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Biomarkers of Planarian Dugesia japonica in Response to Herbicide Glyphosate Exposure

Abstract

As the worldwide top-selling herbicide, glyphosate is ubiquitously distributed in the natural environment, and its influence on the ecological safety and human health has being increasingly concerned. In this study, mRNA expressions of GPX and three heat shock protein genes in freshwater planarian Dugesia japonica in response to glyphosate were determined, and two oxidative stress parameters were measured. The results suggested that GPX activity can be used as a more sensitive biomarker in contrast with GPX gene expression, and mRNA expressions of Hsp70, Hsp90 genes are more sensitive than Hsp40 for planarians in response to glyphosate stress. Besides, the deduced T-AOC as well as varied GPX activity and mRNA expression levels of Hsps also indicated that glyphosate exposure would inhibit antioxidation and induce oxidative stress in D. japonica, while specific antioxidant systems and stress proteins tried to protect cells by their own regulation. The results of this study will be helpful to elucidate the stress response mechanisms of freshwater planarians to herbicide glyphosate.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 31702010 and 31471965).

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Correspondence to Guang-Wen Chen.

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Zhang, HC., Shi, CY., Yang, YJ. et al. Biomarkers of Planarian Dugesia japonica in Response to Herbicide Glyphosate Exposure. Bull Environ Contam Toxicol 104, 804–808 (2020). https://doi.org/10.1007/s00128-020-02865-2

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Keywords

  • Freshwater planarian
  • Biomarker
  • Oxidative stress
  • Heat shock protein