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Effects of Barium Chloride Exposure on Hormones and Genes of the Hypothalamic–Pituitary–Gonad Axis, and Reproduction of Zebrafish (Danio rerio)


Adult zebrafish pairs were exposed to sub-lethal concentrations of BaCl2 for 21 days, and the effects on reproduction, sex steroid hormones, and transcription of the genes belonging to the hypothalamic–pituitary–gonad (HPG) axis were investigated. The adverse effects on performances of F1 generation were further examined with or without subsequent exposure to BaCl2. Egg production was significantly decreased, and parental exposure to BaCl2 resulted in lesser rates of hatching. In males, exposure to BaCl2 resulted in greater concentrations of E2 along with greater mRNA expression of cyp19a. The results demonstrated that BaCl2 could modulate gene transcriptions and hormone production of the HPG axis in a sex-dependent way, which could cause adverse effects on reproduction and the development of offspring.

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  1. Bjerselius R, Lundstedt-Enkel K, Olsén H, Mayer I, Dimberg K (2001) Male goldfish reproductive behaviour and physiology are severely affected by exogenous exposure to 17β-estradiol. Aquat Toxicol 53:139–152

    Article  CAS  Google Scholar 

  2. Cosmetic Ingredient Review (2014) Safety assessment of barium sulfate as used in cosmetics

  3. Devlin RH, Nagahama Y (2002) Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture 208:191–364

    Article  CAS  Google Scholar 

  4. Fenske M, Segner H (2004) Aromatase modulation alters gonadal differentiation in developing zebrafish (Danio rerio). Aquat Toxicol 67:105–126

    Article  CAS  Google Scholar 

  5. Hazardous Substances Data Bank (HSDB) (2014) Accessed 16 Jun 2014

  6. Hutchinson TH, Ankley GI, Segner H, Tyler CR (2006) Screening and testing for endocrine disruption in fish-biomarkers as “signposts”, not “traffic lights”, in risk assessment. Environ Health Perspect 114:106–114

    Article  Google Scholar 

  7. Ji K, Liu X, Lee S, Kang S, Kho Y, Giesy JP, Choi K (2013) Effects of non-steroidal anti-inflammatory drugs on hormones and genes of the hypothalamic–pituitary–gonad axis, and reproduction of zebrafish. J Hazard Mater 254–255:242–251

    Article  Google Scholar 

  8. Kang IJ, Yokata H, Oshima Y, Tsuruda Y, Hano T, Maeda M, Imada N, Tadokoro H, Honjo T (2003) Effects of 4-nonylphenol on reproduction of Japanese medaka, Oryzias latipes. Environ Toxicol Chem 22:2438–2445

    Article  CAS  Google Scholar 

  9. Kinnberg K, Korsgaard B, Bjerregaard P, Jespersen AS (2000) Effects of nonylphenol and 17β-estradiol on vitellogenin synthesis and testis morphology in male platyfish Xiphophorus maculates. J Exp Biol 203:171–181

    CAS  Google Scholar 

  10. Korea Consumer Agency (2013) Children’s face paint found to be toxic. Accessed 02 Jun 2015

  11. Liu X, Ji K, Jo A, Moon HB, Choi K (2013) Effects of TDCPP or TPP on gene transcriptions and hormones of HPG axis, and their consequences on reproduction in adult zebrafish (Danio rerio). Aquat Toxicol 134–135:104–111

    Google Scholar 

  12. Ma Y, Han J, Guo Y, Lam PKS, Wu RSS, Giesy JP, Zhang X, Zhou B (2012) Disruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol. Aquat Toxicol 106–107:173–181

    Article  Google Scholar 

  13. Miles-Richardson SR, Pierens SL, Nichols KM, Kramer VJ, Snyder SA, Render JA, Fitzgerald SD, Giesy JP (1999) Effects of waterborne exposure to 4-nonylphenol and nonylphenol ethoxylate on secondary sex characteristics and gonads of fathead minnows (Pimephales promelas). Environ Res 80:S122–S137

    Article  CAS  Google Scholar 

  14. Nagahama Y, Yamashita M (2008) Regulation of oocyte maturation in fish. Dev Growth Differ 50:S195–S219

    Article  CAS  Google Scholar 

  15. OECD (2009) OECD guideline for testing of chemicals, test no. 229: fish short term reproduction assay. Paris, France

  16. Sofikitis N, Giotitsas N, Tsounapi P, Baltogiannis D, Giannakis D, Pardalidis N (2008) Hormonal regulation of spermatogenesis and spermiogenesis. Steroid Biochem Mol Biol 109:323–330

    Article  CAS  Google Scholar 

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This study was supported by National Research Foundation of Korea (NRF; Project No. 2013R1A1A1061684).

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Correspondence to Kyunghee Ji.

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Kwon, B., Ha, N., Jung, J. et al. Effects of Barium Chloride Exposure on Hormones and Genes of the Hypothalamic–Pituitary–Gonad Axis, and Reproduction of Zebrafish (Danio rerio). Bull Environ Contam Toxicol 96, 341–346 (2016).

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  • Barium chloride
  • Endocrine disruption
  • HPG axis
  • Reproduction
  • Zebrafish