Skip to main content

Advertisement

Springer Nature Link
Log in

Lysosomal Membrane Stability in Hemocytes and Micronuclei in Gills of Perumytilus purpuratus Lamarck 1819 (Bivalvia: Mytilidae) Exposed to Copper

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

The aim of this study was to determine the cytotoxic and genotoxic effects of copper on the bivalve Perumytilus purpuratus. The individuals were exposed to three copper concentrations: 1, 30 and 45 μg L−1 for 24, 48 and 96 h. Lysosomal membrane stability in hemocytes was determined through the neutral red retention time (NRRT) and micronucleus (MN) frequency tests in hemocytes and gills. The results show that the NRRT decreased significantly at 30 μg L−1 after 48 h of exposure. The frequency of MN was significantly greater in gills after 24 h in all concentrations tested. Copper is cytotoxic from 30 μg L−1 and genotoxic from 1 μg L−1. The use of these biomarkers of effects in P. purpuratus is proposed as an early warning tool for monitoring in environmental assessment of coastal ecosystems impacted by mining activities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Baršiené J, Šyvokiené J, Bjornstad A (2006) Induction of micronuclei and other nuclear abnormalities in mussels exposed to bisphenol A, diallyl phthalate and tetrabromodiphenyl ether-47. Aquat Toxicol 78:105–108

    Article  Google Scholar 

  • Baršienė J, Andreikéitė L, Garnaga G, Rybakovas A (2008) Genotoxic and cytotoxic effects in the bivalve mollusks Macoma balthica and Mytilus edulis from the Baltic Sea. Ekologija 54:44–50

    Article  Google Scholar 

  • Bolognesi C, Hayashi M (2011) Micronucleus assay in aquatic animals. Mutagenesis 26:205–213

    Article  CAS  Google Scholar 

  • Bolognesi C, Landini E, Roggieri P, Fabbri R, Viarengo A (1999) Genotoxicity Biomarkers in the Assessment of Heavy Metal Effects in Mussels: experimental Studies. Environ Mol Mutagen 33:287–292

    Article  CAS  Google Scholar 

  • Bolognesi C, Frenzilli G, Lasagna C, Perrone E, Roggieri P (2004) Genotoxicity biomarkers in Mytilus galloprovincialis: wild versus caged mussels. Mutat Res 552:153–162

    Article  CAS  Google Scholar 

  • Bosse C, Rosen G, Colvin M, Earley P, Santore R, Rivera-Duarte I (2014) Copper bioavailability and toxicity to Mytilus galloprovincialis in Shelter Island Yacht Basin, San Diego. CA. Mar. Pollut. Bull. 85:225–234

    Article  CAS  Google Scholar 

  • Brown RJ, Galloway TS, Lowe D, Browne MA, Dissanayake A, Jones MB, Depledge MH (2004) Differential sensitivity of three marine invertebrates to cooper assessed using multiple biomarkers. Aquat Toxicol 66:267–278

    Article  CAS  Google Scholar 

  • Campillo J, Martínez-Gómez C, Fernández B, Benedicto J, Valdés N (2006) Biological effects of contaminats in mussels (Mytilus galloprovincialis) from the Iberian Mediterranean coast. In: Proceedings of the workshop on the med pol biological effects program: achievements and future orientations, pp 101–113

  • Castilla JC (1981) Perspectivas de investigación en estructura y dinámica de comunidades intermareales rocosas de Chile Central. II. Depredadores de alto nivel trófico. Medio Ambiente 5:190–215

    Google Scholar 

  • Castilla J (1983) Environmental impact in sandy beaches of copper mine tailings at Chañaral, Chile. Mar Pollut Bull 14:459–464

    Article  CAS  Google Scholar 

  • Fernández B, Campillo JA, Mart|nez-Gomez C, Benedicto J (2011) Micronuclei and other nuclear abnormalities in mussels (Mytilus galloprovi ncialis) as biomarkers of cyto-genotoxic pollution in mediterranean waters. Environ Mol Mutagen 52:479–491

    Article  Google Scholar 

  • Harding J, Couturier C, Parsons G, Ross NW (2004) Evaluation of the neutral red assay as a stress response indicator in cultivated mussels (Mytilus spp.) in relation to post-harvest processing activities and storage conditions aquaculture. Aquaculture 231:315–316

