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Genotoxic Assessment of Some Inorganic Compounds in Desert Pupfish (Cyprinodon macularius) in the Evaporation Pond from a Geothermal Plant

Abstract

The frequency of micro nucleated erythrocytes in peripheral blood of the desert pupfish (Cyprinodon macularius) from a geothermal effluent pond is determined and compared to organisms kept in an aquarium. The frequency of micronucleated erythrocytes found in pupfish from the geothermal pond is 2.75 (±2.09) and only 0.44 (±0.52) in captivity organisms. Dissolved As in the ponds doubles the 340 µg L−1 US-EPA acute quality criteria for aquatic life and Hg equals the 1.77 µg L−1 chronic criteria. The organisms with high MNE also have significantly high Se, As and Hg concentrations in muscle and liver. Compared to international maximum allowable limits for fish consumption, there is 81× enrichment for Se, 6× for As and 5× for Hg. Although Se is not significantly enriched in water, it is likely that its bioaccumulation occurs via feeding of detritus. The desert pupfish has a significant resistance to extreme metal accumulations and to recover under unpolluted conditions.

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References

  1. Ahmed MK, Hullah AMM, Hossain MA, Arif M, Parvin E, Akter MS, Khan MS, Islam MM (2011) Assessing the genotoxic potentials of arsenic in tilapia (Oreochromis mossambicus) using alkaline comet assay and micronucleus test. Chemosphere 84:143–149

    CAS  Article  Google Scholar 

  2. Al-Sabti K (1994) Micronuclei induced by selenium, mercury, methylmercury and their mixtures in binucleated blocked fish erythrocyte cells. Mutat Res 320:157–163

    CAS  Article  Google Scholar 

  3. Al-Sabti K, Metcalfe CD (1995) Fish micronuclei for assessing genotoxicity in water. Mutat Res/Genet Toxicol 343:121–135

    CAS  Article  Google Scholar 

  4. Bosch AC, O’Neill B, Sigge GO, Kerwath SE, Hoffman LC (2016) Heavy metals in marine fish meat and human health: a review. J Sci Food Agric 96:32–48

    CAS  Article  Google Scholar 

  5. Carrola J, Santos N, Rocha MJ, Fontainhas FA, Pardal MA, Monteiro RAF, Rocha E (2014) Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal. Environ Sci Pollut Res 21:6057–6068

    CAS  Article  Google Scholar 

  6. Christie NT, Costa M (1983) In vitro assessment of the toxicity of metal compounds: III. Effects of metals on DNA structure and function in intact cells. Biol Trace Elem Res 5:55–71

    CAS  Article  Google Scholar 

  7. Cockell KA, Bettger WJ (1993) Investigations of the gall bladder pathology associated with dietary exposure to disodium arsenate hepahydrate in juvenile rainbow trout (Oncorhynchus mykiss). Toxicology 77:233–248

    CAS  Article  Google Scholar 

  8. Cross FA, Hardy HL, Jones NY, Barber RT (1973) Relation between total body weight and concentration of Mn, Fe, Zn, Cu and Hg in the blue fish (Pomatonus saltatrix) and a bathy dermersal fish (Antimora rostrata). Fish Res Bd Can 30:1287–1291

    CAS  Article  Google Scholar 

  9. Datta S, Debabrata G, Dhira RSh, Shelley B, Shibnath M (2009) Chronic exposure to low concentration of arsenic is immunotoxic to fish: role of head kidney macrophages as biomarkers of arsenic toxicity to Clarias batrachus. Aquat Toxicol 92:86–94

    CAS  Article  Google Scholar 

  10. De Flora S, Vigano L, D’Agostini F, Camoirano A, Bagnasco M, Bennicelli C, Melodia F, Arillo A (1993) Multiple genotoxicity biomarkers in fish exposed in situ to polluted river water. Mutat Res 319:167–177

