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Respiratory Toxicity of Azoxystrobin, Pyraclostrobin and Coumoxystrobin on Chlorella vulgaris

Abstract

Azoxystrobin (AZ), pyraclostrobin (PYR) and coumoxystrobin (COU) exert negative impacts on Chlorella vulgaris. Thus, in this study, C. vulgaris was used to assess the respiratory toxicity of AZ, PYR and COU by determining the acute toxicity, complex III activity and ATP viability. The 96 h-EC50 values of AZ, PYR and COU for C. vulgaris were 1.85, 2.21 and 1.62 mg/L, respectively. AZ, PYR and COU exerted significant effects on complex III activity and ATP viability after exposure to 0.71, 1.01 and 1.08 mg/L of the fungicides. The binding potentials of AZ, PYR and COU toward ubiquinone were − 10.44, − 9.31 and − 12.98 kcal/mol, respectively, which had adverse effects on amino acids. These results provided new insight into the potential acute respiratory toxicity mechanisms of these strobilurin fungicides in algae.

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References

  1. Ban K, Kozar RA (2011) Glutamine protects against apoptosis via downregulation of Sp3 in intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol 299(6):G1344–G1353

    Article  Google Scholar 

  2. Berenzen N, Lentzen-Godding A, Probst M, Schulz H, Schulz R, Liess M (2005) A comparison of predicted and measured levels of runoff-related pesticide concentrations in small lowland streams on a landscape level. Chemistry 58:683–691

    CAS  Google Scholar 

  3. Cavalcante AAM, de Vasconcelos PRL (2015) Enteral nutrition supplemented with l-glutamine in patients with sepsis. In: Rajendram R, Preedy V, Patel V (eds) Glutamine in clinical nutrition. Nutrition and health. Humana Press, New York, NY, pp 169–179

    Chapter  Google Scholar 

  4. Cui F, Chai T, Liu X, Wang C (2017) Toxicity of three strobilurins (kresoxim-methyl, pyraclostrobin, and trifloxystrobin) on Daphnia magna. Environ Toxicol Chem 36:182–189

    CAS  Article  Google Scholar 

  5. Esser L, Quinn B, Li F, Zhang M, Elberry M, Yu L, Yu C, Xia D (2004) Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex. J Mol Biol 341:281–302

    CAS  Article  Google Scholar 

  6. Fernández-Checa JC, Kaplowitz N, García-Ruiz C, Colell A (1998) Mitochondrial glutathione: importance and transport. Semin Liver Dis 18:389–401

    Article  Google Scholar 

  7. Gao AH, Fu YY, Zhang KZ, Zhang M, Fan L, Nan F, Yuan C, Li J, Li J (2014) Azoxystrobin, a mitochondrial complex III Qo site inhibitor, exerts beneficial metabolic effects in vivo and in vitro. Biochim Biophys Acta 1840:2212–2221

    CAS  Article  Google Scholar 

  8. Hnatova MG, Obernauerova M, Subikova V, Subik J (2003) Cross-resistance to strobilurin fungicides in mitochondrial and nuclear mutants of Saccharomyces cerevisiae. Folia Microbiol 48(4):496–500

    CAS  Article  Google Scholar 

  9. Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M (1998) Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci Biotechnol Biochem 62:145–147

    CAS  Article  Google Scholar 

  10. Leist M, Single B, Castoldi AF, Kuhnle S, Nicotera P (1997) Intracellular adenosine triphosphate (ATP) concentration: a switch in the decision between apoptosis and necrosis. J Exp Med 185:1481–1486

    CAS  Article  Google Scholar 

  11. Li H, Liu M, Yang Y, Shi H, Zhou J, He D (2018) Developmental toxicity, oxidative stress and immunotoxicity induced by three strobilurins (pyraclostrobin, trifloxystrobin and picoxystrobin) in zebrafish embryos. Chemistry 207:781–790

    CAS  Google Scholar 

  12. Liess M (2005) Analyzing effects of pesticides on invertebrate communities in streams. Environ Toxicol Chem 24:954–965

    CAS  Article  Google Scholar 

  13. Liu X, Wang Y, Chen H, Zhang J, Wang C, Li X, Pang S (2018) Acute toxicity and associated mechanisms of four strobilurins in algae. Environ Toxicol Pharmacol 60:12–16

    CAS  Article  Google Scholar 

  14. Lu B (2018) Azoxystrobin and pyraclostrobin dominate the market of Strobilurin fungicides. Shangdong Pesticide News 4:24–30

    Google Scholar 

  15. Lu T, Zhu Y, Xu J (2018) Evaluation of the toxic response induced by azoxystrobin in the non-target green alga Chlorella pyrenoidosa. Environ Pollut 234:379–388

    CAS  Article  Google Scholar 

  16. Lv X, Pan L, Wang J (2017) Effects of triazole fungicides on androgenic disruption and CYP3A4 enzyme activity. Environ Pollut 222:504–512

    CAS  Article  Google Scholar 

  17. Ochoa-Acuna HG, Bialkowski W, Gowri Y, Hahn L (2009) Toxicity of soybean rust fungicides to freshwater algae and Daphnia magna. Ecotoxicology 18:440–446

    CAS  Article  Google Scholar 

  18. Qi S, Wang C (2013) Toxicity assessments with Daphnia magna of Guadipyr, a new neonicotinoid insecticide and studies of its effect on acetylcholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT) and chitobiaseactivities. Ecotoxicol Environ Saf 98:339–344

    CAS  Article  Google Scholar 

  19. Reilly TJ, Smalling K, Orlando J, Kuivila K (2012) Occurrence of boscalid and other selected fungicides in surface water and groundwater in three targeted use areas in the United States. Chemistry 89:228–234

    CAS  Google Scholar 

  20. Rodrigues ET, Lopes I, Pardal MA (2013) Occurrence, fate and effects of azoxystrobin in aquatic ecosystems: a review. Environ Int 53:18–28

    CAS  Article  Google Scholar 

  21. SAC (2017) Chinese National Standard ICS 65.020.01.14-2017 of test guideline on environmental safety assessment for chemical pesticides

  22. Teodoro G, Aylin A, Erdemli O (2011) The effect of L-alanyl-L-glutamine dipeptide supplemented total parenteral nutrition on infectious morbidity and insulin sensitivity in critically ill patients. Crit Care Med 39:1263–1268

    Article  Google Scholar 

  23. Wightwick AM, Bui A, Zhang P, Gavin R (2012) Environmental fate of fungicides in surface waters of a horticultural-production catchment in southeastern Australia. Arch Environ Contam Toxicol 62:380–390

    CAS  Article  Google Scholar 

  24. Willke T (2014) Methionine production – a critical review. Appl Microbiol Biotechnol 98:9893–9914

    CAS  Article  Google Scholar 

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Acknowledgements

The project of this research is supported by the Key Projects funds from Liaoning Education Department (Serial Number: 01032017004).

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Correspondence to Kai Wang.

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Wang, K., Sun, Z., Yang, L. et al. Respiratory Toxicity of Azoxystrobin, Pyraclostrobin and Coumoxystrobin on Chlorella vulgaris. Bull Environ Contam Toxicol 104, 799–803 (2020). https://doi.org/10.1007/s00128-020-02869-y

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Keywords

  • Respiratory toxicity
  • Strobilurin fungicides
  • Chlorella vulgaris