Skip to main content

Influence of Short Term Exposure of TBT on the Male Reproductive Activity in Freshwater Prawn Macrobrachium rosenbergii (De Man)

Bulletin of Environmental Contamination and Toxicology volume 93, pages 446–451 (2014)Cite this article

Abstract

In the present study, the effect of tributyltin (TBT) on the histopathological and hormonal changes during spermatogenesis in freshwater prawn Macrobrachium rosenbergii was documented. Three experimental concentrations such as 10, 100 and 1,000 ng/L were selected and exposed to prawns for 45 days. After TBT exposure, the reproductive activities like sperm count and sperm length were decreased when compared with control. Further, abnormal structure of the seminiferous tubule, decrease in spermatozoa concentration, diminution of the seminiferous tubule membrane and the abundance of spermatocytes in the testis were noticed in treated prawns. Interestingly, radioimmunoassay clearly revealed the reduction of testosterone level in TBT exposed groups. Thus, TBT has considerably reduced the level of testosterone and caused the impairment of spermatogenesis in the freshwater male prawn M. rosenbergii.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3

References

  • Baldwin WS, LeBlane GA (1994) Identification of multiple steroid hydroxylases in Daphnia magna and their modulation by xenobiotics. Environ Toxicol Chem 13:1013–1021

    CAS  Article  Google Scholar 

  • Chiliamovitch VP, Kuhn C (1977) Behavioral, hematological studies on acute toxicity of bis (tri-n-butyltin) oxide on Salmo gairdneri Richardson and Tilapia rendalli Boulenger. J Fish Biol 10:575–585

    Article  Google Scholar 

  • Duncan J (1980) The toxicology of molluscidies. Organotin Pharm Ther 10:407–429

    CAS  Article  Google Scholar 

  • Dutta GJ (1996) Uridine diphosphate glucose and the synthesis of glucosides by mollusks. Arch Biochem Biophysiol 116(1):399–405

    Google Scholar 

  • FAO FAO/UNOP (1986) Meeting on the effect of pollution on marine ecosystem. FAO. Fish Report 352:20

  • Fent K (1996) Ecotoxicology of organotin compounds. Cri Rev Toxicol 26:1–117

    CAS  Google Scholar 

  • Fent K, Hunn J (1995) Organotins in freshwater harbours and river-temporal distribution, annual trends and fate. J Environ Toxicol Chem 14:1123–1132

    CAS  Article  Google Scholar 

  • Haubruge E, Petit F, Gage MJG (2000) Reduced sperm counts in guppies (Poecilia reticulate) following exposure to low levels of Tributyltin and Bisphenol A. Biol Sci 267:2333–2337

    CAS  Article  Google Scholar 

  • Janer G, Sternberg RM, LeBlanc GA, Porte C (2005) Testosterone conjugating activities in invertebrates: are they targets for endocrine disruptors? Aquat Toxicol 71:273–282

    CAS  Article  Google Scholar 

  • Jegou B (1992) The sertoli cells in vivo and in vitro. Cell Biol Toxicol 8:49–54

    CAS  Article  Google Scholar 

  • Kharat PS, Laxmi I, Ghoble B, Shejule KB, Ghoble IBC (2009) Effect of TBTCL on glycogen profile in freshwater prawn, Macrobrachium kistnensis. World App Sci J 7(12):1534–1539

  • Kumar M, Trivedi SP, Misra A, Sharma S (2007) Histopathological changes in testis of the freshwater fish, Heteropneustes fossilis (Bloch) exposed to linear alkyl benzene sulphonate (LAS). J Environ Biol 28:679–684

    CAS  Google Scholar 

  • Lavado R, Barbaglio A, Candia Carnevali MD, Porte C (2006) Steroid levels in crinoid echinoderms are altered by exposure to model endocrine disruptors. Steroids 71:489–497

    CAS  Article  Google Scholar 

  • Mansueto V, Vittoria CM, Faqi AS (2011) Post-embryonic development effect of bisphenol A and tributyltin effects in Ciona intestinalis. Caryologia 64(4):478–484

