The interaction between metal oxide nanoparticles and toxin-producing cyanobacteria is relatively unknown. The present work exposed Microcystis sp.7806 to different concentrations of cerium oxide nanoparticles (CeO2 NPs) (1 mg/L, 10 mg/L and 50 mg/L), and evaluated the growth, photosynthetic activity, reactive oxygen species level, and the extra-(intra-) cellular microcystin-LR (MC-LR) contents. The particle size, zeta potential and cerium ions released into the medium were analyzed. Results showed 10 mg/L NP treatment promoted algae growth but slightly inhibited the photosynthetic yield of algae, and the 50 mg/L treatment reduced algae biomass. The algal cells remarkably responded to oxidative stress at higher concentrations (10 mg/L and 50 mg/L). CeO2 NPs largely increased the intracellular MC-LR content at 50 mg/L, and significantly reduced the extracellular MC-LR content at any concentration. This demonstrates CeO2 NPs may pose an ecological risk potential during harmful algal blooms by stimulating toxin production.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price includes VAT (USA)
Tax calculation will be finalised during checkout.
Angel BM, Vallotton P, Apte SC (2015) On the mechanism of nanoparticulate CeO2 toxicity to freshwater algae. Aquat Toxicol 168:90–97
Bi X, Dai W, Zhou Q, Wang Y, Dong S, Zhang S, Qiao X, Zhu G (2016) Effect of anthracene (ANT) on growth, microcystin (MC) production and expression of MC synthetase (mcy) genes in Microcystis aeruginosa. Water Air Soil Pollut 227:259–266
Cao Q, Steinman AD, Wan X, Xie L (2018) Bioaccumulation of microcystin congeners in soil-plant system and human health risk assessment: a field study from Lake Taihu region of China. Environ Pollut 240:44–50
Chen L, Mao F, Kirumba GC, Jiang C, Manefield M, He Y (2015) Changes in metabolites, antioxidant system, and gene expression in Microcystis aeruginosa under sodium chloride stress. Ecotox Environ Safe 122:126–135
Dalai S, Pakrashi S, Bhuvaneshwari M, Iswarya V, Chandrasekaran N, Mukherjee A (2014) Toxic effect of Cr(VI) in presence of n-TiO2 and n-Al2O3 particles towards freshwater microalgae. Aquat Toxicol 146:28–37
Deng X-Y, Cheng J, Hu X-L, Wang L, Li D, Gao K (2017) Biological effects of TiO2 and CeO2 nanoparticles on the growth, photosynthetic activity, and cellular components of a marine diatom Phaeodactylum tricornutum. Sci Total Environ 575:87–96
Gagnon C, Bruneau A, Turcotte P, Pilote M, Gagné F (2018) Fate of cerium oxide nanoparticles in natural waters and immunotoxicity in exposed rainbow trout. J Nanomed Nanotechnol 9:8
Han J, Lee S, Choi K, Kim J, Ha D, Lee C-G, An B, Lee S-H, Mizuseki H, Choi J-W, Kang S (2016) Effect of nitrogen doping on titanium carbonitride-derived adsorbents used for arsenic removal. J Hazard Mater 302:375–385
Huang J, Cheng J, Yi J (2016) Impact of silver nanoparticles on marine diatom Skeletonema costatum. J Appl Toxicol 36:1343–1354
Huisman J, Codd GA, Paerl HW, Ibelings BW, Verspagen JMH, Visser PM (2018) Cyanobacterial blooms. Nat Rev Microbiol 16:471–483
Jain R, Jordan N, Weiss S, Foerstendorf H, Heim K, Kacker R, Hübner R, Kramer H, van Hullebusch ED, Farges F, Lens PNL (2015) Extracellular polymeric substances govern the surface charge of biogenic elemental selenium nanoparticles. Environ Sci Technol 49:1713–1720
Kroll A, Behra R, Kaegi R, Sigg L (2014) Extracellular polymeric substances (EPS) of freshwater biofilms stabilize and modify CeO2 and Ag nanoparticles. PLoS ONE 9:e110709
Leung Y, Yung MMN, Ng AMC, Ma APY, Wong SWY, Chan CMN, Ng Y, Djurisic AB, Guo M, Wong MT, Leung FCC, Chan WK, Leung KMY, Lee HK (2015) Toxicity of CeO2 nanoparticles-the effect of nanoparticle properties. J Photoch Photobio B 145:48–59
