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Survival, Reproduction, Avoidance Behavior and Oxidative Stress Biomarkers in the Earthworm Octolasion cyaneum Exposed to Glyphosate

Abstract

The massive use of glyphosate (GLY) in several countries has increased the interest in investigating its potential adverse effects in non-target organisms. The aim of the present study was to assess the potential effects in survival and reproduction; avoidance behavior and oxidative stress under short-term (48 h) and subchronic exposures (28 days) to GLY in the earthworm Octolasion cyaneum. After 48 h no significant changes in the behavior was observed. In addition, a lower catalase activity at 498 μg GLY kg−1 dry soil section relative to earthworms from the control section was obtained. After 28 days of exposure inhibition of glutathione S-transferase activity was observed at 535 μg GLY kg−1 dry soil while no changes in the other endpoints were detected. These results indicate that environmentally relevant concentrations of GLY (up to 996 µg GLY kg−1 dry soil) did not exert a toxic effect to O. cyaneum.

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Acknowledgments

Thank you to N. Clemente for the statistical analysis. This work was supported by CONICET (PIP 112-201-101-01084) and UNMdP (EXA 608/12 and AGR 390/12).

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Correspondence to Mirta L. Menone.

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Salvio, C., Menone, M.L., Rafael, S. et al. Survival, Reproduction, Avoidance Behavior and Oxidative Stress Biomarkers in the Earthworm Octolasion cyaneum Exposed to Glyphosate. Bull Environ Contam Toxicol 96, 314–319 (2016). https://doi.org/10.1007/s00128-015-1700-8

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Keywords

  • Oligochaete
  • Avoidance behavior
  • Biochemical biomarkers
  • Herbicide