    Article  Google Scholar 

  11. Elvira B (1995) Conservation status of endemic freshwater fish in Spain. Biol Conserv 72:129–13

    Article  Google Scholar 

  12. Fangue NA, Flaherty KE, Rummer JL, Cole G, Hansen KS, Hinote R, Noel BL, Wallman H, Bennett WA (2001) Temperature and hypoxia tolerance of selected fishes from a hyperthermal rockpool in the Dry Tortugas, with notes on diversity and behavior. Caribb J Sci 37(1–2): 81–87

    Google Scholar 

  13. García-Hernández J, Glenn EP, Flessa K (2013) Identification of chemicals of potential concern (COPECs) in anthropogenic wetlands of the Colorado River delta. Ecol Eng 59:52–60

    Article  Google Scholar 

  14. Gill GA, Bruland KW (1990) Mercury speciation in surface freshwater systems in California and other areas. Environ Sci Technol 24(9):1392–1399

    CAS  Article  Google Scholar 

  15. Heddle JA (1973) A rapid in vivo test for chromosomal damage. Mutat Res 18:307–317

    Article  Google Scholar 

  16. Hernández JMC (2009) Zonas de alteración hidrotermal y condiciones actuales del yacimiento: un enfoque para determinar zonas productoras al oriente del campo geotérmico de Cerro Prieto, BC. Geotermia 22(2):35–44

    Google Scholar 

  17. Kalay M, Ay O, Canli M (1999) Heavy metal concentrations in fish tissues from the Northeast Mediterranean Sea. Bull Environ Contam Toxicol 63:673–681

    CAS  Article  Google Scholar 

  18. Licata P, Tromberra D, Cristani M, Naccari C, Martino D, Calo M (2005) Heavy metals in liver and muscle of bluefin tuna (Thunnus thynnus) caught in the straits of Messina (Sicily, Italy). Environ Monit Assess 107:239–248

    CAS  Article  Google Scholar 

  19. McFarlane GA, Franxin WG (1980) An examination of Cd, Cu and Hg concentrations in livers of northern pike, Esox lucius and white sucker, Catostomus commersoni, from five lakes near a base metal smelter at Flin Flon, Manitoba. Can J Fish Aquat Sci 37:1573–1578

    CAS  Article  Google Scholar 

  20. Metcalfe CD (1988) Induction of micronuclei and nuclear abnormalities in the erythrocytes of mudminnows (Umbra limi) and brown bullheads (lctalurus nebulosus). Bull Environ Contam Toxicol 40:489–495

    CAS  Article  Google Scholar 

  21. Miller LL, Rasmussen JB, Palace VP, Sterling G, Hontela A (2013) Selenium bioaccumulation in stocked fish as an indicator of fishery potential in pit lakes on reclaimed coal mines in Alberta Canada. Environ Manag 52(1):72–84

    CAS  Article  Google Scholar 

  22. NOM (1997) Norma Oficial Mexicana, NOM-001-ECOL-1996. Establece los límites máximos permisibles de contaminantes en las descargas residuals en aguas y bienes nacionales. http://dof.gob.mx/nota_detalle.php?codigo=4863829&fecha=06/01/1997

  23. NOM (2011) Norma Oficial Mexicana, NOM-242-SSA1-2009. Productos y servicios. Productos de la pesca frescos, refrigerados, congelados y procesados. http://dof.gob.mx/nota_detalle.php?codigo=5177531&fecha=10/02/2011

  24. Ruiz-Campos G, Camarena-Rosales F, González-Acosta AF, Maeda-Martínez AM, García de León FJ, Varela-Romero A, Andreu-Soler A (2014) Estatus actual de conservación de seis especies de peces dulceacuícolas de la Península de Baja California, México. Rev Mex Biodivers 85:1235–1248

    Article  Google Scholar 

  25. Sabadell JE, Axtmann R (1975) Heavy metal contamination from geothermal sources. Environ Health Perspect 12:1–7

    CAS  Article  Google Scholar 

  26. Schmid W (1975) The micronucleus test. Mutat Res 31:9–15

    CAS  Article  Google Scholar 

  27. Skinner BJ, White DE, Rose HJ, Mays RE (1967) Sulfides associated with the Salton Sea geothermal brine. Econ Geol 62:316–333

