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Persistence and Metabolism of Fipronil in Sugarcane Leaves and Juice


Fipronil gives effective control of early shoot borer and termites in sugarcane. The persistence and metabolism of fipronil in sugarcane leaves and juice were studied following application of fipronil (Regent 0.3 G) at 75 and 300 g a.i. ha−1. Samples of sugarcane leaves were collected at various time intervals. Samples of sugarcane juice were collected at harvest. Residues of fipronil and its metabolites were quantified by gas liquid chromatograph. The limit of quantification of fipronil and its metabolites was 0.01 mg kg−1 for sugarcane leaves and juice. Total residues of fipronil and its metabolites in sugarcane leaves after 7 days of its application at 75 and 300 g a.i. ha−1 were 0.26 and 0.66 mg kg−1, respectively. Residues could not be detected after 60 and 90 following fipronil application at either concentration. In sugarcane leaves, fipronil was found to be the main constituent, followed by its metabolites amide, desulfinyl, sulfone and sulfide. Samples of sugarcane juice did not reveal the presence of fipronil or its metabolites following its application at both the dosages at harvest.

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  1. Aajoud A, Ravanel P, Tissut M (2003) Fipronil metabolism and dissipation in a simplified aquatic ecosystem. J Agric Food Chem 51:1347–1352

    CAS  Article  Google Scholar 

  2. Anonymous (2009) Package of practices for kharif crops. Punjab Agricultural University, Ludhiana, pp 92–110

    Google Scholar 

  3. Anonymous (2011) Package of practices for kharif crops. Punjab Agricultural University, Ludhiana, pp 74–89

    Google Scholar 

  4. Balanca G, del Visscer MN (1997) Effect of very low doses of fipronil on grasshoppers and non target insects following field trials for grasshopper control. Crop Prot 16:553–564

    CAS  Article  Google Scholar 

  5. Battu RS, Kalra RL, Dhillon RS (2000) Estimation of residues of carbofuran and its metabolites in sugarcane and soil by derivatization with 1-fluoro-2,4-dinitrobenzene and gas chromatography with nitrogen-phosphorus detection. J AOAC Int 83:569–578

    CAS  Google Scholar 

  6. Bhardwaj U, Kumar R, Kaur S, Sahoo SK, Mandal K, Battu RS, Singh B (2012) Persistence of fipronil and its risk assessment on cabbage, Brassica oleracea var. capitata L. Ecotoxicol Environ Saf 79:301–308

    CAS  Article  Google Scholar 

  7. Chanton PF, Ravanel P, Tissut M, Meyran JC (2001) Toxicity and bioaccumulation of fipronil in the nontarget arthropodan fauna associated with subalpine mosquito breeding. Ecotoxic Environ Saf 52:8–12

    Article  Google Scholar 

  8. Collins HL, Callcott AMA (1998) Fipronil: an ultra low dose bait toxicant for control of red imported fire ants (Hymenoptera: Formicidae). Florida Ent 8:407–415

    Article  Google Scholar 

  9. Gangwar SK, Tewari RK (2001) Degradation and translocation of phorate residues in soil and in sugarcane. Indian J Ent 63:281–284

    Google Scholar 

  10. Gupta S, Sharma RK, Sinha SR, Gupta RK, Gajbhiye VT (2007) Persistence of some new insecticides in brinjal and their efficacy against brinjal leaf hopper and borer. Pestic Res J 19:205–209

    CAS  Google Scholar 

  11. Hainzl D, Casida JE (1996) Fipronil insecticides: novel photochemical desulfinylation with retention of neurotoxicity. Proc Nat Acad Sci USA 93:12764–12767

    CAS  Article  Google Scholar 

  12. Kharbade SB, Dethe MD (2001) Residues of fipronil in sugarcane. Pestology 25:18–19

    Google Scholar 

  13. Pei Z, Yitong L, Baofeng L, Gan JJ (2004) Dynamics of fipronil residue in vegetable field ecosystem. Chemosphere 57:1691–1696

    CAS  Article  Google Scholar 

  14. Sharma S, Mann RS, Uppal SK (2007) Efficacy of fipronil against early shoot borer and termites of sugarcane. J Insect Sci 20:193–198

    Google Scholar 

  15. Skidmore MW, Ambrus A (2004) Pesticide metabolism in crops and livestock. In: Hamilton D, Crossley S (eds) Pesticide residues in food and drinking water: human exposure and risks. John Wiley & Sons, Ltd, Chichester, pp 63–120

    Google Scholar 

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The authors are thankful to the professor and Head, Department of Entomology, PAU, Ludhiana for providing the necessary research facilities.

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Correspondence to Kousik Mandal.

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Mandal, K., Singh, B. Persistence and Metabolism of Fipronil in Sugarcane Leaves and Juice. Bull Environ Contam Toxicol 92, 220–224 (2014).

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  • Amide
  • Desulfinyl
  • GLC
  • Residues
  • Sulfide
  • Sulfone