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Effect of Long-Term Pesticides and Chemical Fertilizers Application on the Microbial Community Specifically Anammox and Denitrifying Bacteria in Rice Field Soil of Jhenaidah and Kushtia District, Bangladesh

Abstract

In this study, we investigated the effect of long-term pesticides and chemical fertilizers application on the microbial communities specifically anammox and denitrification bacteria in rice field soils. The abundances of microbial communities (16S rDNA), anammox (hszB), and denitrification (narG, nirK, nirS, and nosZ) genes were quantified by q-PCR. 10 pesticides (5 insecticides, 3 fungicides and 2 herbicides) and chemical fertilizers urea, potassium, phosphate, DAP (di-ammonium phosphate), gypsum, and boric acid were used by local farmers. Nitrate, SOC (ammonia, soil organic carbon), N and C content significantly (p < 0.05) decreased in the rice field soils as compared to the upland soils. Abundance of 16S rDNA, hszB, narG, nirK, nirS, and nosZ genes significantly (p < 0.05) decreased in the rice field soils and positively correlated with chemical properties of soils. Our results provide useful information and further maintenance should be instilled to the potential of chemical and biological factors decreased in rice field soils.

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References

  1. Aktar MW, Sengupta D, Chowdhury A (2009) Impact of pesticides use in agriculture: their benefits and hazards. Interdisc Toxicol 2:1–12

    Article  Google Scholar 

  2. Ara I, Lewis M, Ostendorf B (2016) Spatio-temporal analysis of the impact of climate, cropping intensity and means of irrigation: an assessment on rice yield determinants in Bangladesh. Agric Food Secur 5:12. https://doi.org/10.1186/s40066-016-0061-9

    Article  Google Scholar 

  3. Bhattacharjee S, Fakhruddin ANM, Chowdhury MAZ et al (2012) Monitoring of selected pesticides residue levels in water samples of paddy fields and removal of cypermethrin and chlorpyrifos residues from water using rice bran. Bull Environ Contam Toxicol 89:348–353. https://doi.org/10.1007/s00128-012-0686-8

    CAS  Article  Google Scholar 

  4. Böhme L, Langer U, Böhme F (2005) Microbial biomass, enzyme activities and microbial community structure in two European long-term experiments. Agric Ecosyst Environ 109:141–152

    Article  Google Scholar 

  5. Bunemann EK, McNeill A (2004) Impact of fertilizers on soil biota. In: Lines R (ed) Proceedings current research into soil biology in agriculture. Kelly, Tamworth, pp 64–71

    Google Scholar 

  6. Chen Z, Luo X, Hu R, Wu M, Wu J, Wei W (2010) Impact of long-term fertilization on the composition of denitrifier communities based on nitrite reductase analyses in a paddy soil. Microbiol Ecol 60:850–861

    CAS  Article  Google Scholar 

  7. Cruz MK, Suttle KB, Brodie EL, Power ME, Andersen GL, Banfield JF (2009) Despite strong seasonal responses, soil microbial consortia are more resilient to long-term changes in rainfall than overlying grassland. ISME J 3:738–744

    Article  Google Scholar 

  8. Fabra A, Duffard R, Evangelista DDA (1997) Toxicity of 2,4-dichlorophenoxyacetic acid in pure culture. Bull Environ Contam Toxicol 59:645–652

    CAS  Article  Google Scholar 

  9. Giles ME, Morley NJ, Baggs EM, Daniell TJ (2012) Soil nitrate reducing processes-drivers, mechanisms for spatial variation and significance for nitrous oxide production. Front Microbiol 3:407

    CAS  Article  Google Scholar 

  10. Hao XH, Liu SL, Wu JS, Hu RG, Tong CL, Su YY (2008) Effect of long-term application of inorganic fertilizer and organic amendments on soil organic matter and microbial biomass in three subtropical paddy soils. Nutr Cycl Agroecosyst 81:17–24. https://doi.org/10.1007/s10705-007-9145-z

    Article  Google Scholar 

  11. Harhangi HR, Le Roy M, van Alen T, Hu BL, Groen J, Kartal B, Tringe SG, Quan ZX, Jetten MS, den Camp HJ (2012) Hydrazine synthase, a unique phylomarker with which to study the presence and biodiversity of anammox bacteria. Appl Environ Microbiol 78:752–758

