Fifteen giant pandas (Ailuropoda melanoleuca) from the Chinese Conservation and Research Center for the Giant Panda (CCRCGP) in Bifengxia, Sichuan, China were analyzed for blood lead concentrations (Pb-B) during the 2017 breeding season. Thirteen of the 15 bears showed Pb-B below the method detection limit (MDL) of 3.3 µg/dL. The two remaining bears, although above the MDL, contained very low concentrations of lead of 3.9 and 4.5 µg/dL. All 15 giant pandas in this analysis had Pb-B concentrations that were within normal background concentrations for mammals in uncontaminated environments. For a threatened species, whose native country is plagued by reports of extremely high air pollution, our findings suggest that giant pandas at the CCRCGP are not absorbing lead at concentrations that would adversely affect their health.
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Barrett J, Livesey PJ (1985) Low level lead effects on activity under varying stress conditions in the developing rat. Pharmacol Biochem Behav 22(1):107–118
Bischoff K, Gaskill C, Erb HN, Ebel JG, Hillebrandt J (2010) Comparison of two methods for blood lead analysis in cattle: graphite-furnace atomic absorption spectrometry and Leadcare® II system. J Vet Diagn Invest 22(5):729–733
Chen TB, Zheng YM, Lei M, Huang ZC, Wu HT, Chen H, Fan KK, Yu K, Wu X, Tian QZ (2005) Assessment of heavy metal pollution in surface soils of urban parks in Beijing, China. Chemosphere 60(4):542–551
China National Bureau of Statistics (2010) http://population.city/china/. Accessed 1 August 2017
Cramer JC (2002) Population growth and local air pollution: methods, models, and results. Popul Dev Rev 28:22–52
Dierenfeld ES, Hintz HF, Robertson JB, Van Soest PJ, Oftedal OT (1982) Utilization of bamboo by the giant panda. J Nutr 112(4):636–641
Duan Q, Lee J, Liu Y, Chen H, Hu H (2016) Distribution of heavy metal pollution in surface soil samples in China: a graphical review. Bull Environ Contam Toxicol 97(3):303–309
Dudley B (2016) BP statistical review of world energy 2016
Helsel DR (2005) Nondetects and data analysis: statistics for censored environmental data. Wiley, New York
Huang Y, Li D, Zhou Y, Zhou Q, Li R, Wang C, Huang Z, Hull V, Zhang H (2012) Factors affecting the outcome of artificial insemination using cryopreserved spermatozoa in the giant panda (Ailuropoda melanoleuca). Zoo Biol 31(5):561–573
Ip CCM, Li XD, Zhang G, Wong CSC, Zhang WL (2005) Heavy metal and Pb isotopic compositions of aquatic organisms in the Pearl River Estuary, South China. Environ Pollut 138(3):494–504
Laughlin NK, Bowman RE, Levin ED, Bushnell PJ (1983) Neurobehavioral consequences of early exposure to lead in rhesus monkeys: effects on cognitive behaviors. In: Reproductive and developmental toxicity of metals. Springer, New York, pp 497–515
Ley RE, Hamady M, Lozupone C, Turnbaugh PJ, Ramey RR, Bircher JS, Schlegel ML, Tucker TA, Schrenzel MD, Knight R, Gordon JI (2008) Evolution of mammals and their gut microbes. Science 320(5883):1647–1651
Li D, Wintle NJP, Zhang G, Wang C, Luo B, Martin-Wintle MS, Owen MA, Swaisgood RR (2017) Analyzing the past to understand the future: natural mating yields better reproductive rates than artificial insemination in the giant panda. Biol Conserv 216:10–17
Liang F, Zhang G, Tan M, Yan C, Li X, Li Y, Li Y, Zhang Y, Shan Z (2010) Lead in children’s blood is mainly caused by coal-fired ash after phasing out of leaded gasoline in Shanghai. Environ Sci Technol 44(12):4760–4765
Liu J, Hull V, Yang W, Vina A, Chen X, Ouyang Z, Zhang H (eds) (2016) Pandas and people: coupling human and natural systems for sustainability. Oxford University Press, Oxford
Ma WC (2011) Lead in mammals. In: Beyer WN, Meador JP (eds) Environmental contaminants in biota: interpreting tissue concentrations, 2nd edn. CRC Press, Boca Raton, pp 595–604
