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Evaluation of the Interactive Effects of Lead, Zinc and Benzo(k)fluoranthene on Crucian Carp, Carassius carassius, Using a Multiple Biomarker Approach

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Abstract

The aim of this study was to evaluate the interactive effects of the metals lead (Pb) and zinc (Zn) and the polycyclic aromatic hydrocarbon benzo(k)fluoranthene (BkF) on crucian carp (Carassius carassius). To this end, various biomarkers in the fish [7-ethoxyresorufin-O-deethylase (EROD); superoxide dismutase (SOD); malondialdehyde (MDA) and metallothioneins (MT)] were measured after 96 h of exposure. When compared to either treatment with Pb and Zn mixtures or BkF-only treatment, the treatment with BkF combined with Pb and Zn resulted in lower responses of EROD and MT synthesis activity, and higher SOD and MDA increases. These results indicated that the co-exposure of metals and polycyclic aromatic hydrocarbons may cause more severe oxidative stress on fish. However, the effect of these interactions on EROD and MT may lead to an underestimation of the actual exposure in the field and thereby should be carefully considered.

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Acknowledgments

This research was financially supported by the National Natural Science Foundation of China (51279061, 51079049).

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Authors and Affiliations

  1. Key Laboratory for Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing, 210098, People’s Republic of China

    Jiannan Ding & Guanghua Lu

  2. NARI Group Corporation, Nanjing, 211106, People’s Republic of China

    Yan Liang

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  1. Jiannan Ding
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  2. Guanghua Lu
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  3. Yan Liang
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Correspondence to Guanghua Lu.

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Ding, J., Lu, G. & Liang, Y. Evaluation of the Interactive Effects of Lead, Zinc and Benzo(k)fluoranthene on Crucian Carp, Carassius carassius, Using a Multiple Biomarker Approach. Bull Environ Contam Toxicol 92, 534–539 (2014). https://doi.org/10.1007/s00128-014-1220-y

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  • DOI: https://doi.org/10.1007/s00128-014-1220-y

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