Effects of Glyphosate on Growth Rate, Metabolic Rate and Energy Reserves of Early Juvenile Crayfish, Cherax quadricarinatus M.
- 502 Downloads
Early juveniles of the crayfish Cherax quadricarinatus were exposed for 60 days to 10 and 40 mg/L of pure glyphosate (acid form) in freshwater. Mortality was 33 % at the highest concentration, while no differences in molting were noted among treatments. After the first month of exposure, weight gain was significantly (p < 0.05) reduced in the 40 mg/L group. At the end of the assay, lipid levels in muscle, as well as protein level in both hepatopancreas and muscle were significantly (p < 0.05) reduced. These results suggest long-term utilization of both lipid and protein as main energetic reserves, likely in response to the chronic stress associated with herbicide exposure. Besides, the lower pyruvate kinase activity in muscle suggests a possible metabolic depression in this tissue. The hemolymphatic ASAT:ALAT ratio showed higher levels than the control at the highest glyphosate concentration, indicating possible damage to several tissues.
KeywordsCrayfish Somatic growth Glyphosate Metabolism Enzymes
This work was supported by Grants from ANPCYT (PICT 2010-0908), UBACYT 2012-2015 (code 044) and 2011–2014 scientific programs, UBACYT 2011–2014 (code 01/W985), and PIP CONICET (2010–2012 program, code 100884). We also thank Dr. Itzick Vatnick for style revision.
- American Public Health Association, American Water Works Association, Water Pollution Control Federation (2005) Standard methods for the examination of water and wastewaters, 21st edn. American Public Health Association, Washington, p 1200Google Scholar
- Giesy JP, Dobson S, Solomon KR (2000) Ecotoxicological risk assessment for roundup herbicide. Rev Environ Contam Toxicol 167:35–120Google Scholar
- Jones CM (1997) The biology and aquaculture potential of the tropical freshwater crayfish Cherax quadricarinatus. Dept. of Primary Industries, Brisbane p 109Google Scholar
- Mayer FL, Versteeg DJ, McKee MJ, Folmar LC, Graney RL, McCume DC, Rattner BA (1992) Physiological and nonspecific biomarkers. In: Huggett RJ, Kimerle RA, Mehrle PM, Bergman HL (eds) Biomarkers, biochemical, physiological, and histological markers of anthropogenic stress. Lewis Publishers, Boca Raton, pp 5–85Google Scholar
- Reitman S, Frankel SJ (1957) Determination of aspartate amino transferase activity in blood serum and tissues. Am J Clin Pathol 28:56Google Scholar
- Sokal RR, Rohlf FJ (1981) Biometry, 2nd edn. W.H. Freeman and Company, New York, p 832Google Scholar