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Genotoxic Effects of Environmental Pollutant Heavy Metals on Alburnus chalcoides (Pisces: Cyprinidae) Inhabiting Lower Melet River (Ordu, Turkey)

Abstract

The lower Melet River is a drinking water source that is surrounded by hazelnut grove, agricultural lands, resulting in the accumulation of genotoxic agents such as mining activities, various domestic and agricultural wastes. Therefore, it receives many domestic and agricultural wastes that contain the genotoxic agent. This study was aimed to assess the heavy metal concentrations in water, sediment, and bioaccumulation in the tissues of Alburnus chalcoides. Comet assay and micronucleus test were used to evaluate the genotoxic effects on the blood cells of A. chalcoides. The concentrations of heavy metals and metalloid in the water, in the sediments and in the muscle of fish were in the order of Fe > Al > Mn > As > Zn > Cu > Ni > Cr > Cd = Pb = Co, Fe > Al > Mn > Zn > Cu > Pb > Cr > As > Co > Ni > Cd and Fe > Zn > Al > Mn > Cu > Pb > As > Cr > Ni > Co > Cd, respectively. The blood cells of fish collected from the polluted location showed significantly higher DNA damage and micronucleus frequency compared to the reference location (p < 0.05). The study indicated that the DNA integrity of A. chalcoides was affected by heavy metals which originated from many anthropogenic sources.

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Acknowledgements

This study is based on part of the PhD thesis by Seda KONTAŞ at Ordu University. The study was supported by the Scientific Research Projects Commission of Ordu University (BAP; TF-1612). The corresponding author would as to thank TUBITAK-BIDEB within the scope of the 2211-E National Scholarship Program for PhD students.

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Correspondence to Seda Kontaş.

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Kontaş, S., Bostancı, D. Genotoxic Effects of Environmental Pollutant Heavy Metals on Alburnus chalcoides (Pisces: Cyprinidae) Inhabiting Lower Melet River (Ordu, Turkey). Bull Environ Contam Toxicol 104, 763–769 (2020). https://doi.org/10.1007/s00128-020-02857-2

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Keywords

  • Bioaccumulation
  • Comet assay
  • DNA damage
  • Ecotoxicology
  • Micronucleus test