McLean TI (2013) "Eco-omics": a review of the application of genomics, transcriptomics, and proteomics for the study of the ecology of harmful algae. Microb Ecol 65:901–915
Melegari SP, Perreault F, Moukha S, Popovic R, Creppy EE, Matias WG (2012) Induction to oxidative stress by saxitoxin investigated through lipid peroxidation in neuro 2A cells and Chlamydomonas reinhardtii alga. Chemosphere 89:38–43
Middepogu A, Hou J, Gao X, Lin D (2018) Effect and mechanism of TiO2 nanoparticles on the photosynthesis of Chlorella pyrenoidosa. Ecotox Environ Safe 161:497–506
Mwaanga P, Carraway ER, van den Hurk P (2014) The induction of biochemical changes in Daphnia magna by CuO and ZnO nanoparticles. Aquat Toxicol 150:201–209
Ni L, Li D, Hu S, Wang P, Li S, Li Y, Li Y, Acharya K (2015) Effects of artemisinin sustained-release granules on mixed alga growth and microcystins production and release. Environ Sci Pollut R 22:18637–18644
Okupnik A, Contardo-Jara V, Pflugmacher S (2015) Potential role of engineered nanoparticles as contaminant carriers in aquatic ecosystems: Estimating sorption processes of the cyanobacterial toxin microcystin-LR by TiO2 nanoparticles. Colloids Surf A 481:460–467
Peng G, Martin RM, Dearth SP, Sun X, Boyer GL, Campagna SR, Lin S, Wilhelm SW (2018) Seasonally relevant cool temperatures interact with N chemistry to increase microcystins produced in lab cultures of Microcystis aeruginosa NIES-843. Environ Sci Technol 52:4127–4136
Röhder LA, Brandt T, Sigg L, Behra R (2014) Influence of agglomeration of cerium oxide nanoparticles and speciation of cerium(III) on short term effects to the green algae Chlamydomonas reinhardtii. Aquat Toxicol 152:121–130
Ranjith KS, Dong C-L, Lu Y-R, Huang Y-C, Chen C-L, Saravanan P, Asokan K, Rajendra Kumar RT (2018) Evolution of visible photocatalytic properties of Cu-doped CeO2 nanoparticles: Role of Cu2+-mediated oxygen vacancies and the mixed-valence states of Ce ions. ACS Sustain Chem Eng 6:8536–8546
Rodea-Palomares I, Boltes K, Fernandez-Pinas F, Leganes F, Garcia-Calvo E, Santiago J, Rosal R (2011) Physicochemical characterization and ecotoxicological assessment of CeO2 nanoparticles using two aquatic microorganisms. Toxicol Sci 119:135–145
Saison C, Perreault F, Daigle JC, Fortin C, Claverie J, Morin M, Popovic R (2010) Effect of core-shell copper oxide nanoparticles on cell culture morphology and photosynthesis (photosystem II energy distribution) in the green alga, Chlamydomonas reinhardtii. Aquat Toxicol 96:109–114
Sendra M, Moreno-Garrido I, Blasco J, Araújo CVM (2018) Effect of erythromycin and modulating effect of CeO2 NPs on the toxicity exerted by the antibiotic on the microalgae Chlamydomonas reinhardtii and Phaeodactylum tricornutum. Environ Pollut 242:357–366
Wang Z, Li J, Zhao J, Xing B (2011) Toxicity and internalization of CuO nanoparticles to prokaryotic alga Microcystis aeruginosa as affected by dissolved organic matter. Environ Sci Technol 45:6032–6040
Zilliges Y, Kehr JC, Meissner S, Ishida K, Mikkat S, Hagemann M, Kaplan A, Borner T, Dittmann E (2011) The cyanobacterial hepatotoxin microcystin binds to proteins and increases the fitness of microcystis under oxidative stress conditions. PLoS ONE 6:e17615
This study was supported by Natural Science Foundation of Jiangsu Province (No. BK20161404) and National Natural Science Foundation of China (Grant No. 41571130061).
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Guiqi Zhao and Di Wu have contributed equally and should be considered co-first authors.
About this article
Cite this article
Zhao, G., Wu, D., Cao, S. et al. Effects of CeO2 Nanoparticles on Microcystis aeruginosa Growth and Microcystin Production. Bull Environ Contam Toxicol 104, 834–839 (2020). https://doi.org/10.1007/s00128-020-02842-9
- CeO2 nanoparticles
- Microcystis aeruginosa