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Evaluation of Cytotoxicity and Mutagenicity of Wastewater from Istanbul: Data from Hospitals and Advanced Wastewater Treatment Plant

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Abstract

Wastewater (WW) carry considerable amount of chemicals that could have mutagenic or cytotoxic effect from hospital discharges to aquatic environment. Our objective was to determinate the possible mutagenic and toxic effects of hospital originated WWs and effectiveness of the wastewater treatment plants (WTP) functions. In the study the mutagenic and cytotoxic potential of three hospitals and influent/effluent of a treatment plant WW collected in Istanbul and was examined using AMES, XTT, and lactate dehydrogenase (LDH) assays. Mutagenic effects were detected at both hospital discharges and advanced biological wastewater plant. We observed no cytotoxic effect in fibroblasts for LDH and XTT assays whereas high cytotoxicity for all samples was found in hepatocytes by XTT assay. According to the results even if advanced technology is used for treatment of WW, mutagenic and cytotoxic effects still remain, and the present technologies need to be further improved.

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Acknowledgements

We thank Istanbul Water And Sewerage Administration (ISKI) to permit to occur this study. This work was supported by the Marmara University Scientific Research Projects Commission (Project No: FEN-B-200716-0375). We thank Didem KUTLU, Ece CARPAR, Miray KARABAY and Elifcan GEZER for their contributions.

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Author notes

  1. Guler Dartan and Mehtap Cevik contributed equally to this study.

Authors and Affiliations

  1. Division of Biology, Department of Molecular Biology, Faculty of Arts and Sciences, Marmara University, Istanbul, Turkey

    Mehtap Cevik, Melike Ulker, Kıvanç Bezci & Belgin Susleyici

  2. Division of Chemistry, Department of Analytical Chemistry, Faculty of Arts and Sciences, Marmara University, Istanbul, Turkey

    Guler Dartan

  3. Division of Statistics, Faculty of Arts and Sciences, Marmara University, Istanbul, Turkey

    Gokce Deliorman

  4. Department of Medical Services and Technics, Vocational School of Health Service, Istanbul University - Cerrahpasa, Istanbul, Turkey

    Penbe Cagatay

  5. Department of Thoracic Surgery, Faculty of Medicine, Marmara University, Istanbul, Turkey

    Tunc Lacin

  6. Department of Anesthesiology and Intensive Care, Faculty of Medicine, Marmara University, Istanbul, Turkey

    Ismail Hakkı Cinel

  7. Department of Medical Microbiology, Faculty of Medicine, Marmara University, Istanbul, Turkey

    Burak Aksu

  8. Department of Public Health, Faculty of Medicine, Marmara University, Istanbul, Turkey

    Yasar Keskin

  9. Department of Environmental Engineering, Marmara University, Istanbul, Turkey

    Zehra Semra Can

  10. Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Marmara University, Istanbul, Turkey

    Turkan Yurdun

Authors
  1. Mehtap Cevik
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  2. Guler Dartan
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  3. Melike Ulker
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  4. Kıvanç Bezci
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  13. Belgin Susleyici
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Correspondence to Belgin Susleyici.

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Cevik, M., Dartan, G., Ulker, M. et al. Evaluation of Cytotoxicity and Mutagenicity of Wastewater from Istanbul: Data from Hospitals and Advanced Wastewater Treatment Plant. Bull Environ Contam Toxicol 104, 852–857 (2020). https://doi.org/10.1007/s00128-020-02853-6

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  • DOI: https://doi.org/10.1007/s00128-020-02853-6

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