Skip to main content

Correct Fish Muscle AChE Extraction for Use in Environmental Risk Assessment and Ecotoxicological Studies Has Been Neglected

Abstract

Several articles published in the field of ecotoxicology and environmental risk assessment have failed to adequately use fish muscle cholinesterases as biomarkers. This letter seeks to underline the importance of developing and applying correct protocols for the extraction of these enzymes from the tissues of these animals.

This is a preview of subscription content, access via your institution.

References

  1. de Oliveira FG et al (2019) Toxicological effects of anthropogenic activities in Geophagus brasiliensis from a coastal river of southern Brazil: a biomarker approach. Sci Total Environ 667:371–383

    Article  Google Scholar 

  2. Duncan WP et al (2020) Acute toxicity of the pesticide trichlorfon and inhibition of acetylcholinesterase in Colossoma macropomum (Characiformes: Serrasalmidae). Aquacult Int 28:815–830

    CAS  Article  Google Scholar 

  3. Lopes RM et al (2018) The importance of an efficient extraction protocol for the use of fish muscle cholinesterases as biomarkers. Talanta 179:769–774

    CAS  Article  Google Scholar 

  4. Martinez-Morcillo S et al (2019) Comparative study of esterase activities in different tissues of marine fish species Trachurus trachurus, Merluccius merluccius and Trisopterus luscus. Sci Total Environ 679:12–22

    CAS  Article  Google Scholar 

  5. Massoulie J, Bon S (1982) The molecular-forms of cholinesterase and acetylcholinesterase in vertebrates. Annu Rev Neurosci 5:57–106

    CAS  Article  Google Scholar 

  6. Massoulie J et al (1998) Acetylcholinesterase: C-terminal domains, molecular forms and functional localization. J Physiol-Paris 92:183–190

    CAS  Article  Google Scholar 

  7. Pereira BVR et al (2019) Cholinesterases characterization of three tropical fish species, and their sensitivity towards specific contaminants. Ecotoxicol Environ Saf 173:482–493

    CAS  Article  Google Scholar 

  8. Tesser ME et al (2020) Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach. Sci Total Environ 704:12

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Renato Matos Lopes.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Lopes, R.M. Correct Fish Muscle AChE Extraction for Use in Environmental Risk Assessment and Ecotoxicological Studies Has Been Neglected. Bull Environ Contam Toxicol 104, 721–722 (2020). https://doi.org/10.1007/s00128-020-02859-0

Download citation

Keywords

  • Acetylcholinesterase
  • Muscle
  • Fish
  • Ecotoxicology
  • Environmental risk assessment