    CAS  Article  Google Scholar 

  12. He Z, Xiao S, Xie X, Zhong H, Hu Y, Li Q, Gao F, Li G, Liu J, Qiu G (2007) Molecular diversity of microbial community in acid mine drainages of Yunfu sulfide mine. Extremophiles 11:305–314

    CAS  Article  Google Scholar 

  13. Henry S, Baudoin E, Lopez-Gutierrez JC, Martin-Laurent F, Braumanb A, Philippo L (2004) Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR. J Microbiol Methods 59:327–335

    CAS  Article  Google Scholar 

  14. Ishii S, Ohno H, Tsuboi M, Otsuka S, Senoo K (2011) Identification and isolation of active N2O reducers in rice paddy soil. ISME J 5:1936–1945

    CAS  Article  Google Scholar 

  15. Jetten MSM (2001) New pathways for ammonia conversion in soil and aquatic systems. Plant Soil 230:9–19

    CAS  Article  Google Scholar 

  16. Kandeler E, Deiglmayr K, Tscherko D, Bru D, Philippot L (2006) Abundance of narG, nirS, nirK, and nosZ gees of denitrifying bacteria during primary successions of a glacier foreland. Appl Environ Microbiol 72:5957–5962

    CAS  Article  Google Scholar 

  17. Kogel-Knabner I, Amelung W, Cao Z, Fiedler S, Frenzel P, Jahn R, Kalbitz K, Kolbl A, Schloter M (2010) Biogeochemistry of paddy soils. Geoderma 157:1–14

    Article  Google Scholar 

  18. Liu M, Li ZP, Zhang TL, Jiang CY, Che YP (2011) Discrepancy in response of rice yield and soil fertility to long-term chemical fertilization and organic amendments in paddy soils cultivated from infertile upland in subtropical China. Agr Sci China 10(2):259–266. https://doi.org/10.1016/S1671-2927(11)60003-5

    CAS  Article  Google Scholar 

  19. Lo CC (2010) Effect of pesticides on soil microbial community. J Environ Sci Health B 45:348–359

    CAS  Article  Google Scholar 

  20. Mainuddin M, Kirby M (2015) National food security in Bangladesh to 2050. Food Secur 7:633–646

    Article  Google Scholar 

  21. Martens DA, Bremner JM (1993) Influence of herbicides on transformations of urea nitrogen in soil. J Environ Sci Health B 28:377–395

    Article  Google Scholar 

  22. Meena RS, Kumar S, Datta R, Lal R, Vijayakumar V et al (2020) Impact of agrochemicals on soil microbiota and management: a review. Land 9:34. https://doi.org/10.3390/land9020034

    Article  Google Scholar 

  23. Nicomrat D, Tharajak J, Kanthang P (2016) Pesticides contaminated in rice paddy soil affecting on cultivated microorganism community. Appl Mech Mater 848:135–138. https://doi.org/10.4028/www.scientific.net/amm.848.135

    Article  Google Scholar 

  24. Pavel EW, Reneau RBJ, Berry DF, Smith EP, Mostaghimi S (1996) Denitrification potential of non-tidal riparian wetland soils in the Virginia coastal plain. Water Res 30:2798–2804

    CAS  Article  Google Scholar 

  25. Pell M, Stenberg B, Torstensson L (1998) Potential denitrification and nitrification tests for evaluation of pesticide effects in soil. Ambio 27(1):24–28

    Google Scholar 

  26. Peng S, Wang Y, Zhou B, Lin X (2015) Long-term application of fresh and composted manure increase tetracycline resistance in the arable soil of eastern China. Sci Total Environ 506:279–286

    Article  Google Scholar 

  27. Rahman MM, Shan J, Yang P, Shang X, Xia Y, Yan X (2018) Effects of long-term pig manure application on antibiotics, abundance of antibiotic resistance genes (ARGs), anammox and denitrification rates in paddy soils. Environ Pollut 240:368–377

