Skip to main content

Advertisement

SpringerLink
Log in

The Chronic Exposure to Discharges of Sabal Drain Induces Oxidative Stress and Histopathological Alterations in Oreochromis niloticus

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

To study whether the effluents of Sabal drain could affect the health status of Oreochromis niloticus; site2 (south part of main canal), site3 (at the canal outlet), site4 (north part of main canal) and site1 (reference site) were selected. Compared to the reference fish, activities of superoxide dismutase and glutathione peroxidase in addition to glutathione reduced and thiobarbituric acid reactive substances concentrations of the gills showed significant (p < 0.05) increase with different levels in all studied sites. Whereas, catalase activities revealed significant (p < 0.05) decrease in all studied sites with maximum decrease in site3. The recorded histopathological lesions were correlated with the distance from discharge point. Gills, liver and kidney of site3 had the worst histopathological conditions based on the frequency of alterations appearance and size of the affected areas. While, the regressive alterations that recorded in site2 and site4 suggest the onset of adaptive histological mechanisms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abdel-Khalek AA (2015) Risk assessment, bioaccumulation of metals and histopathological alterations in Nile tilapia (Oreochromis niloticus) facing degraded aquatic conditions. Bull Environ Contam Toxicol 94:77–83

    Article  CAS  Google Scholar 

  • Abdel-Khalek AA, Elhaddad E, Mamdouh S, Marie M-A (2016) Assessment of metal pollution around Sabal drainage in river Nile and its impacts on bioaccumulation level, metals correlation and human risk hazard using Oreochromis niloticus as a bioindicator. Turk J Fish Aquat Sci 16:227–239

    Google Scholar 

  • Aebi H (1984) Catalase in vitro. Method Enzymol 105:121–126

    Article  CAS  Google Scholar 

  • Al-Bairuty GA, Shawa BJ, Handy RD, Henry TB (2013) Histopathological effects of waterborne copper nanoparticles and copper sulphate on the organs of rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 126:104–115

    Article  CAS  Google Scholar 

  • Atli G, Alptekin O, Tukel S, Canli M (2006) Response of catalase activity to Ag2+, Cd2+, Cr2+, Cu2+ and Zn2+ in five tissues of freshwater fish Oreochromis niloticus. Comp Biochem Physiol C 143:218–224

    Article  CAS  Google Scholar 

  • Authman MMN, Abbas HH, Abbas WT (2013) Assessment of metal status in drainage canal water and their bioaccumulation in Oreochromis niloticus fish in relation to human health. Environ Monit Assess 185:891–907

    Article  CAS  Google Scholar 

  • Barim O, Karatepe M (2010) The effects of pollution on the vitamins A, E, C, beta-carotene contents and oxidative stress of the freshwater crayfish, Astacus leptodactylus. Ecotoxicol Environ Saf 73:138–142

    Article  CAS  Google Scholar 

  • Behera M, Dandapat J, Rath CC (2014) Effect of heavy metals on growth response and antioxidant defense protection in Bacillus cereus. J Basic Microbiol 54:1201–1209

    Article  CAS  Google Scholar 

  • Bernet D, Schmidt H, Meier W, Burkhardt-Holm P, Wahli T (1999) Histopathology in fish: proposal for a protocol to assess aquatic pollution. J Fish Dis 22:25–34

    Article  Google Scholar 

  • Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888

    CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Burton CA, Hatlelid K, Divine K, Carter DE, Fernando Q, Brendel K, Gandolfi AJ (1995) Glutathione effects on toxicity and uptake of mercuric chloride and sodium arsenite in rabbit renal cortical slices. Environ Health Perspect 103:81–84

    Article  CAS  Google Scholar 

  • Camargo MMP, Martinez CBR (2007) Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotrop Ichthyol 5(3):327–336

    Article  Google Scholar 

  • Cao L, Huang W, Shan X, Ye Z, Dou S (2012) Tissue-specific accumulation of cadmium and its effects on antioxidative responses in Japanese flounder juveniles. Environ Toxicol Pharmacol 33:16–25

    Article  CAS  Google Scholar 

  • Copaja SV, Pérez CA, VegaRetter C, Véliz D (2017) Heavy metal content in Chilean fish related to habitat use, tissue type and river of origin. Bull Environ Contam Toxicol 99:695–700

    Article  CAS  Google Scholar 

  • Coutinho C, Gokhale KS (2000) Selected oxidative enzymes and histopathological changes in the gills of Cyprinus carpio and Oreochromis mossambicus cultured in secondary sewage effluent. Water Res 34:2997–3004

    Article  CAS  Google Scholar 

  • Javed M, Ahmad I, Usmani N, Ahmad M (2016) Studies on biomarkers of oxidative stress and associated genotoxicity and histopathology in Channa punctatus from heavy metal polluted canal. Chemosphere 151:210–219

    Article  CAS  Google Scholar 

  • Jozefczak M, Remans T, Vangronsveld J, Cuypers A (2012) Glutathione is a key player in metal-induced oxidative stress defenses. Int J Mol Sci 13(12):3145–3175

