Time-Dependent Toxicity Responses in Daphnia magna Exposed to CuO and ZnO Nanoparticles

  • Soyoun Kim
  • Palas Samanta
  • Jisu Yoo
  • Woo-Keun Kim
  • Jinho JungEmail author


Aggregation and dissolution of CuO and ZnO nanoparticles (NPs) increased with increasing exposure time (24, 48, and 72 h). Acute toxicity of CuO NPs to Daphnia magna also increased significantly with increasing exposure time (p < 0.05), whereas exposure time did not significantly affect acute toxicity of ZnO NPs. The dissolved Cu concentration of CuO NPs was much lower than the median effective concentration (EC50) value (44 μg L−1 at 72 h), implying that the increase in acute toxicity was caused by particles rather than by dissolved ions. However, the dissolved Zn concentration of ZnO NPs was higher than the EC50 value (600 μg L−1 at 72 h), suggesting this acute toxicity may be caused by dissolved ions. Moreover, CuO NPs induced greater lipid peroxidation than Cu ions did at an exposure time of 72 h, whereas converse results were observed for ZnO NPs.


Acute toxicity Copper Daphnid Nanotoxicology Oxidative stress Zinc 



This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (No. NRF-2016R1A2B4016299), and by the Korean Ministry of Environment as “Development of integrated model for climate change impact and vulnerability assessment”.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Soyoun Kim
    • 1
  • Palas Samanta
    • 1
  • Jisu Yoo
    • 1
  • Woo-Keun Kim
    • 2
  • Jinho Jung
    • 1
  1. 1.Division of Environmental Science and Ecological EngineeringKorea UniversitySeoulRepublic of Korea
  2. 2.System Toxicology Research CenterKorea Institute of ToxicologyDaejeonRepublic of Korea

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