    CAS  Article  Google Scholar 

  28. Soldatov AA (2005) Peculiarities of organization and functioning of the fish red blood system. J Evol Biochem Physiol 41:272–281

    CAS  Article  Google Scholar 

  29. Udroiu I (2006) The micronucleus test in piscine erythrocytes. Aquat Toxicol 79:201–204

    CAS  Article  Google Scholar 

  30. Ueda T, Hayashi M, Ohtsuka Y, Nakamura T, Kobayashi J, Sofuni T (1992) A preliminary study of the micronucleus test by acridin orange fluorescent staining compared with chromosomal aberration test using fish erythropoietic and embryonic cells. Water Sci Technol 25(11):235–240

    CAS  Google Scholar 

  31. USEPA (2000) Guidance for assessing chemical contaminant data for use in fish advisories volume 1: fish sampling and analysis (EPA-823-B-00-007). United States Environmental Protection Agency, Office of Science and Technology-Office of Water

  32. USEPA (2014) External peer review draft aquatic life ambient water quality criterion for selenium – freshwater, United States Environmental Protection Agency, Office of Water, Office of Science and Technology, (EPA-820-F-14-005) Washington, D.C

  33. USEPA (2016) Aquatic life quality criterium for Selenium—fresh water. United States Environmental Protection Agency, Office of Water, (EPA-822-R-16-06) Washington, DC

  34. USEPA (2017) National recommended water quality criteria—aquatic life criteria table. https://www.epa.gov/wqc/national-recommended-water-quality-criteria-aquatic-life-criteria-table

  35. USFWS (1987) Aquatic cycling of selenium: implications for fish and wildlife, fish and wildlife leaflet. United State Department of the Interior Fish and Wildlife Service 12, Washington DC

  36. USFWS (1993) Desert Pupfish (Cyprinodon macularius) Recovery Plan, Unpublished report to U.S. Fish and Wildlife Service, Region 2, Albuquerque, New México, with assistance from Arizona Game and Fish Department and Tonto National Forest 129, Washington DC

  37. USFWS (2010) Desert pupfish (Cyprinodon macularius) 5-year review: summary and evaluation. Phoenix, United States Fish and Wildlife Service 42, Washington, DC

  38. Varela-Romero A, Ruiz-Campos G, Yépiz-Velázquez LM, Alaníz-García, J (1999) Evaluación de la situación actual de las poblaciones del pez cachorrito del desierto (Cyprinodon macularius macularius) en la Cuenca del Bajo Río Colorado, Sonora-Baja California, México. Universidad de Sonora, Informe final SNIB-CONABIO proyecto No. H126, México DF

  39. Varela-Romero A, Ruiz-Campos G, Yépiz-Velázquez LM, Alaníz-García J (2002) Distribution, habitat and conservation status of desert pupfish (Cyprinodon macularius) in the Lower Colorado River Basin, Mexico. Rev Fish Biol Fish 12:157–165

    Article  Google Scholar 

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Acknowledgements

Funding for this project by CONACYT Grant (368374) to first author. We thank K. Juárez Moreno (Center of Nanoscience and Nanotechnology, UNAM) for her training and help with the fluorescence microscope; O. Torres Bugarín (International Program of the Faculty of Medicine, UAG) for her assistance in the micronucleus technique; L. Lares Reyes (CICESE) for access to the lyophilizer; T. Kretzschmar and E. Iñiguez (CeMIE-Geo/CICESE) for part of the water analyses. The Dirección General de Vida Silvestre of Mexico for sampling permit SGPA/DGVS 09605/12. We are grateful for the helpful comments by two anonymous reviewers and the editorial board editor.

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Correspondence to Luis Walter Daesslé.

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Flores-Galván, M., Arellano-García, E., Ruiz-Campos, G. et al. Genotoxic Assessment of Some Inorganic Compounds in Desert Pupfish (Cyprinodon macularius) in the Evaporation Pond from a Geothermal Plant. Bull Environ Contam Toxicol 99, 218–223 (2017). https://doi.org/10.1007/s00128-017-2114-6

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Keywords

  • Micronuclei
  • Erythrocytes
  • Fish
  • Mercury
  • Arsenic
  • Selenium