    Article  CAS  Google Scholar 

  • Kosai P, Jiraungkoorskul W, Thammasunthorn T, Jiraungkoorskul K (2009) Reduction of copper-induced histopathological alterations by calcium exposure in Nile tilapia (Oreochromis niloticus). Toxicol Mech Method 19:461–467

    Article  CAS  Google Scholar 

  • Mani R, Meena B, Valivitanu K, Suresh A (2014) Glutathione-s-transeferase and catalase activity in different tissues of marine Catfish Arius arius on exposure to cadmium. Int J Pharm Pharm Sci 6(1):326–332

    Google Scholar 

  • McGovarin S, Sultana T, Metcalfe C (2018) Biological responses in brook trout (Salvelinus fontinalis) caged downstream from municipal wastewater treatment plants in the credit river, ON, Canada. Bull Environ Contam Toxicol 100:106–111

    Article  CAS  Google Scholar 

  • Monroy M, Maceda-Veiga A, de Sostoa A (2014) Metal concentration in water, sediment and four fish species from lake Titicaca reveals a large-scale environmental concern. Sci Total Environ 487:233–244

    Article  CAS  Google Scholar 

  • Nishikimi M, Appaji N, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 46(2):849–854

    Article  CAS  Google Scholar 

  • Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358

    Article  CAS  Google Scholar 

  • Pacheco M, Santos MA (2002) Biotransformation, genotoxic and histopathological effects of environmental contaminants in European eel (Anguilla anguilla L.). Ecotoxicol Environ Saf 53:331–347

    Article  CAS  Google Scholar 

  • Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    CAS  Google Scholar 

  • Ruas CBG, Carvalho CS, Araújo HSS, Espíndola ELG, Fernandes MN (2008) Oxidative stress biomarkers of exposure in the blood of cichlid species from a metal-contaminated river. Ecotoxicol Environ Saf 71:86–93

    Article  CAS  Google Scholar 

  • Saglam D, Atli G, Dogan Z, Baysoy E, Gurler C, Eroglu A, Canli M (2014) Response of the antioxidant system of freshwater fish (Oreochromis niloticus) exposed to metals (Cd, Cu) in differing hardness. Turk J Fish Aquat Sci 14:43–52

    Google Scholar 

  • Sampaio FG, Boijink CL, Oba ET, Santos LRB, Kalinin AL, Rantin FT (2008) Antioxidant defenses and biochemical changes in pacu (Piaractus mesopotamicus) in response to single and combined copper and hypoxia exposure. Comp Biochem Physiol C 147:43–51

    Google Scholar 

  • Singhal RN, Jain M (1997) Cadmium induced changes in the histology of kidneys of common carp, Cyprinus carpio (Cyprinidae). Bull Environ Contam Toxicol 58:456–461

    Article  CAS  Google Scholar 

  • Souid G, Souayed N, Yaktiti F, Maaroufi K (2014) Lead accumulation pattern and molecular biomarkers of oxidative stress in sea bream (Sparus aurata) under short-term metal treatment. Drug Chem Toxicol 15:1–8

    Google Scholar 

  • Tunçsoy M, Duran S, Ay Ö, Cicik B, Erdem C (2017) Effects of copper oxide nanoparticles on antioxidant enzyme activities and on tissue accumulation of Oreochromis niloticus. Bull Environ Contam Toxicol 99:360–364

    Article  CAS  Google Scholar 

  • Tyor AK, Pahwa K (2017) Pollutants induced oxidative stress, DNA damage and cellular deformities in Clarias gariepinus (burchell), from river Yamuna in Delhi region, India. Bull Environ Contam Toxicol 99:33–38

    Article  CAS  Google Scholar 

  • Xia J, Zhou HZ, Guang Hua LU (2013) Effects of selected metal oxide nanoparticles on multiple biomarkers in Carassius auratus. Biomed Environ Sci 26(9):742–749

    CAS  Google Scholar 

Download references

Acknowledgements

We thank the fisherman for his help. We especially appreciate the editor and the reviewers for their hard work.

Author information

Authors and Affiliations

  1. Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt

    Amr Adel Abdel-Khalek, Samar Mamdouh & Mohamed-Assem Saed Marie

  2. Department of Medical Science, Faculty of Dentistry, British University in Egypt, Cairo, Egypt

    Amr Adel Abdel-Khalek

  3. Pollution Department, National Institute of Oceanography and Fisheries, Alexandria, Egypt

    Engy Elhaddad

Authors
  1. Amr Adel Abdel-Khalek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Engy Elhaddad
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Samar Mamdouh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohamed-Assem Saed Marie
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Amr Adel Abdel-Khalek.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

This manuscript complies to the ethical rules applicable for this journal.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Abdel-Khalek, A.A., Elhaddad, E., Mamdouh, S. et al. The Chronic Exposure to Discharges of Sabal Drain Induces Oxidative Stress and Histopathological Alterations in Oreochromis niloticus. Bull Environ Contam Toxicol 101, 92–98 (2018). https://doi.org/10.1007/s00128-018-2366-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-018-2366-9

Keywords

Search

Navigation