    Google Scholar 

  • Michel P, Averty B (1991) Tributyltin analysis in seawater by GC-FPD after direct aqueous phase ethylation using sodium tetraethyl borate. Appl Organomet Chem 5:393–397

    CAS  Article  Google Scholar 

  • Morcillo Y, Porte C (1997) Interaction of tributyl and triphenyltin with the microsomal monooxygenase system of mollusks and fish from the western Mediterranean. Aquat Toxicol 38:35–46

    CAS  Article  Google Scholar 

  • Nakayama K, Oshima Y, Yamaguchi T, Tsuruda Y, Kang IJ, Kobayashi M, Imada N, Honjo T (2004) Fertilization success and sexual behavior in male medaka, Oryzias latipes exposed to tributyltin. Chemosphere 55:1331–1337

    CAS  Article  Google Scholar 

  • Oreczyk GP, Caldwell BV, Behrman HR (1974) Endocrinology. In: Jaffe BM, Behrman HR (eds) Methods of hormone radioimmunoassay. New York, Academic press, pp 256–258

  • Parks LG, LeBlanc GA (1996) Reductions in steroid hormone biotransformation/elimination as a biomarker of pentachlorophenol chronic toxicity. Aquat Toxicol 34:291–303

    CAS  Article  Google Scholar 

  • Revathi P (2010) Studies on the endocrine disruptor and its impact on the reproductive physiology of the freshwater prawn Macrobrachium rosenbergii (De Man). Ph. D., Thesis, University of Madras. Chennai, Tamil nadu, India

  • Revathi P, Munuswamy N (2010) Effect of TBT on the early embryonic development in the freshwater prawn Macrobrachium rosenbergii (De Man). Chemosphere 79:922–927

    CAS  Article  Google Scholar 

  • Revathi P, Iyapparaj P, Arockia Vasanthi L, Munuswamy N, Krishnan M (2013a) Impact of TBT on the vitellogenesis and sex hormones in freshwater prawn, Macrobrachium rosenbergii (De Man, 1879). Aquat Biosyst 9:10

    Article  Google Scholar 

  • Revathi P, Iyapparaj P, Arockia Vasanthi L, Munuswamy N, Krishnan M (2013b). Ultra structural changes during spermatogenesis, biochemical and hormonal evidences of testicular toxicity caused by TBT in prawn Macrobrachium rosenbergii (De Man). Environ Toxicol. doi:10.1002/tox.21848

  • Revathi P, Iyapparaj P, Arockia Vasanthi L, Munuswamy N, Krishnan M (2013c) Bioaccumulation of tributyltin and its impact on spermatogenesis in mud crab Scylla serrata (Forskal). Turk J Biol 37:296–304

    Google Scholar 

  • Ruby SM, Idler DR, So YP (1986) The effect of sublethal cyanide exposure on plasma vitellogenesis levels in rainbow trout, Salmo gaidneri during early vitellogenesis. Arch Environ Contam Toxicol 15:603–607

    CAS  Article  Google Scholar 

  • Ruby SM, Idler DR, So YP (1987) Changes in plasma, liver and ovary vitellogenin in land-locked atlantic salmon following exposure to sublethal cyanide. Arch Environ Contam Toxicol 16:507–510

    CAS  Article  Google Scholar 

  • Sokal RZ, Madding CE, Swerdloff RS (1985) Lead toxicity and the hypothalamic pitutary testicular axis. Biol Reprod 33:722–728

    Article  Google Scholar 

  • Srivastava T, Yadav K, Sunil Trivedi P, Devi K (2008) Cyprin induced gonadal impairment in a freshwater food fish, Channa punctatus (Bloch). J Environ Biol 29(2):187–191

    CAS  Google Scholar 

  • Tomar NS (1970) Artificial Insemination and reproduction of cattle and buffalo, 2nd edn. Saroj Prakashan, Katra, Allahabad-2

