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Effect of Nitrogen Compounds on Shrimp Litopenaeus vannamei: Histological Alterations of the Antennal Gland

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Abstract

Two experimental modules with different stocking densities (M1 = 70 and M2 = 120 shrimp m−2) were examined weekly during 72-day culture cycle at low-salinity water (1.9 g L−1) and zero-water exchange to examine the effects of water quality deterioration on the antennal gland (AG) of shrimp. Results showed survival rates of 87.7% and 11.9% in M1 and M2, respectively. Water temperature, pH, dissolved oxygen, and chlorophyll a were not significantly different between modules but the concentrations of the nitrogen compounds were significantly different between modules with the exception of nitrite-N, showing a higher histological alteration index in M2 (32 ± 10) than M1 (22 ± 0) with a strong correlation with the nitrogen compounds. During the last weeks was evidenced in M1 inflammation and hemocytic and hemolymph infiltration, while in M2, melanization, hemocytic melanized nodules and cells with kariorrexis.

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Acknowledgements

Study supported by the Program UNAM-DGAPA-PAPIIT IN201916 “Evaluación de efectos letales y subletales de amonio, nitritos y nitratos en camarón Litopenaeus vannamei en aguas de baja salinidad”. Also was supported by the Red PRODEP “Contaminación acuática: niveles y efectos” (año 3), and by the research grant from CONACYT through the CONACYT INFR-2013 project 204818, 2017710219-Morales-Covarrubias MS and Fundación Produce Colima 06-2011-0018. Authors thank H Bojórquez-Leyva and A Velázquez-Garay for their assistance.

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Authors and Affiliations

  1. Posgrado en Ciencias del Mar y Limnología, Unidad Académica Mazatlán, Universidad Nacional Autónoma de México, 82040, Mazatlán, Sinaloa, Mexico

    Marcela G. Fregoso-López

  2. Centro de Investigación en Alimentación y Desarrollo, A.C., 82000, Mazatlán, Sinaloa, Mexico

    María S. Morales-Covarrubias

  3. Instituto Tecnológico de Mazatlán, 82070, Mazatlán, Sinaloa, Mexico

    Miguel A. Franco-Nava

  4. Lab. Bioingeniería Costera. Escuela Nacional de Ingeniería Pesquera y Centro Multidisciplinario de Bahía de Banderas, Universidad Autónoma de Nayarit, Nayarit, Mexico

    Jesús T. Ponce-Palafox

  5. Posgrado en Ciencias Agropecuarias, Colegio de Ciencias Agropecuarias, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, Mexico

    Juan F. Fierro-Sañudo

  6. Programa de Posgrado en Recursos Acuáticos, Facultad de Ciencias del Mar, Universidad Autónoma de Sinaloa, Mazatlán, Sinaloa, Mexico

    Javier Ramírez-Rochín

  7. Instituto de Ciencias del Mar y Limnología, Unidad Académica Mazatlán, Universidad Nacional Autónoma de México, Mazatlán, Sinaloa, Mexico

    Federico Páez-Osuna

Authors
  1. Marcela G. Fregoso-López
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  2. María S. Morales-Covarrubias
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  3. Miguel A. Franco-Nava
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  4. Jesús T. Ponce-Palafox
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  5. Juan F. Fierro-Sañudo
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  6. Javier Ramírez-Rochín
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  7. Federico Páez-Osuna
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Corresponding author

Correspondence to Federico Páez-Osuna.

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Fregoso-López, M.G., Morales-Covarrubias, M.S., Franco-Nava, M.A. et al. Effect of Nitrogen Compounds on Shrimp Litopenaeus vannamei: Histological Alterations of the Antennal Gland. Bull Environ Contam Toxicol 100, 772–777 (2018). https://doi.org/10.1007/s00128-018-2349-x

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  • DOI: https://doi.org/10.1007/s00128-018-2349-x

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