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Stress-Induced Transcriptional Changes and DNA Damage Associated with Bis(2-ethylhexyl) Adipate Exposure in Zebrafish (Danio rerio) Larvae

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The present study evaluates potential toxic effects of bis(2-ethylhexyl) adipate (DEHA) plasticizer to larval (72 h post fertilization) zebrafish (Danio rerio) by analyzing changes in expression levels of stress-related genes (p53, rad51 and xrcc5) and assessing possible DNA damage of DEHA in larvae. The lethal concentration for 50% mortality (LC50) in larval zebrafish exposed for 96 h to 0–200 mg L−1 DEHA was 89.9 ± 8.03 mg L−1. A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed for 96 h to DEHA. There were some significant differences in induction of stress-related genes in larvae exposed to DEHA relative to control.

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This project was funded by Recep Tayyip Erdoğan University, Scientific Research Projects Fund (Project No. 2015.53001.103.01.02). The information contained in this article was extracted from a master’s thesis by the author, Serap Terzi.

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Correspondence to Halis Boran.

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Boran, H., Terzi, S. Stress-Induced Transcriptional Changes and DNA Damage Associated with Bis(2-ethylhexyl) Adipate Exposure in Zebrafish (Danio rerio) Larvae. Bull Environ Contam Toxicol 99, 308–314 (2017).

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