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Biomarkers of Cytotoxic, Genotoxic and Apoptotic Effects in Cyprinus carpio Exposed to Complex Mixture of Contaminants from Hospital Effluents

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Abstract

Hospital wastewater is an important source of emerging contaminants. Recent studies emphasize the importance of assessing the effects of mixtures of contaminants rather than environmental risk of their individual components, as well as the determination of intrinsic toxicity of wastewater. Mixtures of pollutants has possible interactions that have notable environmental side effects. The aim of this study is an attempt to characterize biomarkers in Cyprinus carpio related to the exposure to a complex mixture of contaminants found in hospital wastewater. Results of a particular hospital effluent show the presence of traces of heavy metals, high chlorine concentration and emerging contaminants such as non-steroidal anti-inflammatory drugs. The LC50 was of 5.49 % at 96 h. The cytotoxic, genotoxic and apoptotic biomarkers increase when fishes were exposed to wastewater (1/10 CL50) from hospital wastewater. This study emphasizes the importance of identifying and quantifying the effects of contaminants as pharmaceuticals, disinfectants and surfactants in order to design and implement an ecotoxicologycal plan.

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References

  • Ayllon F, Garcia-Vazquez E (2000) Induction of micronuclei and other nuclear abnormalities in European minnow Phoxinus phoxinus and mollie Poecilia latipinna: an assessment of the fish micronucleus test. Mutat Res 467(2):177–186. doi:10.1016/S1383-5718(00)00033-4

    Article  CAS  Google Scholar 

  • Boillot C, Bazin C, Tissot-Guerraz F, Droguet J, Perraud M, Cetre JC, Trepo D, Perrodin Y (2008) Daily physicochemical, microbiological and ecotoxicological fluctuations of a hospital effluent according to technical and care activities. Sci Total Environ 403:113–129

    Article  CAS  Google Scholar 

  • Bolognesi C, Hayashi M (2011) Micronucleus assay in aquatic animals. Mutagenesis 26(1):205–213. doi:10.1093/mutage/geq073

    Article  CAS  Google Scholar 

  • Buschini A, Martino A, Gustavino B, Monfrinotti M, Poli P, Rossi C, Santoro M, Dorr AJM, Rizzoni M (2004) Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization. Mutat Res 557:119–129

    Article  CAS  Google Scholar 

  • Emmanuel E, Perrodin Y, Keck G, Blanchard JM, Vermande P (2005) Ecotoxicological risk assessment of hospital wastewater: a proposed framework for raw effluents discharging into urban sewer network. J Hazard Mater 117(1):1–11

    Article  CAS  Google Scholar 

  • [EPA], Unite States Environmental Protection Agency. Aug 2014. http://www.epa.gov/ncea/bmds/dwnldu.html

  • Escher BI, Baumgartner R, Koller M, Treyer K, Lienert J, McArdell CS (2011) Environmental toxicology and risk assessment of pharmaceuticals from hospital wastewater. Water Res 45:75–92

    Article  CAS  Google Scholar 

  • Fan TJ, Han LH, Cong RS, Liang J (2005) Caspase family proteases and apoptosis. Acta Biochim Biophys Sin (Shanghai) 37(11):719–727

    Article  CAS  Google Scholar 

  • Gao D, Xu Z, Qiao P, Zhang SL, He P, Zhang X, Wan Y, Min W (2013) Cadmium induces liver cell apoptosis through caspase-3A activation in purse red common carp (Cyprinus carpio). PLoS One 8(12):e83423

    Article  Google Scholar 

  • García-Medina S, Núñez-Betancourt J, García-Medina A, Galar-Martínez M, Neri-Cruz N, Islas-Flores LM, Gómez-Oliván LM (2013) The relationship of cytotoxic and genotoxic damage with blood aluminum levels and oxidative stress induced by this metal in common carp (Cyprinus carpio) erythrocytes. Ecotoxicol Environ Saf 96:191–197

    Article  Google Scholar 

  • González-González ED, Gómez-Olivan LM et al (2014) Metals and nonsteroidal anti-inlfamatory pharmacueticals drug present in water from Madín Reservoir (México) induce oxidative stress in gill, blood and muscle of common carp (Cyprinus carpio). Arch Environ Contam Toxicol 67:281–295

    Article  Google Scholar 

  • Grisolia CK, Starling FL (2001) Micronuclei monitoring of fishes from Lake Paranoa, under influence of sewage treatment plant discharges. Mutat Res 491:39–44

    Article  CAS  Google Scholar 

  • Gronczewska J, Zietara MS, Biegniewska A, Skorkowski EF (2003) Enzyme activities in fish spermatozoa with focus on lactate dehydrogenase isoenzymes from herring Clupea harengus. Comp Biochem Physiol B: Biochem Mol Biol 134B:399–406

    Article  CAS  Google Scholar 

  • Islas-Flores H, Gómez-Oliván LM, Galar-Martínez M, Colín-Cruz A, Neri-Cruz N, García-Medina S (2013) Diclofenac-induced oxidative stress in brain, liver, gill and blood of common carp (Cyprinus carpio). Ecotoxicol Environ Saf 92:32–38

    Article  CAS  Google Scholar 

  • Jardim WF, Montgner CC, Pescara IC, Umbuzeiro GA, Di Dea AM, Eldridge AL, Sodré FF (2012) An integrated approach to evaluate emerging contaminants in drinking water. Sep Purif Technol 84(9):3–8

    Article  CAS  Google Scholar 

  • Jelic A, Petrovic M, Barceló D (2012) Pharmaceuticals in drinking water. Emerging organic contaminants and human health the handbook of environmental chemistry. Springer Link pp 47–70

