Remediation of Petroleum-contaminated Soil Using Bulrush Straw Powder, Biochar and Nutrients
- 308 Downloads
The aim of this study was to determine the remediation efficiency of petroleum-contaminated soil from an oilfield using different types of remediation treatments under laboratory conditions. Compared with unamended soil as the control treatment (T1), soil samples were amended with bulrush straw powder (T2), with biochar alone (T3) and in combination with nutrients (nitrogen and phosphorus) (T4). The remediation experiment was carried out for 8 weeks. The extent of hydrocarbon degradation was monitored gravimetrically, and the residual oil fractions were analyzed by gas chromatography. The characteristics of the polluted soil (water-holding capacity and nutrients) were improved significantly by biochar addition (p < 0.05). The total microbial count increased significantly in the treatment containing biochar and added nutrients (t = 23.429, p = 0.002). The degradation of total petroleum hydrocarbons (TPH) and the main hydrocarbon fractions was higher in T3 and T4, especially in T4, than in T1 and T2. The intensities of the n-alkane fraction, C27–C29 steranes and C33–C35 homohopanes were efficiently decreased in T4 compared to the other treatments. According to the results, petroleum-contaminated soil can be remediated efficiently by adding biochar and nutrients simultaneously, and this combination of remediation was superior to that observed with added bulrush straw powder.
KeywordsPetroleum hydrocarbons Biochar Nutrients Microorganisms
This work was supported by the National Natural Science Foundation of China (No. 41571464), Program of Liaoning Education Department (No. LR2015035) and Environmental Science and Engineering Innovation Team Programme of Liaoning Shihua University (-11).
- Dindar E, Şağban FOT, Başkaya HS (2013) Bioremediation of petroleum-contaminated soil. J Bio Environ Sci 7:39–47Google Scholar
- Gallotta FDC, Christensen JH (2012) Source identification of petroleum hydrocarbons in soil and sediments from Iguaçu River watershed, Paraná, Brazil using the CHEMSIC method (CHEMometric analysis of selected Ion chromatograms). J Chromatogr A 1235:149–158. doi: 10.1016/j.chroma.2012.02.041 CrossRefGoogle Scholar
- Karamalidis AK, Evangelou AC, Karabika E, Koukkou AI, Drainas C, Voudrias EA (2010) Laboratory scale bioremediation of petroleum-contaminated soil by indigenous microorganisms and added Pseudomonas aeruginosa strain Spet. Bioresour Technol 101:6545–6552. doi: 10.1016/j.biortech.2010.03.055 CrossRefGoogle Scholar
- Meynet P, Moliterni E, Davenport RJ, Sloan WT, Camacho JV, Werner D (2014) Predicting the effects of biochar on volatile petroleum hydrocarbon biodegradation and emanation from soil: a bacterial community finger-print analysis inferred modelling approach. Soil Biol Biochem 68:20–30. doi: 10.1016/j.soilbio.2013.09.015 CrossRefGoogle Scholar
- Quilliam RS, Marsden KA, Gertler C, Rousk J, DeLuca TH, Jones DL (2012) Nutrient dynamics, microbial growth and weed emergence in biochar amended soil are influenced by time since application and reapplication rate. Agr Ecosyst Environ 158:192–199. doi: 10.1016/j.agee.2012.06.011 CrossRefGoogle Scholar