  • Verslycke T, Vercauteren J, DeVos C, Moens L, Sandra P, Janssen CR (2003a) Cellular energy allocation in the estuarine mysid shrimp Neomysis integer (Crustacea: Mysidacea) following tributyltin exposure. J Exp Mar Biol Ecol 288:167–179

    CAS  Article  Google Scholar 

  • Verslycke T, Poelmans S, De Wasch K, Vercauteren J, Devos CMoens L, Sandra P, De Brabander HF, Janssen CR (2003b) Testosterone metabolism in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following tributyltin exposure. Environ Toxicol Chem 22:2030–2036

    CAS  Article  Google Scholar 

  • Weis JS, Kim K (1988) Tributyltin is a teratogen in producing deformities in limbs of the fiddler crab, Uca pugilator. Arch Environ Contam Toxicol 17:583–587

    CAS  Article  Google Scholar 

  • Weis JS, Gottlieb J, Kwiatkowski J (1987) Tributyltin retards regeneration and produces deformities of limbs in the fiddler crab, Uca pugilator. Arch Environ Contam Toxicol 16:321–326

    CAS  Article  Google Scholar 

  • Zhang J, Zhenghong Z, Yixin C, Yang Z, Shuai H, Chonggang W (2007) Effect of tributyltin on the development of ovary in female cuvier (Sebasticus marmoratus). Aquat Toxicol 83:174–179

    CAS  Article  Google Scholar 

  • Zheng RH, Wang CG, Zhao Y, Zuo ZH, Chen YX (2005) Effect of tributyltin, benzo(a)pyrene and their mixture exposure on the sex hormone levels in gonads of cuvier (Sebastiscus marmoratus). Environ Toxicol Pharma 20:361–367

    CAS  Article  Google Scholar 

  • Zuo Z, Wang C, Wu M, Wang Y, Chen Y (2012) Exposure to tributyltin and triphenyltin induces DNA damage and alters nucleotide excision repair gene transcription in Sebastiscus marmoratus liver. Aquat Toxicol 122–123:106–112

    Article  Google Scholar 

  • Zutshi B (2005) Ultrastructure studies on the effect of fenthion on pituitary (GTH cells) and testis of Glossogobius giuris (Ham) during breeding phase. J Environ Biol 26(1):31–36

  • Zutshi B, Murthy PS (2001) Ultrastructural changes in testis of gold fish Glossogobius giuris (Ham.) induced by fenthion. Ind J Exp Bio 39:170–173

    CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to acknowledge sincere thanks to Dr. D. S. Kothari Post Doctoral Fellowship scheme of University Grants Commission (UGC), India for funding.

Author information

Authors and Affiliations

  1. Department of Environmental Biotechnology, Bharathidasan University, Trichy, 620 024, Tamil Nadu, India

    Peranandam Revathi, Lourduraj Arockia Vasanthi, Vimalanathan Arun Prasanna, Jayaraj Pandiyarajan & Muthukalingan Krishnan

  2. Department of Zoology, University of Madras, Guindy Campus, Chennai, 600 025, Tamil Nadu, India

    Natesan Munuswamy

  3. CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608 502, Tamil Nadu, India

    Palanisamy Iyapparaj

Authors
  1. Peranandam Revathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Palanisamy Iyapparaj
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lourduraj Arockia Vasanthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Natesan Munuswamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Vimalanathan Arun Prasanna
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jayaraj Pandiyarajan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Muthukalingan Krishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Peranandam Revathi.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Revathi, P., Iyapparaj, P., Arockia Vasanthi, L. et al. Influence of Short Term Exposure of TBT on the Male Reproductive Activity in Freshwater Prawn Macrobrachium rosenbergii (De Man). Bull Environ Contam Toxicol 93, 446–451 (2014). https://doi.org/10.1007/s00128-014-1332-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-014-1332-4

Keywords

  • Macrobrachium rosenbergii
  • Tributyltin
  • Spermatogenesis
  • Male reproductive activity
  • Testosterone