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Effect of Graphene Oxide on Growth of Wheat Seedlings: Insights from Oxidative Stress and Physiological Flux

Abstract

In this study, the responses of wheat seedlings to graphene oxide (GO) were investigated at a wide concentration range of 0–1000 mg L−1, including oxidative stress, real-time membrane potential as well as proton and calcium ion fluxes. The results show that GO induced a hormesis effect on root growth (low concentration (100 mg L−1) promotion and high concentration (1000 mg L−1) inhibition. Oxidative stress was responsible for the growth inhibition at GO concentration of 1000 mg L−1, as suggested from great stimulation in the activities of antioxidant enzymes and MDA content in roots or leaves. Superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) activities were highly correlated with MDA levels (r2 = 0.963, 0.984, and 0.960, respectively). GO exposure caused significant concentration-dependent membrane depolarization in roots, and significantly inhibited H+ efflux and extracellular Ca2+ influx in root cap.

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Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (2017YFA0207001), the National Natural Science Foundation of China (Nos. 41877139, 41401565). The authors acknowledge the Xuyue Science & Technology Co. Ltd. for the scientific and technical assistance in Non-invasive Micro-Test.

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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by WR, HC and LL. The first draft of the manuscript was written by WR and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ying Teng.

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Ren, W., Chang, H., Li, L. et al. Effect of Graphene Oxide on Growth of Wheat Seedlings: Insights from Oxidative Stress and Physiological Flux. Bull Environ Contam Toxicol 105, 139–145 (2020). https://doi.org/10.1007/s00128-020-02888-9

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Keywords

  • Graphene oxide
  • Nanomaterial
  • Oxidative stress
  • Membrane potential
  • Ion flux