Skip to main content
SpringerLink
Log in

Development and Validation of an HPLC Method for Determination of Amikacin in Water Samples by Solid Phase Extraction and Pre-column Derivatization

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

This work presents a rapid and sensitive high performance liquid chromatography method for the determination of amikacin in water samples with solid phase extraction and pre-column derivatization. Amikacin residue was extracted from water samples with solid phase extraction cartridge. Then the extraction solution was derivatized with 4-chloro-3,5-dinitrobenzotrifluoride in the presence of triethylamine at 70°C in 20 min. The amikacin derivative was separated on a C18 column and detected by application of UV detection at 238 nm. The limit of detection is 0.2 μg/L with a signal-to-noise ratio of 3 and linearity is established over the concentration range from 0 to 500.0 μg/L. Recoveries of the amikacin in three types of water samples are from 87.5 % to 99.6 % and RSDs are 2.1 %–4.5 %. This method can be used for the quantification of amikacin residues in water samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Al-Majed AA (2008) A new LC method for determination of some aminoglycoside antibiotics in dosage forms and human plasma using 7-fluoro-4-nitrobenz-2-oxa-1,3-diazole as a fluorogenic pre-column label. Chromatographia 68:927–934

    Article  CAS  Google Scholar 

  • Andrzej P, Jan Z, Jolanta N (2001) Sample preparation for residue determination of gentamicin and neomycin by liquid chromatography. J Chromatogr A 914:59–66

    Article  Google Scholar 

  • Baranowska I, Markowski P, Baranowski J (2006) Simultaneous determination of 11 drugs belonging to four different groups in human urine samples by reversed-phase high-performance liquid chromatography method. Anal Chim Acta 570:46–58

    Article  CAS  Google Scholar 

  • Bates RD, Nahata MC (1994) Once-daily administration of aminoglycosides. Ann Pharmacother 28:757–766

    CAS  Google Scholar 

  • Boczek LA, Rice EW, Johnston B, Johnson JR (2007) Occurrence of antibiotic-resistant uropathogenic Escherichia coli clonal group A in wastewater effluents. Appl Environ Microbiol 73:4180–4184

    Article  CAS  Google Scholar 

  • Brajanoski G, Hoogmartens J, Allegaert K, Adams E (2008) Determination of amikacin in cerebrospinal fluid by high-performance liquid chromatography with pulsed electrochemical detection. J Chromatogr B 867:149–152

    Article  CAS  Google Scholar 

  • De Souza SML, De Vasconcelos EC, Dziedzic M, De Oliveira CMR (2009) Environmental risk assessment of antibiotics: an intensive care unit analysis. Chemosphere 77:962–967

    Article  Google Scholar 

  • Diwan V, Tamhankar AJ, Khandal RK, Sen S, Aggarw-Marothi Y, Vlyer R, Sundblad-Tonderski K, Stalsby-Lundborg C (2012) Antibiotics and antibiotic-resistant bacteria in waters associated with a hospital in Ujjain, India. BMC Public Health 10:414–421

    Article  Google Scholar 

  • Feng CH, Lin SJ, Wu HL, Chen SH (2001a) Trace analysis of amikacin in human plasma by high-performance liquid chromatography. Chromatographia 53:S213–S217

    Article  CAS  Google Scholar 

  • Feng CH, Lin SJ, Wu HL, Chen SH (2001b) Trace analysis of amikacin in commercial preparation by derivatization and HPLC. J Liq Chromatogr Relat Technol 24:381–392

    Article  CAS  Google Scholar 

  • He S, Chen Q, Sun Y, Zhu Y, Luo L, Li J, Cao Y (2011) Determination of tobramycin in soil by HPLC with ultrasonic-assisted extraction and solid-phase extraction. J Chromatogr B 879:901–907

    Article  CAS  Google Scholar 

  • Jakobsen L, Sandvang D, Hansen LH, Bagger-Skjøt L, Westh H, Jørgensen C, Hansen DS, Pedersen BM, Monnet DL, Frimodt-Møller N, Sørensen SJ, Hammerum AM (2008) Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment. Environ Int 34:108–115

    Article  CAS  Google Scholar 

  • Kümmerer KV (2009) Antibiotics in the aquatic environment—a review. Chemosphere 75:417–434

    Article  Google Scholar 

  • Löffler D, Ternes TA (2003) Analytical method for the determination of the aminoglycoside gentamicin in hospital wastewater via liquid chromatography-electrospray-tandem mass spectrometry. J Chromatogr A 1000:583–588

    Article  Google Scholar 

  • Lung KR, Kassal KR, Green JS, Hovsepian PK (1998) Catalytic precolumn derivatization of amikacin. J Pharm Biomed Anal 16:905–910

