Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity

  • Fernando G. Iturburu
  • María F. Simoniello
  • Sandra Medici
  • Ana M. Panzeri
  • Mirta L. Menone
Article

Abstract

Neonicotinoids are one of the most widely used insecticides in the world. DNA damage is considered an early biological effect which could lead to reproductive and carcinogenic effects. The present study aimed to evaluate DNA damage and bases oxidation as a mechanism of genotoxicity, on the freshwater fish Australoheros facetus acutely exposed to imidacloprid (IMI). The Comet assay with the nuclease ENDO III enzyme was performed for detecting pyrimidine bases oxidation using blood samples. Micronucleus and other nuclear abnormalities frequencies were also quantified. A significant increase of damage index at 100 and 1000 µg/L IMI was detected; while ENDO III score increased from 1 to 1000 µg/L IMI; varying both in a linear concentration-response manner. MN frequency increased in fish exposed to 1000 µg/L IMI. These results show that short-term exposures to environmentally relevant concentrations of IMI could affect the genetic integrity of fishes through oxidative damage.

Keywords

Neonicotinoid Fishes DNA fragmentation Micronucleus 

Notes

Acknowledgements

The authors acknowledge Dr. Andrew Collins (University of Oslo, Oslo Norway) for providing the enzyme Endonuclease III. The present study was supported by UNMDP (EXA 795/16) and FONCYT (PICT 2013-1348).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Fernando G. Iturburu
    • 1
  • María F. Simoniello
    • 2
  • Sandra Medici
    • 3
  • Ana M. Panzeri
    • 4
  • Mirta L. Menone
    • 1
  1. 1.Laboratorio de EcotoxicologíaInstituto de Investigaciones Marinas y Costeras (IIMyC)- UNMDP, CONICET, Facultad Cs. Ex. y Nat.Mar del PlataArgentina
  2. 2.Cátedra de Toxicología, Farmacología y Bioquímica Legal, Facultad de Bioquímica y Ciencias BiológicasUNLSanta FeArgentina
  3. 3.Fares Taie Instituto de AnálisisMar del PlataArgentina
  4. 4.Laboratorio de GenéticaInstituto de Investigaciones Marinas y Costeras (IIMyC)- UNMDP, CONICET, Facultad Cs. Ex. y Nat.Mar del PlataArgentina

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