  • Kortenkamp A, Backhaus T, Faust M (2009) State of the art on mixture toxicity. Report. Last Accessed 02 Sept 2014. http://ec.europa.eu/environment/chemicals/pdf/report_Mixture%20toxicity.pdf

  • Kosma CI, Lambropoulou DA, Albanis TA (2010) Occurrence and removal of PPCPs in municipal and hospital wastewaters in Greece. J Hazard Mater 179:804–817

    Article  CAS  Google Scholar 

  • Kümmerer K (2002) Drugs in the environment: emission of drugs, diagnostic aids and disinfectants into wastewater by hospitals in relation to other sources: a review. Chemosphere 48(3):383

    Article  Google Scholar 

  • Lapworth DJ, Baran N, Stuart ME, Ward RS (2012) Emerging organic contaminants in groundwater: a review of sources, fate and occurrence. Environ Pollut 163:287–303

    Article  CAS  Google Scholar 

  • Lin AY-C, Tsai Y-T (2009) Occurrence of pharmaceuticals in Taiwan’s surface waters: impact of waste streams from hospitals and pharmaceutical production facilities. Sci Total Environ 407:3793–3802

    Article  CAS  Google Scholar 

  • Lin AY-C, Yu T-H, Lin C-F (2008) Pharmaceutical contamination in residential, industrial, and agricultural waste streams: risk to aqueous environments in Taiwan. Chemosphere 74:131–141

    Article  CAS  Google Scholar 

  • Llorente MT, Martos A, Castano A (2002) Detection of cytogenetic alterations and blood cell changes in natural populations of carp. Ecotoxicology 11:27–34

    Article  CAS  Google Scholar 

  • Nadal-Ginard B (1978) Regulation of lactate dehydrogenase levels in the mouse. J Biol Chem 253(1):170–177.

    CAS  Google Scholar 

  • Nepomuceno JC, Ferrari I, Spanó MA, Centeno AJ (1997) Detection of micronuclei in pheripheral erytrocytes of Cyprinus carpio exposed to metallic mercury. Environ Mol Mutagen 30(3):293–297

    Article  CAS  Google Scholar 

  • Obiakor MO, Okonkwo JC, Nnabude PC, Ezeonyejiaku CD (2012) Eco-genotoxicology: micronucleus assay in fish erythrocytes as in situ aquatic pollution biomarker: a review. J Anim Sci Adv 2(1):123–133

    Google Scholar 

  • Orias F, Perrodin Y (2013) Characterization of the ecotoxicity of hospital effluents: a review. Sci Total Environ 1(454–455):250–276

    Article  Google Scholar 

  • Ort C, Lawrence MG, Reungoat J, Eaglesham G, Carter S, Keller J (2010) Determining the fraction of pharmaceutical residues in wastewater originating from a hospital. Water Res 44:605–615

    Article  CAS  Google Scholar 

  • Petrovich M, Hernando MD, Diaz-Cruz S, Barceló D (2005) Liquid chromatography–tandem mass spectrometry for the analysis of pharmaceutical residues in environmental samples: a review. J Chromatogr A 1067(1–2):1–14

    Article  Google Scholar 

  • Richardson SD, Ternes TA (2011) Water analysis: emerging contaminants and current issues. Anal Chem 83:4614–4648

    Article  CAS  Google Scholar 

  • Sim W-J, Lee J-W, Lee E-S, Shin S-K, Hwang S-R, Oh J-E (2011) Occurrence and distribution of pharmaceuticals in wastewater from households, livestock farms, hospitals and pharmaceutical manufactures. Chemosphere 82:179–186

    Article  CAS  Google Scholar 

  • TR 080: Aquatic Toxicity of Mixtures (2001) European centre for ecotoxicology and toxicology of chemicals. Brussels, Belgium

  • Verlicchi P, Al-Aukidy M, Galletti A, Petrovic M, Barceló D (2012) Hospital effluents: investigation of the concentrations and distribution of pharmaceuticals and environmental risk assessment. Sci Total Environ 430:109–118

    Article  CAS  Google Scholar 

  • Way C (2012) Standard methods for the examination of water and wastewater. Water Environ Federation, Secaucus

    Google Scholar 

  • Yu R, Mandlekar S, Harvey KJ, Ucker DS, Tony-Kong A-N (1998) Chemopreventive isothiocyanates induce apoptosis and caspase-3 like protease activity. Cancer Res 58:402–408

    CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge the National Science and Technology Council (CONACYT) for research funding (Project Reference Number 151665).

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Authors and Affiliations

  1. Facultad de Química, Universidad Autónoma del Estado de México, Paseo Tollocan esq Paseo Colón S/N, C.P. 50120, Toluca, Estado de México, Mexico

    Corina G. Olvera-Néstor, Enrique Morales-Avila, Leobardo M. Gómez-Olivan & Nadia Neri-Cruz

  2. Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico, Mexico

    Marcela Galár-Martínez & Sandra García-Medina

Authors
  1. Corina G. Olvera-Néstor
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  2. Enrique Morales-Avila
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  3. Leobardo M. Gómez-Olivan
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  4. Marcela Galár-Martínez
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  5. Sandra García-Medina
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  6. Nadia Neri-Cruz
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Correspondence to Enrique Morales-Avila.

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Olvera-Néstor, C.G., Morales-Avila, E., Gómez-Olivan, L.M. et al. Biomarkers of Cytotoxic, Genotoxic and Apoptotic Effects in Cyprinus carpio Exposed to Complex Mixture of Contaminants from Hospital Effluents. Bull Environ Contam Toxicol 96, 326–332 (2016). https://doi.org/10.1007/s00128-015-1721-3

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  • DOI: https://doi.org/10.1007/s00128-015-1721-3

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