Occurrence of Acetamiprid Residues in Water Reservoirs in the Cotton Basin of Northern Benin


An Ultra performance liquid chromatography (UPLC) coupled to UV detection method was developed to determine acetamiprid residues in water reservoirs of northern Benin, close to cotton fields. The quantification limit of this method was 0.2 µg L−1 acetamiprid in water, its precision ranged between 8% and 22%, and its trueness between 99% and 117% (for concentrations ranging from 0.2 to 5.0 µg L−1). Acetamiprid residues were determined in water samples collected in four reservoirs from northern Benin during the phytosanitary treatment period of cotton. The minimum and maximum concentrations of acetamiprid residues in water were 0.2 and 7.7 µg L−1, respectively. These levels do not represent any risk for human consumption of this water, but indicate a regular use of acetamiprid, possibly together with other pesticides which could be more harmful for both humans and aquatic species.

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  1. Agbohessi PT, Imorou- Toko I, Ouédraogo A, Jauniaux T, Kestemont P (2015) Assessment of the health status of wild fish inhabiting a cotton basin heavily impacted by pesticides in Benin (West Africa). Sci Total Environ 506–507:567–584. https://doi.org/10.1016/j.scitotenv.2014.11.047

    Article  CAS  Google Scholar 

  2. ASECNA (2016) (Agence pour la sécurité de la navigation aérienne en Afrique et à Madagascar), Station de Kandi, Département de l’Alibori. Données mensuelles sur la température, la pluviométrie et l’humidité Kandi, Département de l’Alibori. Données mensuelles sur la température, la pluviométrie et l’humidité relative de 2003 à 2012

  3. EC (European Commission) (2004) SANCO/1392/2001-Review Report for the Active Substance Acetamiprid. Protection directorate-general. E1-Plant health. P1-33

  4. EC (European Council) (1998) Council Directive 98/83/EC of 3 Nov 1998 on the quality of water intended for human consumption. Official Journal L 330:32–54

    Google Scholar 

  5. EFSA (European Food Safety Authority) (2016) Conclusion on the peer review of the pesticide risk assessment of the active substance acetamiprid. EFSA J 14:4610–4636. https://doi.org/10.2903/j.efsa.2016.4610

    CAS  Article  Google Scholar 

  6. EPA (United States Environmental protection Agency) (2002) Pesticide fact sheet: acetamiprid. Available at https://www3.epa.gov/pesticides/chem_search/reg_actions/registration/fs_PC-099050_15-Mar-02.pdf

  7. Gouda AI, Mehoba MHL, Toko II, Scippo ML, Kestemont P, Schiffers B (2018) Comparison of drift of two types of sprayers used in cotton production in Benin. Biotechnol Agron Soc Environ 22:1–12. https://popups.uliege.be/1780-4507/index.php?id=16431

  8. Guedegba NL, Imorou Toko I, Agbohessi PT, Oreins N, François L, Palluel O, Mandiki SNM, Porcher JM, Schiffers B, Scippo ML, Kestemont P. A multi-biomarker assessment of chronic effects of an insecticide Acer 35 EC on Nile Tilapia Oreochromis niloticus under laboratory conditions. Eurotox 2018 conference

  9. Han W, Tian Y, Shen X (2018) Human exposure to neonicotinoid insecticides and the evaluation of their potential toxicity: an overview. Chemosphere 192:59–65. https://doi.org/10.1016/j.chemosphere.2017.10.149

    Article  CAS  Google Scholar 

  10. Hladik ML, Kolpin DW, Kuivila KM (2014) Widespread occurrence of neonicotinoid insecticides in streams in a high corn and soybean producing region, USA. Environ Pollut 193:189–196. https://doi.org/10.1016/j.envpol.2014.06.033

    Article  CAS  Google Scholar 

  11. Hoyle S, Code A (2016) Neonicotinoids in California’s surface waters: a preliminary review of potential risk to aquatic invertebrates. The Xerces Society for Invertebrate Conservation, pp 1–17

  12. Imamura T, Yanagawa Y, Nishikawa K, Matsumoto N, Sakamoto T (2010) Two cases of acute poisoning with acetamiprid in humans. Clin Toxicol 48:851–853. https://doi.org/10.3109/15563650.2010.517207

    Article  Google Scholar 

  13. Jabeen F, Chaudhry AS, Manzoor S, Shaheen T (2015) Examining pyrethroids, carbamates and neonicotinoids in fish, water and sediments from the Indus River for potential health risks. Environ Monit Assess 187:1–11. https://doi.org/10.1007/s10661-015-4273-4

