When ionic liquids (ILs) first came into use, we thought that they were safe. However, upon further investigation, researchers found that ILs are not harmless. In this study, the model soil organism, earthworms (Eisenia foetida), were used to study the acute toxicity of imidazole nitrate ionic liquids with varying chain lengths from 2 to 12. The experiment used two different methods, a filter paper contact test (48 h) and an artificial soil test (14 days), to determine the toxicity. These results demonstrated that the toxicity increased with the length of carbon chains until C8 and that the cut-off effect occurred at 1-octyl-3-methyl imidazole nitrates.Then, the toxicity began to increase again. At the same time, the concentrations of [C10mim]NO3 and [C12mim]NO3 were determined by high performance liquid chromatography and demonstrated that ILs were stable throughout the experiment. The present study revealed the acute toxicity of ILs with varying chain lengths.
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The present study was supported by grants from the National Key Research and Devel opment Plan (No. 2016YFD0800202) and the National Natural Science Foundation of China (Nos. 21377075, 41671320). All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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Shao, Y., Du, Z., Zhang, C. et al. Acute Toxicity of Imidazole Nitrate Ionic Liquids with Varying Chain Lengths to Earthworms (Eisenia foetida). Bull Environ Contam Toxicol 99, 213–217 (2017). https://doi.org/10.1007/s00128-017-2082-x
- Filter paper contact
- Artificial soil
- Cut-off effect
- Concentration measure