    Article  Google Scholar 

  • Izquierdo JI, Machado G, Ayllon F, d’Amico V, Bala LO, Vallarino E, Elias R, Garcia-Vazquez E (2003) Assessing pollution in coastal ecosystem: a preliminary survey using the micronucleus test in the mussel Mytilus edulis. Ecotoxicol Environ Saf 55:24–29

    Article  CAS  Google Scholar 

  • Jara C, Gaete H, Lobos G, Hidalgo M (2014) Oxidative stress in the mollusk Echinolittorina peruviana (Gasteropoda: Littorinidae, Lamarck, 1822) and trace metals in coastal sectors with mining activity. Ecotoxicology 23(6):1099–1108. https://doi.org/10.1007/s10646-014-1253-3

    Article  CAS  Google Scholar 

  • Jha AN, Dogra V, Turner A, Millward GE (2005) Impact of low doses of tritium on the marine mussel, Mytilus edulis: genotoxic effects and tissue-specific bioconcentration. Mutat Res 586:47–57

    Article  CAS  Google Scholar 

  • Kalpaxis DL, Theos C, Xaplanteri MA, Dinos GP, Catsiki AV, Leotsinidis M (2004) Application of a biomarker suite including evaluation of translation efficiency in Mytilus galloprovincialis cells. Environ Res 94:211–220

    Article  CAS  Google Scholar 

  • Klobučar GI, Pavlica V, Erben M, Papeš D (2003) Application of the micronucleus and comet assays to mussel Dreissena polymorpha haemocytes for genotoxicity monitoring of freshwater environments. Aquat Toxicol 64:15–23

    Article  Google Scholar 

  • Koukozika N, Dimitriadis VK (2005) Multiple biomarker comparison in Mytilus galloprovincialis from the Greece coast: “lysosomal membrane stability, neutral red retention, micronucleus”. Ecotoxicology 14:449–463

    Article  Google Scholar 

  • Machado-Schiaffino O, Bala GL, Garcia-Vazquez E (2009) Recovery of normal cytogenetic records in mussels after cessation of pollutant effluents in Puerto Madryn (Patagonia, Argentina). Estuaries Coasts 32:813–818

    Article  Google Scholar 

  • Martínez-Gómez C, Benedicto JJ, Campillo JA, Moore M (2008) Application and evaluation of the neutral red retention (NRR) assay for lysosomal stability in mussel populations along the Iberian Mediterranean coast. J Environ Monit 10:490–499

    Article  Google Scholar 

  • Medina M, Andrade S, Faugeron S, Lagos N, Mella D, Correa JA (2005) Biodiversity of rocky intertidal benthic communities associated with copper mine tailing discharges in northern Chile. Mar Pollut Bull 50:396–409

    Article  CAS  Google Scholar 

  • Nicholson S (2003) Lysosomal membrane stability, phagocytosis and tolerance to emersión in the mussel Pernal viridis (Bivalvia: mytilidae) following exposure to acute, sublethal, copper. Chemosphere 52:1147–1151

    Article  CAS  Google Scholar 

  • Parra S, Bravo MA, Quiroz W, Querol X, Paipa C (2015) Distribution and pollution assessment of trace elements in marine sediments in the Quintero Bay (Chile). Mar Pollut Bull 99:256–263

    Article  CAS  Google Scholar 

  • Pavlica M, Podrug M, Štambuk A, Cvjetko P, Klobučar GI (2008) Seasonal variability in micronuclei induction in haemocytes of mussels along the eastern Adriatic coast. Pol J Environ Stud 17:765–771

    Google Scholar 

  • Riveros A, Zúñiga M, Hernandez A, Camaño A (2002) Cellular biomarkers in native and transplanted populations of the mussel Perumytilus purpuratus in the intertidal zones of san Jorge bay, Antofagasta Chile. Arch Environ Contam Toxicol 42:303–312

    Article  CAS  Google Scholar 

  • Riveros A, Zuñiga M, Larrain A (2003) Copper Metallothionein-like Proteins as Exposure Biomarker in Native and Transplanted Intertidal Populations of the Mussel Perumytilus purpuratus from San Jorge Bay, Antofagasta, Chile. Bull Environ Contam Toxicol 70:233–241