Magellan D (2017) FDA warns against using Magellan Diagnostics LeadCare testing systems with blood obtained from a vein: FDA safety communication. http://www.leadcare2.com/getmedia/cd7f4554-c6c1-4561-9214-5aea58cc2fd5/80-0243-FDA-Notice.pdf.aspx. Accessed 4 October 2017
Magellan Diagnostics (2017) LeadCare II blood lead analyzer user’s guide. http://www.leadcare2.com/getmedia/73ac501b-35e3-4d74-b6a9-9e7fc51adef0/70-6551_Rev_07_User-s_Guide,_LeadCare_II_(PRINT)-1.pdf.aspx. Accessed 4 June 2017
Martin S, Griswold W (2009) Human health effects of heavy metals. Environ Sci Technol Brief Cit 15:1–6
McMichael AJ (2000) The urban environment and health in a world of increasing globalization: issues for developing countries. Bull World Health Organ 78(9):1117–1126
Mullan B, Marvin G (1999) Zoo culture. University of Illinois Press, Chicago
Neuman DR, Dollhopf DJ (1992) Lead levels in blood from cattle residing near a lead smelter. J Environ Qual 21(2):181–184
Ni W, Huang Y, Wang X, Zhang J, Wu K (2014) Associations of neonatal lead, cadmium, chromium and nickel co-exposure with DNA oxidative damage in an electronic waste recycling town. Sci Total Environ 472:354–362
Schaller GB (1985) Giant pandas of Wolong. University of Chicago Press, Chicago
Sobin C, Parisi N, Schaub T, de la Riva E (2011) A Bland–Altman comparison of the Lead Care® system and inductively coupled plasma mass spectrometry for detecting low-level lead in child whole blood samples. J Med Toxicol 7(1):24–32
Swaisgood R, Wang D, Wei F (2016) Ailuropoda melanoleuca. IUCN red list of system threatened species. http://www.iucnredlist.org/details/712/0. Accessed 30 October 2017
Swaisgood R, Wang D, Wei F (2017) Panda Downlisted but not Out of the Woods. Conserv Lett. https://doi.org/10.1111/conl.12355
US Fish and Wildlife Service (1998) Policy on giant panda permits. Fed Reg 63(166):45839–45854
Wang G, Su MY, Chen YH, Lin FF, Luo D, Gao SF (2006) Transfer characteristics of cadmium and lead from soil to the edible parts of six vegetable species in southeastern China. Environ Pollut 144(1):127–135
Wang Y, Chen P, Cui R, Si W, Zhang Y, Ji W (2010) Heavy metal concentrations in water, sediment, and tissues of two fish species (Triplohysa pappenheimi, Gobio hwanghensis) from the Lanzhou section of the Yellow River, China. Environ Monit Assess 165(1):97–102
Xie Z (2016) The 2016 international studbook for the giant panda (Ailuropoda melanoleuca). Chinese Association of Zoological Gardens, Beijing
Yan W, Mahmood Q, Peng D, Fu W, Chen T, Wang Y, Li S, Chen J, Liu D (2015) The spatial distribution pattern of heavy metals and risk assessment of moso bamboo forest soil around lead–zinc mine in Southeastern China. Soil Tillage Res 153:120–130
Zhang Y, Ruan L, Fasola M, Boncompagni E, Dong Y, Dai N, Gandini C, Orvini E, Ruiz X (2006) Little Egrets (Egretta garzetta) and trace-metal contamination in wetlands of China. Environ Monit Assess 118(1):355–368
We thank Magellan Diagnostics for providing information and for donating all testing supplies and equipment. We would like to thank the staff at the CCRCGP who collected all the samples for us. PDXWildlife, a 501(c)(3) non-profit, conducting research on giant pandas, provided interns to complete this study. As such, we owe a great deal of gratitude to Stephanie Crider who analyzed these samples. A special thank you goes to each of the reviewers for selflessly donating their time.
Conflict of interest
All authors have no conflict of interest to declare.
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Wintle, N.J.P., Martin-Wintle, M.S., Zhou, X. et al. Blood Lead Levels in Captive Giant Pandas. Bull Environ Contam Toxicol 100, 59–63 (2018). https://doi.org/10.1007/s00128-017-2221-4
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