, Xu Z et al (2020) Toxic effects of heavy metals Pb and Cd on mulberry (Morus alba L.) seedling leaves: photosynthetic function and reactive oxygen species (ROS) metabolism responses. Ecotoxicol Environ Saf. https://doi.org/10.1016/j.ecoenv.2020.110469
Zhou YT, Wang LL, Xiao TF et al (2020) Legacy of multiple heavy metal(loid)s contamination and ecological risks in farmland soils from a historical artisanal zinc smelting area. Sci Total Environ 720:137541. https://doi.org/10.1016/j.scitotenv.2020.137541
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It was supported by the National Natural Science Foundation of China (41877030) and the Post-Three Gorges Project Fund.
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Kou, M., Xiong, J., Li, M. et al. Interactive Effects of Cd and Pb on the Photosynthesis Efficiency and Antioxidant Defense System of Capsicum annuum L. Bull Environ Contam Toxicol 108, 917–925 (2022). https://doi.org/10.1007/s00128-021-03452-9
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DOI: https://doi.org/10.1007/s00128-021-03452-9