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Genotoxicity of Water Contaminants from the Basin of Lake Sevan, Armenia Evaluated by the Comet Assay in Gibel Carp (Carassius auratus gibelio) and Tradescantia Bioassays

Abstract

Combination of bioassays and chemical analysis was applied to determine the genotoxic/mutagenic contamination in four different sites of the basin of Lake Sevan in Armenia. Water genotoxicity was evaluated using the single cell gel electrophoresis technique (comet assay) in erythrocytes of gibel carp (Carassius auratus gibelio), Tradescantia micronucleus (Trad-MCN) and Tradescantia stamen hair mutation (Trad-SHM) assays. Significant inter-site differences in the levels of water genotoxicity according to fish and Trad-MCN bioassays have been revealed. Two groups of locations with lower (south-southwest of the village Shorzha and Peninsula of Lake Sevan) and higher (estuaries of Gavaraget and Dzknaget rivers) levels of water genotoxicity were distinguished. Correlation analysis support the hypothesis that the observed genetic alterations in fish and plant may be a manifestation of the effects of water contamination by nitrate ions, Si, Al, Fe, Mn and Cu. Increase of DNA damage in fish also correlated with content of total phosphorus.

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References

  1. Agyemang TK, Heblinski J, Schmieder K, Sajadyan H, Vardanyan L (2011) Accuracy assessment of supervised classification of submersed macrophytes: the case of the Gavaraget region of Lake Sevan, Armenia. Hydrobiologia 661:85–96. doi:10.1007/s10750-010-0465-7

    Article  Google Scholar 

  2. American Public Health Association—APHA (1998). In: Clesceri LS, Greenberg AE, Eaton AD (eds) Standard methods for the examination of water and wastewater, 20th edn. Washington, DC, pp 1–1 to 7–49

  3. Babayan A, Hakobyan S, Jenderedjian K, Muradyan S, Voskanov M (2005) Lake Sevan: experience and lessons learned brief. Lake Basin Management Initiative (LBMI). ILEC Foundation. Otsu Shigyo, Otsu, pp 347–362

  4. Danielyan K, Gabrielyan B, Minasyan S, Chilingaryan L, Melkonyan G, Karakhanyan A, Tozalakyan P, Vanyan A, Pirumyan G, Ghukasyan E, Sarksyan L (2011) Integral evaluation of environmental status of Lake Sevan, Yerevan. “SHD”/UNEPCom, pp 1–42 (in Russian)

  5. Duan CQ, Hu B, Wang ZH, Wen CH, Yan SQ, Jiang XH, Wang DK, Li Q, Liang XF (1999) Tradescantia bioassays for the determination of genotoxicity of water in the Panlong River, Kunming, People’s Republic of China. Mutat Res 426:127–131

    Article  CAS  Google Scholar 

  6. Gabbianelli R, Lupidi G, Villarini M, Falcioni G (2003) DNA damage induced by copper on erythrocytes of gilthead sea bream Sparus aurata and mollusk Scapharcaina equivalvis. Arch Environ Contam Toxicol 45:350–356

    CAS  Google Scholar 

  7. Jha AN (2008) Ecotoxicological applications and significance of the comet assay. Mutagenesis 23:207–221. doi:10.1093/mutage/gen014

    Article  CAS  Google Scholar 

  8. Kopjar N, Mustafić P, Zanella D, Buj I, Ćaleta M, Marčić Z, Milić M, Dolenec Z, Mrakovčić M (2008) Assessment of DNA integrity in erythrocytes of Cobitis elongata affected by water pollution: the alkaline comet assay study. Folia Zool 57:120–130

    Google Scholar 

  9. Ma TH, Cabrera GL, Cebulska-Wasilewska A, Cabrera GL, Loarca F, Vandenberg AL, Salamone MF (1994a) Tradescantia stamen hair mutation bioassay. Mutat Res 310:211–220

    Article  CAS  Google Scholar 

  10. Ma TH, Cabrera GL, Chen R, Gill BS, Sandhu SS, Vandenberg AL, Salamone MF (1994b) Tradescantia micronucleus bioassay. Mutat Res 310:221–230

    Article  CAS  Google Scholar 

  11. Misík M, Ma TH, Nersesyan A, Monarca S, Kim JK, Knasmueller S (2011) Micronucleus assays with Tradescantia pollen tetrads: an update. Mutagenesis 26:215–221

    Article  CAS  Google Scholar 

  12. Mišík M, Burke IT, Reismüller M, Pichler C, Rainer B, Mišíková K, Mayes WM, Knasmueller S (2014) Red mud a byproduct of aluminum production contains soluble vanadium that causes genotoxic and cytotoxic effects in higher plants. Sci Total Environ 493:883–890

    Article  CAS  Google Scholar 

  13. Ohe T, Watanabe T, Wakabayashi K (2004) Mutagens in surface waters: a review. Mutat Res 567:109–149

    Article  CAS  Google Scholar 

  14. Radić S, Gregorović G, Stipaničev D, Cvjetko P, Srut M, Vujčić V, Oreščanin V, Vinko Klobučar GI (2013) Assessment of surface water in the vicinity of fertilizer factory using fish and plants. Ecotoxicol Environ Saf 96:32–40. doi:10.1016/j.ecoenv.2013.06.023

    Article  CAS  Google Scholar 

  15. Sasaki YF, Izumiyama F, Nishidate E, Ishibashi S, Tsuda S, Matsusaka N, Asano N, Saotome K, Sofuni T, Hayashi M (1997) Detection of genotoxicity of polluted sea water using shellfish and the alkaline single-cell gel electrophoresis (SCE) assay: a preliminary study. Mutat Res 393:133–139

    Article  CAS  Google Scholar 

  16. Scalon MC, Rechenmacher C, Siebel AM, Kayser ML, Rodrigues MT, Maluf SW, Rodrigues MA, Silva LB (2013) Genotoxic potential and physicochemical parameters of Sinos River, southern Brazil. Sci World J. doi:10.1155/2013/209737

    Google Scholar 

  17. Ternjej I, Mihaljević Z, Stanković I, Kerovec M, Sipos L, Zeljezić D, Kopjar N (2010) Estimation of DNA integrity in blood cells of eastern mosquitofish (Gambusia holbrooki) inhabiting an aluminium-polluted water environment: an alkaline comet assay study. Arch Environ Contam Toxicol 59:182–193. doi:10.1007/s00244-010-9469-6

    Article  CAS  Google Scholar 

  18. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206–221

    Article  CAS  Google Scholar 

  19. Yang G (1999) Tradescantia-micronucleus assay on the water quality of lake Hongzhe in Jiangsu Province, China. Mutat Res 426:155–157

    Article  CAS  Google Scholar 

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Acknowledgments

This research program (10-3/I-9) was funded by State committee of science (Ministry of Education and Science of Armenia).

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Correspondence to Rouben Aroutiounian.

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Simonyan, A., Gabrielyan, B., Minasyan, S. et al. Genotoxicity of Water Contaminants from the Basin of Lake Sevan, Armenia Evaluated by the Comet Assay in Gibel Carp (Carassius auratus gibelio) and Tradescantia Bioassays. Bull Environ Contam Toxicol 96, 309–313 (2016). https://doi.org/10.1007/s00128-015-1720-4

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Keywords

  • Comet assay
  • Fish
  • Tradescantia bioassays
  • Biomonitoring