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mRNA Analysis of Genes Encoded with Phytochelatin Synthase (PCS) in Rice Seedlings Exposed to Chromium: The Role of Phytochelatins in Cr Detoxification

Abstract

Transcriptional changes of genes encoded with phytochelatins synthase (PCS) was investigated in rice seedlings exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) using qRT-PCR. Our study demonstrates that both Cr variants initiated different responses of phytochelatin content and PCS activities in rice tissues. Six putative PCS genes were expressed differentially in response to both Cr species. Comparing gene expression between root/shoots, only LOC_Os05g34290.1 and LOC_Os06g01260.1 genes were expressed in similar patterns in Cr(VI) treatment, while none of them were expressed equally in Cr(III) treatments. Inconsistent expression of PCS genes in two Cr variants as well as in rice tissues were most likely related to its individual chemical properties and chemical speciation. Results presented here indicate that the role of phytochelatins in Cr detoxification between two Cr variants in rice was different and six putative PCS genes functioned differently in stimulating PCS activities and regulating phytochelatin formation.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No: 41761094) and The Guangxi Talent Highland for Hazardous Waste Disposal Industrialization.

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Correspondence to Xiao-Zhang Yu.

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Yu, XZ., Ling, QL., Li, YH. et al. mRNA Analysis of Genes Encoded with Phytochelatin Synthase (PCS) in Rice Seedlings Exposed to Chromium: The Role of Phytochelatins in Cr Detoxification. Bull Environ Contam Toxicol 101, 257–261 (2018). https://doi.org/10.1007/s00128-018-2362-0

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Keywords

  • Chromium
  • Genes
  • Phytochelatins synthase
  • Rice