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Effects of Phenanthrene Stress on Gonads and Vitellogenin of Loach (Misgurnus anguillicaudatus)

Abstract

Phenanthrene (PHE) as a tricyclic polycyclic aromatic hydrocarbon is one of the common pollutants in water and sediments, which can cause reproductive toxicity to aquatic organisms. In this study, enzyme-linked immunosorbent assay (ELISA) was used to detect the vitellogenin (VTG) of loach, and then to explore the estrogenic toxicity effect of PHE on loach. The results were as follows: (1) with the increase of PHE concentrations and the extension of exposure time, the gonadosomatic index (GSI) of males decreased significantly, while it increased in female loaches. In addition, males had more obvious changes than females and were more sensitive to PHE. (2) The increase of VTG contents in serum of males were stronger than that in females. Those results reveal that PHE has estrogenic effect, which can affect the generation of VTG, thus causing damage to the gonad development of loach.

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Acknowledgements

This study is supported by the basic Research Program of Natural Science of Shaanxi Province (Grant No: 2012JM3012), the Project of benefiting the people of Science and Technology of Yan'an City (Grant No: 2017HM-05), the key disciplines of Ecology of Shaanxi Province, and the Innovation Program of Postgraduate Education of Yan'an University (Grant No: YCX201933).

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Correspondence to Xin Lei.

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Wu, H., An, P., Wang, J. et al. Effects of Phenanthrene Stress on Gonads and Vitellogenin of Loach (Misgurnus anguillicaudatus). Bull Environ Contam Toxicol 106, 289–294 (2021). https://doi.org/10.1007/s00128-020-03077-4

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Keywords

  • Phenanthrene
  • Loach
  • Vitellogenin
  • Estrogenic effect