    Article  CAS  Google Scholar 

  • Matyar F (2012) Antibiotic and heavy metal resistance in bacteria isolated from the eastern Mediterranean Sea coast. Bull Environ Contam Toxicol 89:551–556

    Article  CAS  Google Scholar 

  • Mayhew JW, Gorbach SL (1978) Gas-liquid chromatographic method for the assay of aminoglycoside antibiotics in serum. J Chromatogr A 151:133–146

    Article  CAS  Google Scholar 

  • Nicoli S, Santi P (2006) Assay of amikacin in the skin by high-performance liquid chromatography. J Pharm Biomed Anal 41:994–997

    Article  CAS  Google Scholar 

  • Ovalles JF, Brunetto MR, Gallignani M (2005) A new method for the analysis of amikacin using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization and high performance liquid chromatography with UV-detection. J Pharm Biomed Anal 39:294–298

    Article  CAS  Google Scholar 

  • Posyniak A, Zmudzki J, Semeniuk S (2001) Effects of the matrix and sample preparation on the determination of fluoroquinolone residues in animal tissues. J Chromatogr A 914:89–94

    Article  CAS  Google Scholar 

  • Qian K, Tang T, Shi TY, Wang F, Li JQ, Cao YS (2009) Residue determination of glyphosate in environmental water samples with high-performance liquid chromatography and UV detection after derivatization with 4-chloro-3,5-dinitrobenzotrifluoride. Anal Chim Acta 635:222–226

    Article  CAS  Google Scholar 

  • Sánchez-Martínez ML, Aguilar-Caballos MP, Gómez-Hens A (2009) Long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples. Talanta 78:305–309

    Article  Google Scholar 

  • Serrano JM, Silva M (2006) Rapid and sensitive determination of aminoglycoside antibiotics in water samples using a strong cation-exchange chromatography non-derivatisation method with chemiluminescence detection. J Chromatogr A 1117:176–183

    Article  CAS  Google Scholar 

  • Shi TY, Tang T, Qian K, Wang F, Li JQ, Cao YS (2009) High-performance liquid chromatographic method for determination of amino acids by precolumn derivatization with 4-chloro-3,5-dinitrobenzotrifluoride. Anal Chim Acta 654:154–161

    Article  CAS  Google Scholar 

  • Shi Y, Gao L, Li W, Liu J, Cai Y (2012) Investigation of fluoroquinolones, sulfonamides and macrolides in long-term wastewater irrigation soil in Tianjin, China. Bull Environ Contam Toxicol 89:857–861

    Article  CAS  Google Scholar 

  • Sun Y, Liu PF, Wang D, Li JQ, Cao YS (2009) Determination of amitrole in environmental water samples with precolumn derivatization by high-performance liquid. J Agric Food Chem 57:4540–4544

    Article  CAS  Google Scholar 

  • Wichert B, Schreier H, Derendorf H (1991) Sensitive liquid chromatography assay for the determination of amikacin in human plasma. J Pharm Biomed Anal 9:251–254

    Article  CAS  Google Scholar 

  • Yu CZ, He YZ, Fu GN, Xie HY, Gan WE (2009) Determination of kanamycin A, amikacin and tobramycin residues in milk by capillary zone electrophoresis with post-column derivatization and laser-induced fluorescence detection. J Chromatogr B 877:333–338

    Article  CAS  Google Scholar 

  • Zong H, Ma D, Wang J, Hu J (2010) Research on florfenicol residue in coastal area of Dalian (northern China) and analysis of functional diversity of the microbial community in marine sediment. Bull Environ Contam Toxicol 84:245–249

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by National Department Public Benefit Research Foundation (201003065), Research Fund for the Doctoral Program of Higher Education of China (20100008110044) and Chinese Universities Scientific Fund (2013YJ001) and Scholarship Award for Excellent Doctoral Student granted by Ministry of Education.

Author information

Authors and Affiliations

  1. College of Agriculture and Biotechnology, China Agricultural University, Beijing, China

    Deguang Li, Shun He, Yufang Deng, Guanglong Ding, Hanwen Ni & Yongsong Cao

  2. Beijing Industrial Technology Research Institute, Beijing, China

    Deguang Li

Authors
  1. Deguang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shun He
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yufang Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guanglong Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hanwen Ni
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yongsong Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yongsong Cao.

Additional information

Deguang Li and Shun He have contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, D., He, S., Deng, Y. et al. Development and Validation of an HPLC Method for Determination of Amikacin in Water Samples by Solid Phase Extraction and Pre-column Derivatization. Bull Environ Contam Toxicol 93, 47–52 (2014). https://doi.org/10.1007/s00128-014-1257-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-014-1257-y

Keywords

Search

Navigation