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Design and Development of Acetylthiocholine Electrochemical Biosensor Based on Zinc Oxide–Cerium Oxide Nanohybrid Modified Platinum Electrode

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Abstract

Acetylcholinesterase (AChE) enzyme has been predominantly used for the detection of pesticides and metal ions. But, these sensors respond to pesticides as well as metal ions at certain concentration, which results in poor selectivity. Hence in this work, the amount of thiocholine produced during AChE inhibition has been estimated to detect the residual activity of AChE enzyme in-turn to enhance the efficiency of the biosensor. In this context, Pt/ZnO–CeO2/AChE/Chitosan based biosensor has been developed for sensitive voltammetric quantification of thiocholine in AChE. The sensor exhibited enhanced electron transfer rate, good conductivity and biocompatibility. Both the intrinsic and extrinsic parameters were simultaneously optimized using second order polynomial regression to get the best conditions for ATCh determination. Under optimized experimental conditions, the redox peak current was linear over the concentration range of 0.1–1.5 mM with detection and quantification limit of 0.05 and 0.15 μM respectively and the sensitivity of 1.47 μA mM−1.

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Acknowledgements

The authors are grateful to the Department of Science & Technology, New Delhi for their financial support (DST/TM/WTI/2K14/197(a)(G)), (SR/FST/ETI-284/2011 (C)), (SR/FST/LSI-453/2010) and (SR/NM/PG-16/2007). The research was supported by Professor T. R. Rajagopalan research fund, SASTRA University. We also acknowledge SASTRA University, Thanjavur for extending infrastructural support to carry out the study.

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Authors and Affiliations

  1. Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India

    Manju Bhargavi Gumpu, Noel Nesakumar, Srinidhi Nagarajan, Sadhana Ramanujam, K. Jayanth Babu & John Bosco Balaguru Rayappan

  2. School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India

    Manju Bhargavi Gumpu, Srinidhi Nagarajan, Sadhana Ramanujam, K. Jayanth Babu & John Bosco Balaguru Rayappan

  3. Centre for Nanotechnology & Advanced Biomaterials, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India

    Manju Bhargavi Gumpu, Noel Nesakumar, Uma Maheswari Krishnan & John Bosco Balaguru Rayappan

  4. School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India

    Noel Nesakumar & Uma Maheswari Krishnan

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  1. Manju Bhargavi Gumpu
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  2. Noel Nesakumar
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  6. K. Jayanth Babu
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  7. John Bosco Balaguru Rayappan
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Correspondence to John Bosco Balaguru Rayappan.

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Gumpu, M.B., Nesakumar, N., Nagarajan, S. et al. Design and Development of Acetylthiocholine Electrochemical Biosensor Based on Zinc Oxide–Cerium Oxide Nanohybrid Modified Platinum Electrode. Bull Environ Contam Toxicol 98, 662–671 (2017). https://doi.org/10.1007/s00128-017-2045-2

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  • DOI: https://doi.org/10.1007/s00128-017-2045-2

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