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Stress-Induced Transcriptional Changes and DNA Damage Associated with Bis(2-ethylhexyl) Adipate Exposure in Zebrafish (Danio rerio) Larvae

  • Halis BoranEmail author
  • Serap Terzi
Article

Abstract

The present study evaluates potential toxic effects of bis(2-ethylhexyl) adipate (DEHA) plasticizer to larval (72 h post fertilization) zebrafish (Danio rerio) by analyzing changes in expression levels of stress-related genes (p53, rad51 and xrcc5) and assessing possible DNA damage of DEHA in larvae. The lethal concentration for 50% mortality (LC50) in larval zebrafish exposed for 96 h to 0–200 mg L−1 DEHA was 89.9 ± 8.03 mg L−1. A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed for 96 h to DEHA. There were some significant differences in induction of stress-related genes in larvae exposed to DEHA relative to control.

Keywords

Bis(2-ethylhexyl) adipate Gene expression Genotoxicity Acute toxicity Zebrafish 

Notes

Acknowledgements

This project was funded by Recep Tayyip Erdoğan University, Scientific Research Projects Fund (Project No. 2015.53001.103.01.02). The information contained in this article was extracted from a master’s thesis by the author, Serap Terzi.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflicts of interest in the present work.

References

  1. Abdul Rahman MB, Chaibakhsh N, Basri M, Salleh A, Abdul Rahman RNZR (2009) Application of artificial neural network for yield prediction of lipase-catalyzed synthesis of dioctyl adipate. Appl Biochem Biotechnol 158:722–735CrossRefGoogle Scholar
  2. Adams WJ, Biddinger GR, Robillard KA, Gorsuch JW (1995) A summary of the acute toxicity of 14 phthalate esters to representative aquatic organisms. Environ Toxicol Chem 14:1569–1574CrossRefGoogle Scholar
  3. Bladen CL, Lam WK, Dynan WS, Kozlowski DJ (2005) DNA damage response and Ku80 function in the vertebrate embryo. Nucleic Acids Res 33:3002–3010CrossRefGoogle Scholar
  4. Boran H, Ulutas G (2016) Genotoxic effects and gene expression changes in larval zebrafish after exposure to ZnCl2 and ZnO nanoparticles. Dis Aquat Org 117:205–214CrossRefGoogle Scholar
  5. Cheng R, Ford BL, O’Neal PE, Mathews CZ, Bradford CS, Thongtan T, Barnes DW, Hendricks JD, Bailey GS (1997) Zebrafish (Danio rerio) p53 tumor suppressor gene: cDNA sequence and expression during embryogenesis. Mol Mar Biol Biotechnol 6:88–97Google Scholar
  6. Chingin K, Chen HW, Gamez G, Zhu L, Zenobi R (2009) Detection of diethyl phthalate in perfumes by extractive electrospray ionization mass spectrometry. Anal Chem 81:123–129CrossRefGoogle Scholar
  7. Collins AR (2009) Investigating oxidative DNA damage and its repair using the comet assay. Mutat Res 681:24–32CrossRefGoogle Scholar
  8. Demir APT, Ulutan S (2013) Migration of phthalate and non-phthalate plasticizers out of plasticized PVC films into air. J Appl Polym Sci 128:1948–1961Google Scholar
  9. European Commission Joint Research Centre (2000) IUCLID CD-ROM, year 2000 edn. Public data on high volume chemicals. EUR 19559 EN. European Chemical Bureau, HelsinkiGoogle Scholar
  10. Felder JD, Adams WJ, Saeger VW (1986) Assessment of the safety of dioctyl adipate in freshwater environments. Environ Toxicol Chem 5:777–784CrossRefGoogle Scholar
  11. Francisco JLJ, Soledad R, Dolores P (2005) Determination of phthalate esters in sewage by hemimicelles-based solid-phase extraction and liquid chromatography-mass spectrometry. Anal Chim Acta 551:142–149CrossRefGoogle Scholar
  12. Horn O, Nalli S, Coope D, Nicell J (2004) Plasticizer metabolites in the environment. Water Res 38:3693–3698CrossRefGoogle Scholar
  13. Kondolot M, Ozmert EN, Asci A, Erkekoglu P, Oztop DB, Gumus H, Kocer-Gumusel B, Yurdakok K (2016) Plasma phthalate and bisphenol a levels and oxidant-antioxidantstatus in autistic children. Environ Toxicol Pharm 43:149–158CrossRefGoogle Scholar
  14. Liao W, McNutt MA, Zhu WG (2009) The comet assay: a sensitive method for detecting DNA damage in individual cells. Methods 48:46–53CrossRefGoogle Scholar
  15. Mathieu-Denoncourt J, Wallace SJ, de Solla SR, Langlois VS (2015) Plasticizer endocrine disruption: highlighting developmental and reproductive effects in mammals and non-mammalian aquatic species. Gen Comp Endocrinol 219:74–88CrossRefGoogle Scholar
  16. Mortensen GK, Main KM, Andersson AM, Leers H, Skakkebæk NE (2005) Determination of phthalate monoesters in human milk, consumer milk, and infant formula by tandem mass spectrometry (LC–MS/MS). Anal Bioanal Chem 382:1084–1092CrossRefGoogle Scholar
  17. Quinn-Hosey KM, Roche JJ, Fogarty AM, Brougham CA (2012) Screening for genotoxicity and oestrogenicity of endocrine disrupting chemicals in vitro. J Environ Prot 3:902–914CrossRefGoogle Scholar
  18. Reinardy HC, Dharamshi J, Jha AN, Henry TB (2013) Changes in expression profiles of genes associated with DNA repair following induction of DNA damage in larval zebrafish Danio rerio. Mutagenesis 28:601–608CrossRefGoogle Scholar
  19. Robertson GN, McGee CA, Dumbarton TC, Croll RP, Smith FM (2007) Development of the swimbladder and its innervation in the zebrafish, Danio rerio. J Morphol 268:967–985CrossRefGoogle Scholar
  20. Schmid P, Lorenz A, Hameister H, Montenarh M (1991) Expression of p53 during mouse embryogenesis. Development 113:857–865Google Scholar
  21. Staples CA, Peterson DR, Parkerton TF, Adams WJ (1997) The environmental fate of phthalate esters: a literature review. Chemosphere 35:667–749CrossRefGoogle Scholar
  22. Stuer-Lauridsen F, Mikkelsen S, Havelund S, Birkved M, Hansen LP (2001) COWI Consulting engineers and planners AS, environmental project No. 590: environmental and health assessment of alternatives to phthalates and to flexible PVC. Dan Environ Prot Agency. http://www2.mst.dk/udgiv/Publications/2001/87-7944-407-5/pdf/87-7944-408-3.pdf. Accessed 10 Apr 2017
  23. Suggatt RH, Foote K (1981) Comprehensive review of acute aquatic toxicity data on phthalate esters. Contract SRC TR 81–537. Syracuse Research Corporation, Final Report. Syracuse Research Corporation, SyracuseGoogle Scholar
  24. Thisse C, Neel H, Thisse B, Daujat S, Piette J (2000) The Mdm2 gene of zebrafish (Danio rerio): preferential expression during development of neural and muscular tissues, and absence of tumor formation after overexpression of its cDNAduring early embryogenesis. Differentiation 66:61–70CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Faculty of FisheriesRecep Tayyip Erdoğan UniversityRizeTurkey

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