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Evaluation of Inhibition Efficiency for the Detection of Captan, 2,3,7,8-Tetrachlorodibenzodioxin, Pentachlorophenol and Carbosulfan in Water: An Electrochemical Approach

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Abstract

A novel bio-analytical method has been devised based on the change in catalytic activity of acetylcholinesterase (AChE) enzyme induced by captan, carbosulfan, 2,3,7,8-tetrachlorodibenzodioxin (TCDD) and pentachlorophenol (PCP) for the investigation of inhibition efficiency and sensitivity using Pt/ZnO/AChE/Chitosan bioelectrode. The inhibition curves of captan, carbosulfan, TCDD and PCP were similar to Michaelis–Menten curve. TCDD held the minimum inhibitor Michaelis–Menten constant (\(K_{M}^{I}\)) value (10.2 nM) in comparison with PCP (10.9 nM), carbosulfan (14.5 nM) and captan (7.9 × 103 nM). The maximum inhibition of AChE enzyme by captan was about 100 %, which was much higher than that of TCDD (72.7 %), PCP (68.1 %) and carbosulfan (47.7 %). The calculated theoretical sensitivity was in the order of TCDD > PCP > carbosulfan > captan. Comparing with TCDD (35.3 %), PCP (47.8 %) and carbosulfan (20.9 %), only the inhibition efficiency of captan (55.0 %) was the maximum. The developed bioelectrode exhibited high recovery and low relative standard deviation in local tap water samples.

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Acknowledgments

The authors are grateful to the Department of Science and Technology, New Delhi, for their financial support [DST/TSG/PT/2008/28, SR/FST/ETI-284/2011(C) and (Nano Mission Council (No. SR/NM/PG-16/2007)]. The research was supported by Professor T. R. Rajagopalan research fund, SASTRA University. We also acknowledge SASTRA University, Thanjavur for extending infrastructural support to carry out the study.

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Authors and Affiliations

  1. Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India

    Noel Nesakumar, Bhat Lakshmishri Ramachandra & John Bosco Balaguru Rayappan

  2. Centre for Nanotechnology and Advanced Biomaterials (CeNTAB), School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India

    Noel Nesakumar, Bhat Lakshmishri Ramachandra, Swaminathan Sethuraman, Uma Maheswari Krishnan & John Bosco Balaguru Rayappan

  3. School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India

    Swaminathan Sethuraman & Uma Maheswari Krishnan

  4. School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India

    Noel Nesakumar, Bhat Lakshmishri Ramachandra & John Bosco Balaguru Rayappan

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  1. Noel Nesakumar
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  2. Bhat Lakshmishri Ramachandra
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  3. Swaminathan Sethuraman
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  4. Uma Maheswari Krishnan
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  5. John Bosco Balaguru Rayappan
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Correspondence to John Bosco Balaguru Rayappan.

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Nesakumar, N., Ramachandra, B.L., Sethuraman, S. et al. Evaluation of Inhibition Efficiency for the Detection of Captan, 2,3,7,8-Tetrachlorodibenzodioxin, Pentachlorophenol and Carbosulfan in Water: An Electrochemical Approach. Bull Environ Contam Toxicol 96, 217–223 (2016). https://doi.org/10.1007/s00128-015-1705-3

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  • DOI: https://doi.org/10.1007/s00128-015-1705-3

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