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Evaluation of the Methods to Predict Total Serum Lipids and to Determine 22 POPs in the Serum of Pregnant Women

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Abstract

The objectives of this study were to determine the suitability of total serum lipid (TSL) concentrations, which were calculated by three different formulae, for pregnant women and to optimize the improved matrix solid-phase dispersion (MSPD) method. The results showed that the TSL predicted by the three formulae were significantly correlated to our measured values (sums of the total cholesterol, triglycerides and phospholipids contents). In particular, one of formulae was the most statistically suitable for pregnant women. Meanwhile, an improved MSPD method was developed to extract 22 POPs. The developed MSPD method was compared with SPE and EPA (8081 & 8082) to evaluate the performance of each extract method. The method validation showed that the results obtained using the improved MSPD method were closest to the actual concentration (adjusted by lipids), and the dispersion of the data was minimal.

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Acknowledgments

We thank our colleagues at the Cixi CDC and CIXI Maternal and Child Care Service Centre for sampling assistance and total cholesterol, triglyceride concentration data. This study was supported by the Zhejiang Province Medical and Health Science Research Foundation (No. 2011RCA031) and the Agriculture and Social Development Funds of Ningbo, China (No. 2012C0030). To obtain ethical approval, all pregnant women in the study were informed that their blood would be employed in the chemical testing and agreed to participate in our research.

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Authors and Affiliations

  1. Jiangbei Center for Disease Control and Prevention, Ningbo, 315020, China

    Peng Zhao, Lixiao Qiu, Fenfen Xu & Yifeng Wu

  2. Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, China

    Jiawei Shi, Jige Li & Micong Jin

  3. Cixi Center for Disease Control and Prevention, Ningbo, 315020, China

    Wenjie Gong

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  1. Peng Zhao
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  2. Jiawei Shi
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  3. Lixiao Qiu
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  4. Jige Li
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  6. Fenfen Xu
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  7. Wenjie Gong
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  8. Yifeng Wu
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Correspondence to Jiawei Shi.

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Zhao, P., Shi, J., Qiu, L. et al. Evaluation of the Methods to Predict Total Serum Lipids and to Determine 22 POPs in the Serum of Pregnant Women. Bull Environ Contam Toxicol 97, 124–130 (2016). https://doi.org/10.1007/s00128-016-1807-6

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  • DOI: https://doi.org/10.1007/s00128-016-1807-6

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