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Are Oxidative Stress Biomarkers Sensitive to Environmental Concentrations of Chlorpyrifos Exposed to the Freshwater Crab, Zilchiopsis collastinensis (Decapoda; Trichodactylidae)?

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Abstract

Global trends in pesticide use can increase aquatic pollution and affect resident fisheries. Crabs exposed to organophosphate pesticides, such as chlorpyrifos, may increase production of reactive oxygen species (ROS), affecting the pro-oxidant/antioxidant balance. Zichiopsis collastinensis crabs were exposed to environmentally relevant concentrations of chlorpyrifos (0.1 and 0.5 µg L−1). Effects on the oxidative stress enzymes catalase, superoxide dismutase, glutathione S-transferases, glutathione reductase, and on thiobarbituric acid reactive substances and hydrogen peroxide concentrations were evaluated at four intervals during 96 h exposures. Exposures caused decreased GST activity and increased H2O2 levels in gills. There were modifications of GST, CAT and SOD activities in the hepatopancreas after 12 h of exposure, and an increase of H2O2 levels at every exposure interval observed. The present study proved that chlorpyrifos lead to oxidative stress in Z. collastinensis. However other enzymatic/non-enzymatic responses should be further investigated in order to be included as part of a battery of biomarkers, together with H2O2 levels, which is a parameter highly recommended to be taken into account.

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Acknowledgements

This study was supported by the Grants PICT 2015 2811 and PICT 2013 1348 (FONCYT); (CAI + D 50020150100035LI (UNL) and EXA 795/16 (UNDMP).

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Authors and Affiliations

  1. INALI (CONICET-UNL), Ciudad Universitaria, 3000, Santa Fe, Argentina

    C. L. Negro & P. Collins

  2. ESS (FBCB-UNL), Ciudad Universitaria, 3000, Santa Fe, Argentina

    C. L. Negro & P. Collins

  3. IIMyC (UNMdP-CONICET), Funes 3350, 7600, Mar del Plata, Argentina

    F. G. Iturburu & M. L. Menone

  4. IIB (UNMdP-CONICET), Funes 3250, 7600, Mar del Plata, Argentina

    J. Mendieta

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  1. C. L. Negro
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  2. F. G. Iturburu
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  3. J. Mendieta
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  4. M. L. Menone
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  5. P. Collins
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Correspondence to C. L. Negro.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Negro, C.L., Iturburu, F.G., Mendieta, J. et al. Are Oxidative Stress Biomarkers Sensitive to Environmental Concentrations of Chlorpyrifos Exposed to the Freshwater Crab, Zilchiopsis collastinensis (Decapoda; Trichodactylidae)?. Bull Environ Contam Toxicol 103, 405–410 (2019). https://doi.org/10.1007/s00128-019-02643-9

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  • DOI: https://doi.org/10.1007/s00128-019-02643-9

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