    CAS  Article  Google Scholar 

  28. Saggar S, Jha N, Deslippe J, Bolan NS, Luo J, Giltrap DL, Kim DG, Zaman M, Tillman RW (2013) Denitrification and N2O:N2 production in temperate grasslands: processes, measurements, modelling and mitigating negative impacts. Sci Total Environ 465:173–195

    CAS  Article  Google Scholar 

  29. Santos A, Flores M (1995) Effects of glyphosate on nitrogen fixation of free-living heterotrophic bacteria. Lett Appl Microbiol 20:349–352

    CAS  Article  Google Scholar 

  30. Shan J, Zhao X, Sheng R, Xia Y, Ti C, Quan X, Wang S, Wei W, Yan X (2016) Dissimilatory nitrate reduction processes in typical Chinese paddy soils: rates, relative contributions and influencing factors. Environ Sci Technol 50:9972–9980

    CAS  Article  Google Scholar 

  31. Shen LD, Wu HS, Gao ZQ, Xu XH, Chen TX, Liu S, Cheng HX (2015) Occurrence and importance of anaerobic ammonium-oxidizing bacteria in vegetable soils. Appl Microbiol Biotechnol 99:5709–5718

    CAS  Article  Google Scholar 

  32. Sheng R, Meng DL, Wu MN, Di HJ, Qin HL, Wei WX (2013) Effect of agricultural land use change on community composition of bacteria and ammonia oxidizers. J Soils Sedim 13:1246–1256

    Article  Google Scholar 

  33. Thamdrup B, Dalsgaard T (2002) Production of N2 through anaerobic ammonium oxidation coupled to nitrate reduction in marine sediments. Appl Environ Microbiol 68:1312–1318

    CAS  Article  Google Scholar 

  34. Throback IN, Enwall K, Jarvis A, Hallin S (2004) Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE. FEMS Microbiol Ecol 49:401–417

    CAS  Article  Google Scholar 

  35. Xu YB, Xu ZH, Cai ZC, Reverchon F (2013) Review of denitrification in tropical and subtropical soils of terrestrial ecosystems. J Soils Sedim 13:699–710

    CAS  Article  Google Scholar 

  36. Yang XR, Li H, Nie SA, Su JQ, Weng BS, Zhu GB, Yao HY, Gilbert JA, Zhu YG (2015) The potential contribution of anammox to nitrogen loss from paddy soils in Southern China. Appl Environ Microbiol 81:938–947

    Article  Google Scholar 

  37. Yoshida M, Ishii S, Otsuka S, Senoo K (2009) Temporal shifts in diversity and quantity of nirS and nirK in a rice paddy field soil. Soil Biol Biochem 41:2044–2051

    CAS  Article  Google Scholar 

  38. Zhao Y, Xia Y, Ti C, Shan J, Li B, Xia L, Yan X (2015) Nitrogen removal capacity of the river network in a high nitrogen loading region. Environ Sci Technol 49:1427–1435

    CAS  Article  Google Scholar 

  39. Zheng Y, Zhang LM, Zheng YM, Di H, He JZ (2008) Abundance and community composition of methanotrophs in a Chinese paddy soil under long-term fertilization practices. J Soils Sediments 8:406–414

    CAS  Article  Google Scholar 

Download references

Acknowledgements

The authors are grateful to Prodip Kumar Adhikary, Assistant Professor, Department of English, Islamic University, Kushtia-7003, Bangladesh for checking the English languages and grammatical error. The authors are thankful to the Department of Biotechnology and Genetic engineering, Islamic University, Kushtia-7003, Bangladesh for their cordial support to carry out this research. Authors are also grateful to the local farmers for supporting the collection of data.

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Correspondence to M. Mizanur Rahman.

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Rahman, M.M., Nahar, K., Ali, M.M. et al. Effect of Long-Term Pesticides and Chemical Fertilizers Application on the Microbial Community Specifically Anammox and Denitrifying Bacteria in Rice Field Soil of Jhenaidah and Kushtia District, Bangladesh. Bull Environ Contam Toxicol 104, 828–833 (2020). https://doi.org/10.1007/s00128-020-02870-5

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Keywords

  • Long-term
  • Pesticides
  • Chemical fertilizers
  • Microbial communities
  • Anammox and denitrifying bacteria
  • Rice field soil