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Evaluation of Cytotoxicity and Mutagenicity of Wastewater from Istanbul: Data from Hospitals and Advanced Wastewater Treatment Plant


Wastewater (WW) carry considerable amount of chemicals that could have mutagenic or cytotoxic effect from hospital discharges to aquatic environment. Our objective was to determinate the possible mutagenic and toxic effects of hospital originated WWs and effectiveness of the wastewater treatment plants (WTP) functions. In the study the mutagenic and cytotoxic potential of three hospitals and influent/effluent of a treatment plant WW collected in Istanbul and was examined using AMES, XTT, and lactate dehydrogenase (LDH) assays. Mutagenic effects were detected at both hospital discharges and advanced biological wastewater plant. We observed no cytotoxic effect in fibroblasts for LDH and XTT assays whereas high cytotoxicity for all samples was found in hepatocytes by XTT assay. According to the results even if advanced technology is used for treatment of WW, mutagenic and cytotoxic effects still remain, and the present technologies need to be further improved.

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We thank Istanbul Water And Sewerage Administration (ISKI) to permit to occur this study. This work was supported by the Marmara University Scientific Research Projects Commission (Project No: FEN-B-200716-0375). We thank Didem KUTLU, Ece CARPAR, Miray KARABAY and Elifcan GEZER for their contributions.

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Correspondence to Belgin Susleyici.

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Cevik, M., Dartan, G., Ulker, M. et al. Evaluation of Cytotoxicity and Mutagenicity of Wastewater from Istanbul: Data from Hospitals and Advanced Wastewater Treatment Plant. Bull Environ Contam Toxicol 104, 852–857 (2020).

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  • Wastewater
  • Hospital wastewater
  • Wastewater treatment plant
  • Mutagenicity
  • Cytotoxicity