    Article  CAS  Google Scholar 

  14. Kim BM, Park JS, Choi JH, Abd El-Aty AM, Na TW, Shim JH (2012) Residual determination of clothianidin and its metabolites in three minor crops via tandem mass spectrometry. Food Chem 131:1546–1551. https://doi.org/10.1016/j.foodchem.2011.09.134

    Article  CAS  Google Scholar 

  15. MAEP (Ministère Béninois de l’Agriculture, de l’Elevage et de la Pêche) (2015) Liste des produits phytosanitaires disponibles pour la campagne agricole dans les départements du Borgou-Alibori. Fiche technique Cotonou (Bénin)

  16. Morrissey CA, Mineau P, Devries JH, Sanchez-Bayo F, Liess M, Cavallaro MC, Liber K (2015) Neonicotinoid contamination of global surface waters and associated risk to aquatic invertebrates: A review. Environ Int 74:291–303. https://doi.org/10.1016/j.envint.2014.10.024

    Article  CAS  Google Scholar 

  17. Park JY, Choi JH, Kim BM, Park JH, Cho SK, Ghafar MW, Abd El-Aty AM, Shim JH (2010) Determination of acetamiprid residues in zucchini grown under greenhouse conditions: application to behavioral dynamics. Biomed Chromatogr 25:136–146. https://doi.org/10.1002/bmc.1529

    Article  CAS  Google Scholar 

  18. Phua DH, Lin CC, Wu ML, Deng JF, Yang CC (2009) Neonicotinoid insecticides: an emerging cause of acute pesticide poisoning. Clin Toxicol 47:336–341. https://doi.org/10.1080/15563650802644533

    Article  CAS  Google Scholar 

  19. Raj JS, Baby J (2015) Impact of acetamiprid toxicity on biochemical biomarkers (protein and carbohydrate) in some tissues of the fish Oreochromis mossambicus. Int J Zool Res 11:222–227. https://doi.org/10.3923/ijzr.2015.222.227

    Article  CAS  Google Scholar 

  20. Sanchez-Bayo F, Hyne RV (2014) Detection and analysis of neonicotinoids in river waters – development of a passive sampler for three commonly used insecticides. Chemosphere 99:143–151. https://doi.org/10.1016/j.chemosphere.2013.10.051

    Article  CAS  Google Scholar 

  21. SANTE/11945/2015 (2015) Analytical quality control and method validation procedures for pesticide residues analysis in food and feed

  22. Seccia S, Fidente P, Barbini DA, Morrica P (2005) Multiresidue determination of nicotinoid insecticide residues in drinking water by liquid chromatography with electrospray ionization mass spectrometry. Anal Chim Acta 553:21–26. https://doi.org/10.1016/j.aca.2005.08.006

    Article  CAS  Google Scholar 

  23. Todani M, Kaneko T, Hayashida H, Kaneda K, Tsuruta R, Kasaoka S, Maekawa T (2008) Acute poisoning with neonicotinoid insecticide acetamiprid. Chudoku Kenkyu 21:387–390

    Google Scholar 

  24. Wang P, Yang X, Wang J, Cui J, Dong AJ, Zhao HT, Zhang LW, Wang ZY, Xu RB, Li WJ, Zhang YC, Zhang H, Jing J (2012) Multi-residue method for determination of seven neonicotinoid insecticides in grains using dispersive solid-phase extraction and dispersive liquid–liquid micro-extraction by high performance liquid chromatography. Food Chem 134:1691–1698. https://doi.org/10.1016/j.foodchem.2012.03.103

    Article  CAS  Google Scholar 

  25. Xiao Z, Li X, Wang X, Shen J, Ding S (2011) Determination of neonicotinoid insecticides residues in bovine tissues by pressurized solvent extraction and liquid chromatography–tandem mass spectrometry. J Chromatogr B 879:117–122. https://doi.org/10.1016/j.jchromb.2010.11.008

    Article  CAS  Google Scholar 

  26. Zhiming X, Yunxia Y (2013) Determination of neonicotinoid insecticides residues in eels using subcritical water extraction and ultra-performance liquid chromatography–tandem mass spectrometry. Anal Chim Acta 777:32–40. https://doi.org/10.1016/j.aca.2013.03.026

    Article  CAS  Google Scholar 

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This study was performed with the financial support of the ARES-CCD (Academy of Research and Higher Education-Committee for Development Cooperation, Belgium) (Project AquaTox-Benin).

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Correspondence to Marie-Louise Scippo.

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Zoumenou, B.G.Y.M., Aïna, M.P., Imorou Toko, I. et al. Occurrence of Acetamiprid Residues in Water Reservoirs in the Cotton Basin of Northern Benin. Bull Environ Contam Toxicol 102, 7–12 (2019). https://doi.org/10.1007/s00128-018-2476-4

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  • Acetamiprid
  • Surface water
  • Agriculture
  • Cotton
  • Benin