Abstract
Two-stage in situ wetlands (two vertical flow constructed wetlands in parallel and a horizontal flow constructed wetland) were constructed for studying domestic wastewater purification and the correlations between contaminant removal and plant and soil enzyme activities. Results indicated the removal efficiency of NH4 + and NO3 − were significantly correlated with both urease and protease activity, and the removal of total phosphorus was significantly correlated with phosphatase activity. Chemical oxygen demand removal was not correlated with enzyme activity in constructed wetlands. Plant root enzyme (urease, phosphatase, protease and cellulose) activity correlation was apparent with all contaminant removal in the two vertical flow constructed wetlands. However, the correlation between the plant root enzyme activity and contaminant removal was poor in horizontal flow constructed wetlands. Results indicated that plant roots clearly played a role in the removal of contaminants.
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References
Cooper PF, Job GD, Green MB (1996) Reed beds and constructed wetlands for wastewater treatment. WRc Publications, Medmenham
Duarte B, Reboreda R, Cacador I (2008) Seasonal variation of extracellular enzymatic activity (EEA) and its influence on metal speciation in a polluted salt marsh. Chemosphere 73:1056–1063
Haberl R, Perfler R (1991) Nutrient removal in a reed bed system. Water Sci Technol 23:729–737
Huang L, Gao X, Liu M, Du G, Guo JS, Theoneste N (2012) Correlation among soil microorganisms, soil enzyme activities, and removal rates of pollutants in three constructed wetlands purifying micro-polluted river water. Ecol Eng 46:98–106
Jones DL, Hodge A, Kuzyakov Y (2004) Plant and mycorrhizal regulation of rhizodeposition. New Phytol 163:459–480
Kandeler E, Luxhùi J, Tscherko D, Magid J (1999) Xylanase, invertase and protease at the soil-litter interface of a loamy sand. Soil Biol Biochem 31:1171–1179
Keith RE, Hana C, Katerina Z, Hana S (2006) Plant growth and microbial processes in a constructed wetland planted with Phalaris arundinacea. Ecol Eng 27:153–165
Klose S, Tabatabai MA (2000) Urease activity of microbial biomass in soils as affected by cropping systems. Biol Fertil Soils 31:191–199
Kong L, Wang YB, Zhao L, Chen ZH (2009) Enzyme and root activities in surface flow constructed wetlands. Chemosphere 76:601–608
Liang W, Wu Z, Cheng S, Zhou Q, Hu H (2003) Roles of substrate microorganisms and urease activities in wastewater purification in a constructed wetland system. Ecol Eng 21:191–195
Margesin R, Zimmerbauer A, Schinner F (2000) Monitoring of bioremediation by soil biological activities. Chemosphere 40:339–346
Müller N, Bordusa F (2000) Assay of diverse protease activities on the basis of a small synthetic substrate. Anal Biochem 286:86–90
Neori A, Reddy KR, Ciskova-Koncalova H, Agami M (2000) Bioactive chemicals and biological–biochemical activities and their functions in rhizospheres of wetland plants. Bot Rev 66:351–378
Niemi RM, Vepsalainen M, Wallenius K, Simpanen S, Alakukku L, Pietola L (2005) Temporal and soil depth-related variation in soil enzyme activities and inroot growth of red clover (Trifolium pratense) and timothy (Phleum pratense) in the field. Appl Soil Ecol 30:113–125
Oehl F, Frossard E, Fliessbach A, Dubois D, Oberson A (2004) Basal organic phosphorus mineralization in soils under different farming systems. Soil Biol Biochem 36:667–675
Ovez B, Ozgen S, Yuksel M (2006) Biological denitrification in drinking water using Glycyrrhiza glabra and Arunda donax as the carbon source. Process Biochem 41:1539–1544
Reboreda R, Cacador I (2008) Enzymatic activity in the rhizosphere of Spartina maritima: potential contribution for phytoremediation of metals. Mar Environ Res 65:77–84
Schinner F, von Mersi W (1990) Xylanase-, CM-cellulase- and invertase activity in soil: an improved method. Soil Biol Biochem 22:511–515
Semenov AM, Batomunkueva BP, Nizovtseva DV, Panikov NS (1996) Method of determination of cellulase activity in soils and in microbial cultures, and its calibration. J Microbiol Methods 24:259–267
Shackle VJ, Freeman C, Reynolds B (2000) Carbon supply and the regulation of enzyme activity in constructed wetlands. Soil Bio Biochem 32:1935–1940
State Environmental Protection Administration of China (2002) Methods for water analysis, 4th edn. Environment Science Press, Beijing, pp 200–285 (in Chinese)
Sundberg C, Tonderski K, Lindgren PE (2007) Potential nitrification and denitrification and the corresponding composition of the bacterial communities in a compact constructed wetland treating landfill leachates. Water Sci Technol 56:159–166
Truu M, Juhanson J, Truu J (2009) Microbial biomass and activity and community composition in constructed wetlands. Sci Total Environ 407:3958–3971
Vymazal J (2007) Removal of nutrients in various types of constructed wetlands. Sci Total Envriron 380:48–65
Vymazal J (2014) Constructed wetlands for treatment of industrial wastewaters. Review 73:724–751
Vymazal J, Krǒpfelová L (2008) Wastewater treatment in constructed wetlands with horizontal subsurface flow. Springer, Dordrecht
Zhang BJ, Bai XL, He KL, Feng QY, Cheng YJ (2007) Distribution status of soil microbes and enzyme activity in bio-salver. Environ Sci Technol 30:26–28
Zhang S, Zhou Q, Xu D, He F, Cheng S, Liang W, Du C, Wu Z (2010) Vertical-flow constructed wetlands applied in a recirculating aquaculture system for channel catfish culture: effects on water quality and zooplankton. Polish J Environ Stud 19:1063–1070
Acknowledgments
This work has been supported jointly by the National Natural Science for Creative Research Groups of China (No. 51421006); the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT13061); the National Natural Science Foundation (Grant Nos. 51109061, 41373111); the National Science Funds for Distinguished Young Scholars (Grant No. 51225901); the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD); the Project Funded by Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (TAPP).
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Ni, L., Xu, J., Chu, X. et al. Correlation Among Soil Enzyme Activities, Root Enzyme Activities, and Contaminant Removal in Two-Stage In Situ Constructed Wetlands Purifying Domestic Wastewater. Bull Environ Contam Toxicol 97, 131–137 (2016). https://doi.org/10.1007/s00128-016-1844-1
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DOI: https://doi.org/10.1007/s00128-016-1844-1