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Development and Validation of an Analytical Method for the Detection and Quantification of Bromazepam, Clonazepam and Diazepam by UPLC-MS/MS in Surface Water

Abstract

The development of analytical methods capable of determining micropollutants is essential for quality control of drinking water. Benzodiazepines, a class of pharmaceuticals with anxiolytic properties, have received increasing attention as micropollutants. The purpose of this study was to develop an analytical method for determination of three benzodiazepine drugs (bromazepam, clonazepam and diazepam) in surface water. For the extraction of the matrix analytes, SPE cartridges (C18, 500 mg/3 mL) were used. The method was validated according to the quality criteria of the USEPA 8000D Validation Guide. The developed and validated method showed recovery values between 57 and 100%, RSD < 20% and R2 > 0.9949. LD ranged between 2.70 and 5.00 ng L−1 for bromazepam and clonazepam respectively whereas LQ was 0.01 μg L−1 for all analytes. The matrix affected the signal intensity of clonazepam thus evidencing the matrix effect by analysis statistic (F test).

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Acknowledgements

The authors acknowledge the financial support provided by the Coordination and Improvement of Higher Level or Education Personnel (CAPES) to the first author (Process 1589040/2016); by the National Council for Scientific and Technological Development (CNPq) (Process 308.335/2017-1) to the third author and; by the Carlos Chagas Filho Research Support Foundation (FAPERJ) (Processes E-26/202.894/2018 and E-26/202.793/2015) to the third and fourth authors, respectively.

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Correspondence to Frederico Goytacazes de Araujo.

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de Araujo, F.G., Bauerfeldt, G.F., Marques, M. et al. Development and Validation of an Analytical Method for the Detection and Quantification of Bromazepam, Clonazepam and Diazepam by UPLC-MS/MS in Surface Water. Bull Environ Contam Toxicol 103, 362–366 (2019). https://doi.org/10.1007/s00128-019-02631-z

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Keywords

  • PPCPs
  • Micropollutants
  • Surface waters
  • Benzodiazepines
  • UPLC-MS/MS