    Article  CAS  Google Scholar 

  • Sadikaj R, Panariti E, Arapi D (2010) Monitoring of toxic residues in bivalve molluscs along the Adriatic coastal line of Albania. Nat Monten Podgor 9(3):321–329

    Google Scholar 

  • Salamanca MA, Jara B, Rodríguez T (2004) Cu, Pb and Zn levels in Perumytilus purpuratus and water san Jorge bay, northern of Chile. Gayana 68(1):53–62

    Google Scholar 

  • Scarpato R, Migliore L, Alfinito-Cognetti G, Barale R (1990) Induction of micronuclei in gill tissue of Mytilus galloprovincialis exposed to polluted marine waters. Mar Pollut Bull 21:74–80

    Article  CAS  Google Scholar 

  • Thiagaran R, Gopalakrishnan S, Thilagan H (2006) Inmunomodulation in the marine green mussels Perna viridis exposed exposed to Sub-Letal concentrations of Cu and Hg. Arch Environ Contam Toxicol 51:392–399

    Article  Google Scholar 

  • Valdés J, Román D, Guiñez M, Rivera L, Ávila J, Cortés JP, Castillo A (2015) Trace metal variability in coastal waters of San Jorge Bay, Antofagasta, Chile: an environmental evaluation and statistical approach to propose local background levels. Mar. Pollut. Bull. https://doi.org/10.1016/j.marpolbul.2015.08.035

    Article  Google Scholar 

  • Vianna VI, Marques de Oliveira I, Coelho JS, Ferraz JD, Pêgas JH, da Silva J (2007) Assessment of Environmental stress by the micronucleus and comet assays on Limnoperna fortunei exposed to Guaíba hydrographic region samples (Brazil) under laboratory conditions. Mutat Res 628:76–86

    Article  Google Scholar 

  • Vincent-Hubert F, Arini A, Gourlay-Francé C (2011) Early genotoxic effects in gill cells and haemocytes of Dreissena polymorpha exposed to cadmium, B[α]P and a combination of B[α]P and Cd. Mutat Res 723:26–35

    Article  CAS  Google Scholar 

  • Wrisberg MN, Bilbo CM, Spliid H (1992) Induction of micronuclei in hemocytes of Mytilus edulis and statistical analysis. Ecotoxicol Environ Saf 23:191–205

    Article  CAS  Google Scholar 

  • Zhang Z, Li X (2006) Evaluation of the effects of grading and starvation on the lisosomal membrane stability in pacific oyster, Crassostrea gigas (Tunberg) by using neutral red retention assay. Aquaculture 256:537–541

    Article  CAS  Google Scholar 

  • Zuykov M, Pelletier E, Harper DT (2013) Bivalve mollusks in metal pollution studies: from bioaccumulation to biomonitoring. Chemosphere 93(2):201–208

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The study was funded by Direction of Research DIUV, Universidad de Valparaíso, Chile.

Author information

Authors and Affiliations

  1. Escuela de Ingeniería en Medioambiente, Facultad de Ingeniería, Universidad de Valparaíso, Av. Brasil 2140, Valparaíso, Chile

    Hernán Gaete, Patricio Espinoza & Daniel Fernández

  2. Centro de Investigación y Gestión de Recursos Naturales CIGREN, Universidad de Valparaíso, Av. Gran Bretaña 1111, Playa Ancha, Valparaiso, Chile

    Hernán Gaete

  3. Instituto de Biología, Facultad de Ciencias, Universidad de Valparaíso, Av. Gran Bretaña 1111, Playa Ancha, Valparaiso, Chile

    Rosa Guerra

Authors
  1. Hernán Gaete
    View author publications

    Search author on:PubMed Google Scholar

  2. Rosa Guerra
    View author publications

    Search author on:PubMed Google Scholar

  3. Patricio Espinoza
    View author publications

    Search author on:PubMed Google Scholar

  4. Daniel Fernández
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Hernán Gaete.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gaete, H., Guerra, R., Espinoza, P. et al. Lysosomal Membrane Stability in Hemocytes and Micronuclei in Gills of Perumytilus purpuratus Lamarck 1819 (Bivalvia: Mytilidae) Exposed to Copper. Bull Environ Contam Toxicol 103, 796–801 (2019). https://doi.org/10.1007/s00128-019-02737-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-019-02737-4

Keywords