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Effect of a Glyphosate-Based Herbicide on Gene Expressions of the Cytokines Interleukin-1β and Interleukin-10 and of Heme Oxygenase-1 in European Sea Bass, Dicentrarchus labrax L.

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Abstract

Glyphosate-based herbicides are the most frequently used herbicides in the world. We evaluated the effect of Roundup 360 SL on the expression of interleukin-1β (il-1β), interleukin-10 (il-10) and heme-oxygenase-1 (ho-1) in the gills, intestines and spleen of young European sea bass (Dicentrachus labrax L.), aged 8 mo. A group of fish was exposed to 647 mg/L of Roundup for 96 h. This treatment did not alter gene expression levels of il-1β and il-10 cytokine in the intestines, but significantly lowered both levels in the gills (p = 0.02 and p = 0.04 respectively). Expression levels of ho-1 were increased significantly in the three organs of fish from the treated group (the gills p = 0.04, the intestines p = 0.004 and the spleen p < 0.001). These changes may in turn negatively impact the immune system of European sea bass exposed to Roundup.

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Acknowledgments

The authors thank the marine farm “Tamaris Sud Fish” for their generous donation of fish and Dr. Sandrine Pierre for her reading of the manuscript. We are very grateful to the Conseil Régional PACA (France) and Conseil Général du Var (France) as well as CARTT (Comité d’Aide à la Recherche et aux Transferts Technologiques – France) for their help.

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Authors and Affiliations

  1. EB2M-PROTEE, EA 3819, Université de Toulon, 83957, La Garde, France

    Simone Richard, Nathalie Prévot-D’Alvise, Robert Bunet, Rémy Simide, Sylvain Couvray, Stéphane Coupé & Joël Paul Grillasca

  2. Institut Océanographique Paul Ricard, Ile des Embiez, Le Brusc, 83140, Six Fours Les Plages, France

    Sylvain Couvray

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  1. Simone Richard
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  2. Nathalie Prévot-D’Alvise
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  4. Rémy Simide
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  5. Sylvain Couvray
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  6. Stéphane Coupé
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  7. Joël Paul Grillasca
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Correspondence to Simone Richard.

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Simone Richard and Nathalie Prévot-D’Alvise have contributed equally to this project and should be considered co-first authors.

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Richard, S., Prévot-D’Alvise, N., Bunet, R. et al. Effect of a Glyphosate-Based Herbicide on Gene Expressions of the Cytokines Interleukin-1β and Interleukin-10 and of Heme Oxygenase-1 in European Sea Bass, Dicentrarchus labrax L.. Bull Environ Contam Toxicol 92, 294–299 (2014). https://doi.org/10.1007/s00128-013-1180-7

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  • DOI: https://doi.org/10.1007/s00128-013-1180-7

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