Stress-Induced Transcriptional Changes and DNA Damage Associated with Bis(2-ethylhexyl) Adipate Exposure in Zebrafish (Danio rerio) Larvae

  • Halis BoranEmail author
  • Serap Terzi


The present study evaluates potential toxic effects of bis(2-ethylhexyl) adipate (DEHA) plasticizer to larval (72 h post fertilization) zebrafish (Danio rerio) by analyzing changes in expression levels of stress-related genes (p53, rad51 and xrcc5) and assessing possible DNA damage of DEHA in larvae. The lethal concentration for 50% mortality (LC50) in larval zebrafish exposed for 96 h to 0–200 mg L−1 DEHA was 89.9 ± 8.03 mg L−1. A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed for 96 h to DEHA. There were some significant differences in induction of stress-related genes in larvae exposed to DEHA relative to control.


Bis(2-ethylhexyl) adipate Gene expression Genotoxicity Acute toxicity Zebrafish 



This project was funded by Recep Tayyip Erdoğan University, Scientific Research Projects Fund (Project No. 2015.53001.103.01.02). The information contained in this article was extracted from a master’s thesis by the author, Serap Terzi.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflicts of interest in the present work.


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© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Faculty of FisheriesRecep Tayyip Erdoğan UniversityRizeTurkey

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